Identification of a circulating microRNAs biomarker panel for non-invasive diagnosis of coronary artery disease: case–control study
Abstract Background Circulating microRNAs (miRNAs) are considered a hot spot of research that can be employed for monitoring and/or diagnostic purposes in coronary artery disease (CAD). Since different disease features might be reflected on altered profiles or plasma miRNAs concentrations, a combina...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-06-01
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| Series: | BMC Cardiovascular Disorders |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12872-022-02711-9 |
| _version_ | 1818545953768996864 |
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| author | Hoda Y. Abdallah Ranya Hassan Ahmed Fareed Mai Abdelgawad Sally Abdallah Mostafa Eman Abdel-Moemen Mohammed |
| author_facet | Hoda Y. Abdallah Ranya Hassan Ahmed Fareed Mai Abdelgawad Sally Abdallah Mostafa Eman Abdel-Moemen Mohammed |
| author_sort | Hoda Y. Abdallah |
| collection | DOAJ |
| description | Abstract Background Circulating microRNAs (miRNAs) are considered a hot spot of research that can be employed for monitoring and/or diagnostic purposes in coronary artery disease (CAD). Since different disease features might be reflected on altered profiles or plasma miRNAs concentrations, a combination of miRNAs can provide more reliable non-invasive biomarkers for CAD. Subjects and methods We investigated a panel of 14-miRNAs selected using bioinformatics databases and current literature searching for miRNAs involved in CAD using quantitative real-time PCR technique in 73 CAD patients compared to 73 controls followed by function and pathway enrichment analysis for the 14-miRNAs. Results Our results revealed three out of the 14 circulating miRNAs understudy; miRNAs miR133a, miR155 and miR208a were downregulated. While 11 miRNAs were up-regulated in a descending order from highest fold change to lowest: miR-182, miR-145, miR-21, miR-126, miR-200b, miR-146A, miR-205, miR-135b, miR-196b, miR-140b and, miR-223. The ROC curve analysis indicated that miR-145, miR-182, miR-133a and, miR-205 were excellent biomarkers with the highest AUCs as biomarkers in CAD. All miRNAs under study except miR-208 revealed a statistically significant relation with dyslipidemia. MiR-126 and miR-155 showed significance with BMI grade, while only miR-133a showed significance with the obese patients in general. MiR-135b and miR-140b showed a significant correlation with the Wall Motion Severity Index. Pathway enrichment analysis for the miRNAS understudy revealed pathways relevant to the fatty acid biosynthesis, ECM-receptor interaction, proteoglycans in cancer, and adherens junction. Conclusion The results of this study identified a differentially expressed circulating miRNAs signature that can discriminate CAD patients from normal subjects. These results provide new insights into the significant role of miRNAs expression associated with CAD pathogenesis. |
| first_indexed | 2024-12-12T07:46:42Z |
| format | Article |
| id | doaj.art-47a6e75579e942d7a163508abbca5bb6 |
| institution | Directory Open Access Journal |
| issn | 1471-2261 |
| language | English |
| last_indexed | 2024-12-12T07:46:42Z |
| publishDate | 2022-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cardiovascular Disorders |
| spelling | doaj.art-47a6e75579e942d7a163508abbca5bb62022-12-22T00:32:35ZengBMCBMC Cardiovascular Disorders1471-22612022-06-0122111710.1186/s12872-022-02711-9Identification of a circulating microRNAs biomarker panel for non-invasive diagnosis of coronary artery disease: case–control studyHoda Y. Abdallah0Ranya Hassan1Ahmed Fareed2Mai Abdelgawad3Sally Abdallah Mostafa4Eman Abdel-Moemen Mohammed5Medical Genetics Unit, Department of Histology and Cell Biology, Faculty of Medicine, Suez Canal UniversityDepartment of Clinical Pathology, Faculty of Medicine, Suez Canal UniversityDepartment of Cardiology, Faculty of Medicine, Suez Canal UniversityBiotechnology and Life Sciences Department, Faculty of Postgraduate Studies for Advanced Sciences (PSAS), Beni-Suef UniversityDepartment of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Mansoura UniversityMedical Genetics Unit, Department of Histology and Cell Biology, Faculty of Medicine, Suez Canal UniversityAbstract Background Circulating microRNAs (miRNAs) are considered a hot spot of research that can be employed for monitoring and/or diagnostic purposes in coronary artery disease (CAD). Since different disease features might be reflected on altered profiles or plasma miRNAs concentrations, a combination of miRNAs can provide more reliable non-invasive biomarkers for CAD. Subjects and methods We investigated a panel of 14-miRNAs selected using bioinformatics databases and current literature searching for miRNAs involved in CAD using quantitative real-time PCR technique in 73 CAD patients compared to 73 controls followed by function and pathway enrichment analysis for the 14-miRNAs. Results Our results revealed three out of the 14 circulating miRNAs understudy; miRNAs miR133a, miR155 and miR208a were downregulated. While 11 miRNAs were up-regulated in a descending order from highest fold change to lowest: miR-182, miR-145, miR-21, miR-126, miR-200b, miR-146A, miR-205, miR-135b, miR-196b, miR-140b and, miR-223. The ROC curve analysis indicated that miR-145, miR-182, miR-133a and, miR-205 were excellent biomarkers with the highest AUCs as biomarkers in CAD. All miRNAs under study except miR-208 revealed a statistically significant relation with dyslipidemia. MiR-126 and miR-155 showed significance with BMI grade, while only miR-133a showed significance with the obese patients in general. MiR-135b and miR-140b showed a significant correlation with the Wall Motion Severity Index. Pathway enrichment analysis for the miRNAS understudy revealed pathways relevant to the fatty acid biosynthesis, ECM-receptor interaction, proteoglycans in cancer, and adherens junction. Conclusion The results of this study identified a differentially expressed circulating miRNAs signature that can discriminate CAD patients from normal subjects. These results provide new insights into the significant role of miRNAs expression associated with CAD pathogenesis.https://doi.org/10.1186/s12872-022-02711-9Circulating miRNAsCoronary artery diseaseCAD biomarkermiR-145miR-182miR-133a |
| spellingShingle | Hoda Y. Abdallah Ranya Hassan Ahmed Fareed Mai Abdelgawad Sally Abdallah Mostafa Eman Abdel-Moemen Mohammed Identification of a circulating microRNAs biomarker panel for non-invasive diagnosis of coronary artery disease: case–control study BMC Cardiovascular Disorders Circulating miRNAs Coronary artery disease CAD biomarker miR-145 miR-182 miR-133a |
| title | Identification of a circulating microRNAs biomarker panel for non-invasive diagnosis of coronary artery disease: case–control study |
| title_full | Identification of a circulating microRNAs biomarker panel for non-invasive diagnosis of coronary artery disease: case–control study |
| title_fullStr | Identification of a circulating microRNAs biomarker panel for non-invasive diagnosis of coronary artery disease: case–control study |
| title_full_unstemmed | Identification of a circulating microRNAs biomarker panel for non-invasive diagnosis of coronary artery disease: case–control study |
| title_short | Identification of a circulating microRNAs biomarker panel for non-invasive diagnosis of coronary artery disease: case–control study |
| title_sort | identification of a circulating micrornas biomarker panel for non invasive diagnosis of coronary artery disease case control study |
| topic | Circulating miRNAs Coronary artery disease CAD biomarker miR-145 miR-182 miR-133a |
| url | https://doi.org/10.1186/s12872-022-02711-9 |
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