Transcriptomic Response of the Liver Tissue in <i>Trachinotus ovatus</i> to Acute Heat Stress
<i>Trachinotus ovatus</i> is a major economically important cultured marine fish in the South China Sea. However, extreme weather and increased culture density result in uncontrollable problems, such as increases in water temperature and a decline in dissolved oxygen (DO), hindering the...
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MDPI AG
2023-06-01
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| Online Access: | https://www.mdpi.com/2076-2615/13/13/2053 |
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| author | Qian-Qian Li Jing Zhang Hong-Yang Wang Su-Fang Niu Ren-Xie Wu Bao-Gui Tang Qing-Hua Wang Zhen-Bang Liang Yan-Shan Liang |
| author_facet | Qian-Qian Li Jing Zhang Hong-Yang Wang Su-Fang Niu Ren-Xie Wu Bao-Gui Tang Qing-Hua Wang Zhen-Bang Liang Yan-Shan Liang |
| author_sort | Qian-Qian Li |
| collection | DOAJ |
| description | <i>Trachinotus ovatus</i> is a major economically important cultured marine fish in the South China Sea. However, extreme weather and increased culture density result in uncontrollable problems, such as increases in water temperature and a decline in dissolved oxygen (DO), hindering the high-quality development of aquaculture. In this study, liver transcriptional profiles of <i>T. ovatus</i> were investigated under acute high-temperature stress (31 °C and 34 °C) and normal water temperature (27 °C) using RNA sequencing (RNA-Seq) technology. Differential expression analysis and STEM analysis showed that 1347 differentially expressed genes (DEGs) and four significant profiles (profiles 0, 3, 4, and 7) were screened, respectively. Of these DEGs, some genes involved in heat shock protein (HSPs), hypoxic adaptation, and glycolysis were up-regulated, while some genes involved in the ubiquitin-proteasome system (UPS) and fatty acid metabolism were down-regulated. Our results suggest that protein dynamic balance and function, hypoxia adaptation, and energy metabolism transformation are crucial in response to acute high-temperature stress. Our findings contribute to understanding the molecular response mechanism of <i>T. ovatus</i> under acute heat stress, which may provide some reference for studying the molecular mechanisms of other fish in response to heat stress. |
| first_indexed | 2024-03-11T01:48:55Z |
| format | Article |
| id | doaj.art-47a7a616995f455a9c866c0f0d2b7cd6 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T01:48:55Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-47a7a616995f455a9c866c0f0d2b7cd62023-11-18T16:02:13ZengMDPI AGAnimals2076-26152023-06-011313205310.3390/ani13132053Transcriptomic Response of the Liver Tissue in <i>Trachinotus ovatus</i> to Acute Heat StressQian-Qian Li0Jing Zhang1Hong-Yang Wang2Su-Fang Niu3Ren-Xie Wu4Bao-Gui Tang5Qing-Hua Wang6Zhen-Bang Liang7Yan-Shan Liang8College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Fisheries, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Fisheries, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Fisheries, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Fisheries, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Fisheries, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Fisheries, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Fisheries, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China<i>Trachinotus ovatus</i> is a major economically important cultured marine fish in the South China Sea. However, extreme weather and increased culture density result in uncontrollable problems, such as increases in water temperature and a decline in dissolved oxygen (DO), hindering the high-quality development of aquaculture. In this study, liver transcriptional profiles of <i>T. ovatus</i> were investigated under acute high-temperature stress (31 °C and 34 °C) and normal water temperature (27 °C) using RNA sequencing (RNA-Seq) technology. Differential expression analysis and STEM analysis showed that 1347 differentially expressed genes (DEGs) and four significant profiles (profiles 0, 3, 4, and 7) were screened, respectively. Of these DEGs, some genes involved in heat shock protein (HSPs), hypoxic adaptation, and glycolysis were up-regulated, while some genes involved in the ubiquitin-proteasome system (UPS) and fatty acid metabolism were down-regulated. Our results suggest that protein dynamic balance and function, hypoxia adaptation, and energy metabolism transformation are crucial in response to acute high-temperature stress. Our findings contribute to understanding the molecular response mechanism of <i>T. ovatus</i> under acute heat stress, which may provide some reference for studying the molecular mechanisms of other fish in response to heat stress.https://www.mdpi.com/2076-2615/13/13/2053<i>Trachinotus ovatus</i>liverheat stresstranscriptomic analysisgene expression |
| spellingShingle | Qian-Qian Li Jing Zhang Hong-Yang Wang Su-Fang Niu Ren-Xie Wu Bao-Gui Tang Qing-Hua Wang Zhen-Bang Liang Yan-Shan Liang Transcriptomic Response of the Liver Tissue in <i>Trachinotus ovatus</i> to Acute Heat Stress Animals <i>Trachinotus ovatus</i> liver heat stress transcriptomic analysis gene expression |
| title | Transcriptomic Response of the Liver Tissue in <i>Trachinotus ovatus</i> to Acute Heat Stress |
| title_full | Transcriptomic Response of the Liver Tissue in <i>Trachinotus ovatus</i> to Acute Heat Stress |
| title_fullStr | Transcriptomic Response of the Liver Tissue in <i>Trachinotus ovatus</i> to Acute Heat Stress |
| title_full_unstemmed | Transcriptomic Response of the Liver Tissue in <i>Trachinotus ovatus</i> to Acute Heat Stress |
| title_short | Transcriptomic Response of the Liver Tissue in <i>Trachinotus ovatus</i> to Acute Heat Stress |
| title_sort | transcriptomic response of the liver tissue in i trachinotus ovatus i to acute heat stress |
| topic | <i>Trachinotus ovatus</i> liver heat stress transcriptomic analysis gene expression |
| url | https://www.mdpi.com/2076-2615/13/13/2053 |
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