Early changes of bone metabolites and lymphocyte subsets may participate in osteoporosis onset: a preliminary study of a postmenopausal osteoporosis mouse model
PurposeMetabolic and immune changes in the early stages of osteoporosis are not well understood. This study aimed to explore the changes in bone metabolites and bone marrow lymphocyte subsets and their relationship during the osteoporosis onset.MethodsWe established OVX and Sham mouse models. After...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-02-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2024.1323647/full |
| _version_ | 1797293022149345280 |
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| author | Sizhu Wang Sizhu Wang Yuyou Qiu Cuisong Tang Huan Tang Jinchuan Liu Jieying Chen Lin Zhang Guangyu Tang |
| author_facet | Sizhu Wang Sizhu Wang Yuyou Qiu Cuisong Tang Huan Tang Jinchuan Liu Jieying Chen Lin Zhang Guangyu Tang |
| author_sort | Sizhu Wang |
| collection | DOAJ |
| description | PurposeMetabolic and immune changes in the early stages of osteoporosis are not well understood. This study aimed to explore the changes in bone metabolites and bone marrow lymphocyte subsets and their relationship during the osteoporosis onset.MethodsWe established OVX and Sham mouse models. After 5, 15, and 40 days, five mice in each group were sacrificed. Humeri were analyzed by microCT. The bone marrow cells of the left femur and tibia were collected for flow cytometry analysis. The right femur and tibia were analyzed by LC-MS/MS for metabolomics analysis.ResultsBone microarchitecture was significantly deteriorated 15 days after OVX surgery. Analysis of bone metabolomics showed that obvious metabolite changes had happened since 5 days after surgery. Lipid metabolism was significant at the early stage of the osteoporosis. The proportion of immature B cells was increased, whereas the proportion of mature B cells was decreased in the OVX group. Metabolites were significantly correlated with the proportion of lymphocyte subsets at the early stage of the osteoporosis.ConclusionLipid metabolism was significant at the early stage of the osteoporosis. Bone metabolites may influence bone formation by interfering with bone marrow lymphocyte subsets. |
| first_indexed | 2024-03-07T20:05:54Z |
| format | Article |
| id | doaj.art-47a7fb7fe0fa4d7da9693b65b8f98147 |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-03-07T20:05:54Z |
| publishDate | 2024-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-47a7fb7fe0fa4d7da9693b65b8f981472024-02-28T05:01:40ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922024-02-011510.3389/fendo.2024.13236471323647Early changes of bone metabolites and lymphocyte subsets may participate in osteoporosis onset: a preliminary study of a postmenopausal osteoporosis mouse modelSizhu Wang0Sizhu Wang1Yuyou Qiu2Cuisong Tang3Huan Tang4Jinchuan Liu5Jieying Chen6Lin Zhang7Guangyu Tang8Department of Radiology, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai, ChinaDepartment of Radiology, Guangdong Provincial People’s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong, ChinaDepartment of Radiology, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai, ChinaDepartment of Radiology, Clinical Medical College of Shanghai Tenth People’s Hospital of Nanjing Medical University, Shanghai, ChinaDepartment of Radiology, Huadong Hospital of Fudan University, Shanghai, ChinaDepartment of Obstetrics and Gynaecology, Li Ka Shing (LKS) Faculty of Medicine, The University of Hong Kong, Hong Kong, Hong Kong SAR, ChinaDepartment of Radiology, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai, ChinaDepartment of Radiology, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai, ChinaDepartment of Radiology, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai, ChinaPurposeMetabolic and immune changes in the early stages of osteoporosis are not well understood. This study aimed to explore the changes in bone metabolites and bone marrow lymphocyte subsets and their relationship during the osteoporosis onset.MethodsWe established OVX and Sham mouse models. After 5, 15, and 40 days, five mice in each group were sacrificed. Humeri were analyzed by microCT. The bone marrow cells of the left femur and tibia were collected for flow cytometry analysis. The right femur and tibia were analyzed by LC-MS/MS for metabolomics analysis.ResultsBone microarchitecture was significantly deteriorated 15 days after OVX surgery. Analysis of bone metabolomics showed that obvious metabolite changes had happened since 5 days after surgery. Lipid metabolism was significant at the early stage of the osteoporosis. The proportion of immature B cells was increased, whereas the proportion of mature B cells was decreased in the OVX group. Metabolites were significantly correlated with the proportion of lymphocyte subsets at the early stage of the osteoporosis.ConclusionLipid metabolism was significant at the early stage of the osteoporosis. Bone metabolites may influence bone formation by interfering with bone marrow lymphocyte subsets.https://www.frontiersin.org/articles/10.3389/fendo.2024.1323647/fullosteoporosismetabolismLC-MS/MSlymphocyteB cell |
| spellingShingle | Sizhu Wang Sizhu Wang Yuyou Qiu Cuisong Tang Huan Tang Jinchuan Liu Jieying Chen Lin Zhang Guangyu Tang Early changes of bone metabolites and lymphocyte subsets may participate in osteoporosis onset: a preliminary study of a postmenopausal osteoporosis mouse model Frontiers in Endocrinology osteoporosis metabolism LC-MS/MS lymphocyte B cell |
| title | Early changes of bone metabolites and lymphocyte subsets may participate in osteoporosis onset: a preliminary study of a postmenopausal osteoporosis mouse model |
| title_full | Early changes of bone metabolites and lymphocyte subsets may participate in osteoporosis onset: a preliminary study of a postmenopausal osteoporosis mouse model |
| title_fullStr | Early changes of bone metabolites and lymphocyte subsets may participate in osteoporosis onset: a preliminary study of a postmenopausal osteoporosis mouse model |
| title_full_unstemmed | Early changes of bone metabolites and lymphocyte subsets may participate in osteoporosis onset: a preliminary study of a postmenopausal osteoporosis mouse model |
| title_short | Early changes of bone metabolites and lymphocyte subsets may participate in osteoporosis onset: a preliminary study of a postmenopausal osteoporosis mouse model |
| title_sort | early changes of bone metabolites and lymphocyte subsets may participate in osteoporosis onset a preliminary study of a postmenopausal osteoporosis mouse model |
| topic | osteoporosis metabolism LC-MS/MS lymphocyte B cell |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2024.1323647/full |
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