Study on the changes of three main components in ganoderma lucidum spores by acid leaching and high pressure method

The effects of acid leaching high pressure method on the content of ganoderma lucidum polysaccharides, ganoderma lucidum total triterpenoids and ergosterol in unbroken ganoderma lucidum spores were compared with those of unbroken and broken ganoderma lucidum spores without being treated by acid leac...

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Main Authors: LV Si-min, HE Xiao-bing, ZHANG Li-ting, DING Hong-yan, QI Yi, WU Tie
Format: Article
Language:English
Published: Academy of National Food and Strategic Reserves Administration 2019-11-01
Series:Liang you shipin ke-ji
Subjects:
Online Access:http://lyspkj.ijournal.cn/lyspkj/article/abstract/20190617?st=article_issue
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author LV Si-min
HE Xiao-bing
ZHANG Li-ting
DING Hong-yan
QI Yi
WU Tie
author_facet LV Si-min
HE Xiao-bing
ZHANG Li-ting
DING Hong-yan
QI Yi
WU Tie
author_sort LV Si-min
collection DOAJ
description The effects of acid leaching high pressure method on the content of ganoderma lucidum polysaccharides, ganoderma lucidum total triterpenoids and ergosterol in unbroken ganoderma lucidum spores were compared with those of unbroken and broken ganoderma lucidum spores without being treated by acid leaching and high pressure.The content of ganoderma lucidum polysaccharides in ganoderma lucidum spores was determined by UV-visible spectrophotometry with anhydrous glucose as reference substance; the content of ganoderma lucidum total triterpenoids in ganoderma lucidum spores was determined by UV-visible spectrophotometry with oleanolic acid as reference substance; the content of ergosterol of Ganoderma lucidum spores was determined by reversed-phase high performance liquid chromatography with ergosterol as a reference substance, and the contents of the three components were compared according to the results.The results showed that for unbroken ganoderma lucidum spores treated by acid-leaching and high-pressure, its content of ganoderma lucidum polysaccharides was significantly higher than that of both unbroken and broken ganoderma lucidum spores without being treated by acid leaching and high-pressure, and the content of ganoderma lucidum total triterpenoids and ergosterol was significantly higher than that of unbroken ganoderma lucidum spores without being treated by acid leaching and high-pressure.The acid leaching high pressure method can be used as a new method for breaking the wall of ganoderma lucidum spores.The content of ganoderma lucidum polysaccharide, ganoderma lucidum total triterpenoids and ergosterol obtained by this method is significantly higher than that of the control group.
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spelling doaj.art-47aa975ecf6d45ceaf9870ccfee9ac832023-06-15T02:37:57ZengAcademy of National Food and Strategic Reserves AdministrationLiang you shipin ke-ji1007-75612019-11-01276939710.16210/j.cnki.1007-7561.2019.06.017Study on the changes of three main components in ganoderma lucidum spores by acid leaching and high pressure methodLV Si-min0HE Xiao-bing1ZHANG Li-ting2DING Hong-yan3QI Yi4WU Tie5Guangdong Medical University Guangdong Runhe Biological Technology Co., Ltd.Coenzyme Q10 Research Institute, Dongguan Guangdong 523808Guangdong Medical University Guangdong Runhe Biological Technology Co., Ltd.Coenzyme Q10 Research Institute, Dongguan Guangdong 523808Guangdong Medical University Guangdong Runhe Biological Technology Co., Ltd.Coenzyme Q10 Research Institute, Dongguan Guangdong 523808Guangdong Medical University Guangdong Runhe Biological Technology Co., Ltd.Coenzyme Q10 Research Institute, Dongguan Guangdong 523808Guangdong Medical University, Dongguan Guangdong 523808Guangdong Medical University, Dongguan Guangdong 523808The effects of acid leaching high pressure method on the content of ganoderma lucidum polysaccharides, ganoderma lucidum total triterpenoids and ergosterol in unbroken ganoderma lucidum spores were compared with those of unbroken and broken ganoderma lucidum spores without being treated by acid leaching and high pressure.The content of ganoderma lucidum polysaccharides in ganoderma lucidum spores was determined by UV-visible spectrophotometry with anhydrous glucose as reference substance; the content of ganoderma lucidum total triterpenoids in ganoderma lucidum spores was determined by UV-visible spectrophotometry with oleanolic acid as reference substance; the content of ergosterol of Ganoderma lucidum spores was determined by reversed-phase high performance liquid chromatography with ergosterol as a reference substance, and the contents of the three components were compared according to the results.The results showed that for unbroken ganoderma lucidum spores treated by acid-leaching and high-pressure, its content of ganoderma lucidum polysaccharides was significantly higher than that of both unbroken and broken ganoderma lucidum spores without being treated by acid leaching and high-pressure, and the content of ganoderma lucidum total triterpenoids and ergosterol was significantly higher than that of unbroken ganoderma lucidum spores without being treated by acid leaching and high-pressure.The acid leaching high pressure method can be used as a new method for breaking the wall of ganoderma lucidum spores.The content of ganoderma lucidum polysaccharide, ganoderma lucidum total triterpenoids and ergosterol obtained by this method is significantly higher than that of the control group.http://lyspkj.ijournal.cn/lyspkj/article/abstract/20190617?st=article_issueacid leachinghigh pressurewall-breakingganoderma lucidum spore
spellingShingle LV Si-min
HE Xiao-bing
ZHANG Li-ting
DING Hong-yan
QI Yi
WU Tie
Study on the changes of three main components in ganoderma lucidum spores by acid leaching and high pressure method
Liang you shipin ke-ji
acid leaching
high pressure
wall-breaking
ganoderma lucidum spore
title Study on the changes of three main components in ganoderma lucidum spores by acid leaching and high pressure method
title_full Study on the changes of three main components in ganoderma lucidum spores by acid leaching and high pressure method
title_fullStr Study on the changes of three main components in ganoderma lucidum spores by acid leaching and high pressure method
title_full_unstemmed Study on the changes of three main components in ganoderma lucidum spores by acid leaching and high pressure method
title_short Study on the changes of three main components in ganoderma lucidum spores by acid leaching and high pressure method
title_sort study on the changes of three main components in ganoderma lucidum spores by acid leaching and high pressure method
topic acid leaching
high pressure
wall-breaking
ganoderma lucidum spore
url http://lyspkj.ijournal.cn/lyspkj/article/abstract/20190617?st=article_issue
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