Genetic Diversity Assessment in Several Barley (Hordeum vulgare L.) Cultivars Using Microsatellite Markers
In the present study, genetic diversity in seven cultivars of cultivated barley (Hordeum vulgare) populations was evaluated using 10 microsatellite markers. Genomic DNA was extracted from fresh leaves and amplification reactions were done by PCR. The amplification products were separated on 6% denat...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Society of Land Measurements and Cadastre from Transylvania (SMTCT)
2011-05-01
|
| Series: | Notulae Scientia Biologicae |
| Online Access: | http://www.notulaebiologicae.ro/index.php/nsb/article/view/6041 |
| _version_ | 1818472269548093440 |
|---|---|
| author | Mohammad Reza BLORI-MOGHADAM Abbas SAFARNEJAD S. Kamal KAZEMITABAR |
| author_facet | Mohammad Reza BLORI-MOGHADAM Abbas SAFARNEJAD S. Kamal KAZEMITABAR |
| author_sort | Mohammad Reza BLORI-MOGHADAM |
| collection | DOAJ |
| description | In the present study, genetic diversity in seven cultivars of cultivated barley (Hordeum vulgare) populations was evaluated using 10 microsatellite markers. Genomic DNA was extracted from fresh leaves and amplification reactions were done by PCR. The amplification products were separated on 6% denaturing polyacrylamide gels containing 7M urea and visualized via silver staining method. High level of polymorphism was observed among populations. Polymorphic bands ranged from 100 to 300 bp. Altogether 65 alleles were observed among all genotypes, with an average of 9.2 alleles per locus for all loci. Polymorphic information content (PIC) ranged from 0.80 to 0.88 with an average of 0.84. Sahand populations showed the lowest mean of gene diversity whereas the highest mean of heterozygosity observed in Rayhan populations that can prepare a powerful resource of genetic diversity for breeding programs. The genotypes were clustered using unweight pair-group method on arithmetic average by POPGEN32 software. The dendrogram discriminated all the genotypes in several groups. The results showed that SSR markers have a high ability to reveal most of the information in a single locus and can be used for genetic analysis in molecular levels determination of genetic similarity and clustering barley cultivars. |
| first_indexed | 2024-04-14T04:04:38Z |
| format | Article |
| id | doaj.art-47ace5e675c0417895beb94c6683127a |
| institution | Directory Open Access Journal |
| issn | 2067-3205 2067-3264 |
| language | English |
| last_indexed | 2024-04-14T04:04:38Z |
| publishDate | 2011-05-01 |
| publisher | Society of Land Measurements and Cadastre from Transylvania (SMTCT) |
| record_format | Article |
| series | Notulae Scientia Biologicae |
| spelling | doaj.art-47ace5e675c0417895beb94c6683127a2022-12-22T02:13:23ZengSociety of Land Measurements and Cadastre from Transylvania (SMTCT)Notulae Scientia Biologicae2067-32052067-32642011-05-013214014410.15835/nsb3260415912Genetic Diversity Assessment in Several Barley (Hordeum vulgare L.) Cultivars Using Microsatellite MarkersMohammad Reza BLORI-MOGHADAM0Abbas SAFARNEJAD1S. Kamal KAZEMITABAR2Department of Agronomy, Plant breeding and Biotechnology, Faculty of Crop Science, Sari Agricultural Science and Natural Resources University, SariRazavi Khorasan Agricultural and Natural Resources Research Center. PO. Box. 91735-1148. MashhadDepartment of Agronomy, Plant breeding and Biotechnology, Faculty of Crop Science, Sari Agricultural Science and Natural Resources University, SariIn the present study, genetic diversity in seven cultivars of cultivated barley (Hordeum vulgare) populations was evaluated using 10 microsatellite markers. Genomic DNA was extracted from fresh leaves and amplification reactions were done by PCR. The amplification products were separated on 6% denaturing polyacrylamide gels containing 7M urea and visualized via silver staining method. High level of polymorphism was observed among populations. Polymorphic bands ranged from 100 to 300 bp. Altogether 65 alleles were observed among all genotypes, with an average of 9.2 alleles per locus for all loci. Polymorphic information content (PIC) ranged from 0.80 to 0.88 with an average of 0.84. Sahand populations showed the lowest mean of gene diversity whereas the highest mean of heterozygosity observed in Rayhan populations that can prepare a powerful resource of genetic diversity for breeding programs. The genotypes were clustered using unweight pair-group method on arithmetic average by POPGEN32 software. The dendrogram discriminated all the genotypes in several groups. The results showed that SSR markers have a high ability to reveal most of the information in a single locus and can be used for genetic analysis in molecular levels determination of genetic similarity and clustering barley cultivars.http://www.notulaebiologicae.ro/index.php/nsb/article/view/6041 |
| spellingShingle | Mohammad Reza BLORI-MOGHADAM Abbas SAFARNEJAD S. Kamal KAZEMITABAR Genetic Diversity Assessment in Several Barley (Hordeum vulgare L.) Cultivars Using Microsatellite Markers Notulae Scientia Biologicae |
| title | Genetic Diversity Assessment in Several Barley (Hordeum vulgare L.) Cultivars Using Microsatellite Markers |
| title_full | Genetic Diversity Assessment in Several Barley (Hordeum vulgare L.) Cultivars Using Microsatellite Markers |
| title_fullStr | Genetic Diversity Assessment in Several Barley (Hordeum vulgare L.) Cultivars Using Microsatellite Markers |
| title_full_unstemmed | Genetic Diversity Assessment in Several Barley (Hordeum vulgare L.) Cultivars Using Microsatellite Markers |
| title_short | Genetic Diversity Assessment in Several Barley (Hordeum vulgare L.) Cultivars Using Microsatellite Markers |
| title_sort | genetic diversity assessment in several barley hordeum vulgare l cultivars using microsatellite markers |
| url | http://www.notulaebiologicae.ro/index.php/nsb/article/view/6041 |
| work_keys_str_mv | AT mohammadrezablorimoghadam geneticdiversityassessmentinseveralbarleyhordeumvulgarelcultivarsusingmicrosatellitemarkers AT abbassafarnejad geneticdiversityassessmentinseveralbarleyhordeumvulgarelcultivarsusingmicrosatellitemarkers AT skamalkazemitabar geneticdiversityassessmentinseveralbarleyhordeumvulgarelcultivarsusingmicrosatellitemarkers |