m6A-related lncRNAs are potential biomarkers for the prognosis of COAD patients
BackgroundColon adenocarcinoma (COAD) is the most common subtype of colon cancer. However, the 5-year survival rate of COAD patients remains unsatisfactory. N6-methyladenosine (m6A) and long noncoding RNAs (lncRNAs) play essential roles in the occurrence and development of COAD. Herein, we are commi...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2022.920023/full |
| _version_ | 1811214389915680768 |
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| author | Chenyang Xu Tingting He Xinxin Shao Ling Gao Lei Cao |
| author_facet | Chenyang Xu Tingting He Xinxin Shao Ling Gao Lei Cao |
| author_sort | Chenyang Xu |
| collection | DOAJ |
| description | BackgroundColon adenocarcinoma (COAD) is the most common subtype of colon cancer. However, the 5-year survival rate of COAD patients remains unsatisfactory. N6-methyladenosine (m6A) and long noncoding RNAs (lncRNAs) play essential roles in the occurrence and development of COAD. Herein, we are committed to establish and validate a prognostic m6A-related lncRNA signature.MethodsWe obtained m6A-related lncRNAs by coexpression. The m6A-related lncRNA risk signature (m6ALncSig) was developed via univariate, LASSO, and multivariate Cox regression analyses. Kaplan-Meier (KM) survival curves, gene set enrichment analysis (GSEA), and nomogram generation were conducted to assess m6ALncSig. In addition, the potential immunotherapeutic signatures were also discussed. Real-time PCR and CCK8 analysis were performed to evaluate the expression and functions of lncRNA UBA6-AS1, which was selected.ResultsThe risk signature comprising 14 m6A-related lncRNAs (m6ALncSig) was established, which possessed a superior predictive ability of prognosis. Meanwhile, m6ALncSig was linked to immune cell infiltration. The level of UBA6-AS1 expression was validated in 17 pairs of COAD samples. In cell function experiments, UBA6-AS1 knockdown attenuated cell proliferation capacity.ConclusionsCollectively, m6ALncSig could serve as an independent predictive factor for COAD and accurately estimate the outcome for COAD patients. Importantly, UBA6-AS1 was first identified as an oncogene in COAD. |
| first_indexed | 2024-04-12T06:03:49Z |
| format | Article |
| id | doaj.art-47ae98aea8844949b6a3a4078ffae297 |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-04-12T06:03:49Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-47ae98aea8844949b6a3a4078ffae2972022-12-22T03:44:57ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2022-08-011210.3389/fonc.2022.920023920023m6A-related lncRNAs are potential biomarkers for the prognosis of COAD patientsChenyang Xu0Tingting He1Xinxin Shao2Ling Gao3Lei Cao4Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, ChinaDepartment of Interventional Radiology, The First Affiliated Hospital of Soochow University, Suzhou, ChinaJiangsu Key Laboratory of Clinical Immunology, Jiangsu Institute of Clinical Immunology, The First Affiliated Hospital of Soochow University, Suzhou, ChinaDepartment of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, ChinaJiangsu Key Laboratory of Clinical Immunology, Jiangsu Institute of Clinical Immunology, The First Affiliated Hospital of Soochow University, Suzhou, ChinaBackgroundColon adenocarcinoma (COAD) is the most common subtype of colon cancer. However, the 5-year survival rate of COAD patients remains unsatisfactory. N6-methyladenosine (m6A) and long noncoding RNAs (lncRNAs) play essential roles in the occurrence and development of COAD. Herein, we are committed to establish and validate a prognostic m6A-related lncRNA signature.MethodsWe obtained m6A-related lncRNAs by coexpression. The m6A-related lncRNA risk signature (m6ALncSig) was developed via univariate, LASSO, and multivariate Cox regression analyses. Kaplan-Meier (KM) survival curves, gene set enrichment analysis (GSEA), and nomogram generation were conducted to assess m6ALncSig. In addition, the potential immunotherapeutic signatures were also discussed. Real-time PCR and CCK8 analysis were performed to evaluate the expression and functions of lncRNA UBA6-AS1, which was selected.ResultsThe risk signature comprising 14 m6A-related lncRNAs (m6ALncSig) was established, which possessed a superior predictive ability of prognosis. Meanwhile, m6ALncSig was linked to immune cell infiltration. The level of UBA6-AS1 expression was validated in 17 pairs of COAD samples. In cell function experiments, UBA6-AS1 knockdown attenuated cell proliferation capacity.ConclusionsCollectively, m6ALncSig could serve as an independent predictive factor for COAD and accurately estimate the outcome for COAD patients. Importantly, UBA6-AS1 was first identified as an oncogene in COAD.https://www.frontiersin.org/articles/10.3389/fonc.2022.920023/fullm6A modificationcolon adenocarcinomalncRNAcell function assaysbiomarker |
| spellingShingle | Chenyang Xu Tingting He Xinxin Shao Ling Gao Lei Cao m6A-related lncRNAs are potential biomarkers for the prognosis of COAD patients Frontiers in Oncology m6A modification colon adenocarcinoma lncRNA cell function assays biomarker |
| title | m6A-related lncRNAs are potential biomarkers for the prognosis of COAD patients |
| title_full | m6A-related lncRNAs are potential biomarkers for the prognosis of COAD patients |
| title_fullStr | m6A-related lncRNAs are potential biomarkers for the prognosis of COAD patients |
| title_full_unstemmed | m6A-related lncRNAs are potential biomarkers for the prognosis of COAD patients |
| title_short | m6A-related lncRNAs are potential biomarkers for the prognosis of COAD patients |
| title_sort | m6a related lncrnas are potential biomarkers for the prognosis of coad patients |
| topic | m6A modification colon adenocarcinoma lncRNA cell function assays biomarker |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2022.920023/full |
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