Bavachin from Psoralea corylifolia Improves Insulin-Dependent Glucose Uptake through Insulin Signaling and AMPK Activation in 3T3-L1 Adipocytes
The fruit of Psoralea corylifolia L. (Fabaceae) (PC), known as “Bo-Gol-Zhee” in Korea has been used as traditional medicine. Ethanol and aqueous extracts of PC have an anti-hyperglycemic effect by increasing plasma insulin levels and decreasing blood glucose and total plasma cholesterol levels in ty...
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MDPI AG
2016-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | http://www.mdpi.com/1422-0067/17/4/527 |
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| author | Hyejin Lee Hua Li Minsoo Noh Jae-Ha Ryu |
| author_facet | Hyejin Lee Hua Li Minsoo Noh Jae-Ha Ryu |
| author_sort | Hyejin Lee |
| collection | DOAJ |
| description | The fruit of Psoralea corylifolia L. (Fabaceae) (PC), known as “Bo-Gol-Zhee” in Korea has been used as traditional medicine. Ethanol and aqueous extracts of PC have an anti-hyperglycemic effect by increasing plasma insulin levels and decreasing blood glucose and total plasma cholesterol levels in type 2 diabetic rats. In this study, we purified six compounds from PC and investigated their anti-diabetic effect. Among the purified compounds, bavachin most potently accumulated lipids during adipocyte differentiation. Intracellular lipid accumulation was measured by Oil Red-O (ORO) cell staining to investigate the effect of compounds on adipogenesis. Consistently, bavachin activated gene expression of adipogenic transcriptional factors, proliferator-activated receptorγ (PPARγ) and CCAAT/enhancer binding protein-α (C/EBPα). Bavachin also increased adiponectin expression and secretion in adipocytes. Moreover, bavachin increased insulin-induced glucose uptake by differentiated adipocytes and myoblasts. In differentiated adipocytes, we found that bavachin enhanced glucose uptake via glucose transporter 4 (GLUT4) translocation by activating the Akt and 5′AMP-activated protein kinase (AMPK) pathway in the presence or absence of insulin. These results suggest that bavachin from Psoralea corylifolia might have therapeutic potential for type 2 diabetes by activating insulin signaling pathways. |
| first_indexed | 2024-04-12T13:43:39Z |
| format | Article |
| id | doaj.art-47af0407de0447d2ab9bf07be3df9fb9 |
| institution | Directory Open Access Journal |
| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-12T13:43:39Z |
| publishDate | 2016-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-47af0407de0447d2ab9bf07be3df9fb92022-12-22T03:30:45ZengMDPI AGInternational Journal of Molecular Sciences1422-00672016-04-0117452710.3390/ijms17040527ijms17040527Bavachin from Psoralea corylifolia Improves Insulin-Dependent Glucose Uptake through Insulin Signaling and AMPK Activation in 3T3-L1 AdipocytesHyejin Lee0Hua Li1Minsoo Noh2Jae-Ha Ryu3College of Pharmacy and Research Center for Cell Fate Control, Sookmyung Women’s University, 52 Hyochangwongil, Yongsan-gu, Seoul 140-742, KoreaCollege of Pharmacy and Research Center for Cell Fate Control, Sookmyung Women’s University, 52 Hyochangwongil, Yongsan-gu, Seoul 140-742, KoreaNatural Products Research Institute, College of Pharmacy, Seoul National University, 1 Gwanak-ro, Gwanak-Gu, Seoul 151-742, KoreaCollege of Pharmacy and Research Center for Cell Fate Control, Sookmyung Women’s University, 52 Hyochangwongil, Yongsan-gu, Seoul 140-742, KoreaThe fruit of Psoralea corylifolia L. (Fabaceae) (PC), known as “Bo-Gol-Zhee” in Korea has been used as traditional medicine. Ethanol and aqueous extracts of PC have an anti-hyperglycemic effect by increasing plasma insulin levels and decreasing blood glucose and total plasma cholesterol levels in type 2 diabetic rats. In this study, we purified six compounds from PC and investigated their anti-diabetic effect. Among the purified compounds, bavachin most potently accumulated lipids during adipocyte differentiation. Intracellular lipid accumulation was measured by Oil Red-O (ORO) cell staining to investigate the effect of compounds on adipogenesis. Consistently, bavachin activated gene expression of adipogenic transcriptional factors, proliferator-activated receptorγ (PPARγ) and CCAAT/enhancer binding protein-α (C/EBPα). Bavachin also increased adiponectin expression and secretion in adipocytes. Moreover, bavachin increased insulin-induced glucose uptake by differentiated adipocytes and myoblasts. In differentiated adipocytes, we found that bavachin enhanced glucose uptake via glucose transporter 4 (GLUT4) translocation by activating the Akt and 5′AMP-activated protein kinase (AMPK) pathway in the presence or absence of insulin. These results suggest that bavachin from Psoralea corylifolia might have therapeutic potential for type 2 diabetes by activating insulin signaling pathways.http://www.mdpi.com/1422-0067/17/4/527diabetes mellitusglucose uptakePsoralea corylifolia3T3-L1adipogenesis |
| spellingShingle | Hyejin Lee Hua Li Minsoo Noh Jae-Ha Ryu Bavachin from Psoralea corylifolia Improves Insulin-Dependent Glucose Uptake through Insulin Signaling and AMPK Activation in 3T3-L1 Adipocytes International Journal of Molecular Sciences diabetes mellitus glucose uptake Psoralea corylifolia 3T3-L1 adipogenesis |
| title | Bavachin from Psoralea corylifolia Improves Insulin-Dependent Glucose Uptake through Insulin Signaling and AMPK Activation in 3T3-L1 Adipocytes |
| title_full | Bavachin from Psoralea corylifolia Improves Insulin-Dependent Glucose Uptake through Insulin Signaling and AMPK Activation in 3T3-L1 Adipocytes |
| title_fullStr | Bavachin from Psoralea corylifolia Improves Insulin-Dependent Glucose Uptake through Insulin Signaling and AMPK Activation in 3T3-L1 Adipocytes |
| title_full_unstemmed | Bavachin from Psoralea corylifolia Improves Insulin-Dependent Glucose Uptake through Insulin Signaling and AMPK Activation in 3T3-L1 Adipocytes |
| title_short | Bavachin from Psoralea corylifolia Improves Insulin-Dependent Glucose Uptake through Insulin Signaling and AMPK Activation in 3T3-L1 Adipocytes |
| title_sort | bavachin from psoralea corylifolia improves insulin dependent glucose uptake through insulin signaling and ampk activation in 3t3 l1 adipocytes |
| topic | diabetes mellitus glucose uptake Psoralea corylifolia 3T3-L1 adipogenesis |
| url | http://www.mdpi.com/1422-0067/17/4/527 |
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