Antimalarial Activity of Solvent Fractions of the Stem Bark of Periploca linearifolia Quart.-Dill. & A.Rich. (Asclepiadaceae) Against Plasmodium berghei in Mice
Wubetu Yihunie Belay,1 Abyot Endale Gurmu,2 Zewdu Birhanu Wubneh3 1Department of Pharmacy, College of Health Sciences, Debre Markos University, Debre Markos, Ethiopia; 2Department of Pharmacognosy, School of Pharmacy, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia; 3...
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Dove Medical Press
2022-01-01
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| Series: | Journal of Experimental Pharmacology |
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| Online Access: | https://www.dovepress.com/antimalarial-activity-of-solvent-fractions-of-the-stem-bark-of-periplo-peer-reviewed-fulltext-article-JEP |
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| author | Belay WY Gurmu AE Wubneh ZB |
| author_facet | Belay WY Gurmu AE Wubneh ZB |
| author_sort | Belay WY |
| collection | DOAJ |
| description | Wubetu Yihunie Belay,1 Abyot Endale Gurmu,2 Zewdu Birhanu Wubneh3 1Department of Pharmacy, College of Health Sciences, Debre Markos University, Debre Markos, Ethiopia; 2Department of Pharmacognosy, School of Pharmacy, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia; 3Department of Pharmacology, School of Pharmacy, College of Medicine and Health Sciences, University of Gondar, Gondar, EthiopiaCorrespondence: Wubetu Yihunie BelayDepartment of Pharmacy, College of Health Sciences, Debre Markos University Tel +251910112391Email wubetuyihunie@gmail.comBackground: Currently, malaria elimination is intimidated by the emergence of antimalarial drug and insecticide resistance. This scenario urges scientists to develop new antimalarial drugs, where plant-based compounds are the major candidates.Objective: The objective of this experiment was to examine the antimalarial activities of the solvent fractions of the stem bark of P. linearifolia in mice.Methods: The dried hydromethanolic extract was treated with n-hexane, chloroform, and ethyl acetate solvents based on increasing order of polarity. For the suppressive test, after three hours post-inoculation of P. berghei, the first group was administered the vehicle 2 mL/100 g. The second group was given chloroquine 25 mg/kg, whereas the remaining nine groups were administered 100, 200, and 400 mg/kg of each of the chloroform, ethyl acetate and aqueous fractions. Furthermore, the aqueous fraction was evaluated using Rane’s model for the curative test. The result was statistically analyzed using a one-way analysis of variance (ANOVA). The results were considered significant at P < 0.05.Results: In the suppressive test, the solvent fractions suppressed parasitemia level significantly (P < 0.01 for 100 mg/kg, P < 0.001 for 200 and 400 mg/kg of the aqueous and P < 0.01 for 200 mg/kg, P < 0.001 for 400 mg/kg of chloroform fractions) as compared to vehicle-treated mice, whereas in the curative model 400 mg/kg of the aqueous fraction (P < 0.01) showed a significant parasitemia level reduction.Conclusion: The results of this study indicated that the solvent fractions have promising antiplasmodial activity against Plasmodium berghei, which is consistent with the earlier in vivo study of the hydromethanolic extract.Keywords: antimalarial, Plasmodium berghei, Periploca linearifolia, solvent fractions |
| first_indexed | 2024-04-11T17:20:00Z |
| format | Article |
| id | doaj.art-47af64286bf14973b1363d7aa68ef7b3 |
| institution | Directory Open Access Journal |
| issn | 1179-1454 |
| language | English |
| last_indexed | 2024-04-11T17:20:00Z |
| publishDate | 2022-01-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Journal of Experimental Pharmacology |
| spelling | doaj.art-47af64286bf14973b1363d7aa68ef7b32022-12-22T04:12:30ZengDove Medical PressJournal of Experimental Pharmacology1179-14542022-01-01Volume 1471572236Antimalarial Activity of Solvent Fractions of the Stem Bark of Periploca linearifolia Quart.-Dill. & A.Rich. (Asclepiadaceae) Against Plasmodium berghei in MiceBelay WYGurmu AEWubneh ZBWubetu Yihunie Belay,1 Abyot Endale Gurmu,2 Zewdu Birhanu Wubneh3 1Department of Pharmacy, College of Health Sciences, Debre Markos University, Debre Markos, Ethiopia; 2Department of Pharmacognosy, School of Pharmacy, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia; 3Department of Pharmacology, School of Pharmacy, College of Medicine and Health Sciences, University of Gondar, Gondar, EthiopiaCorrespondence: Wubetu Yihunie BelayDepartment of Pharmacy, College of Health Sciences, Debre Markos University Tel +251910112391Email wubetuyihunie@gmail.comBackground: Currently, malaria elimination is intimidated by the emergence of antimalarial drug and insecticide resistance. This scenario urges scientists to develop new antimalarial drugs, where plant-based compounds are the major candidates.Objective: The objective of this experiment was to examine the antimalarial activities of the solvent fractions of the stem bark of P. linearifolia in mice.Methods: The dried hydromethanolic extract was treated with n-hexane, chloroform, and ethyl acetate solvents based on increasing order of polarity. For the suppressive test, after three hours post-inoculation of P. berghei, the first group was administered the vehicle 2 mL/100 g. The second group was given chloroquine 25 mg/kg, whereas the remaining nine groups were administered 100, 200, and 400 mg/kg of each of the chloroform, ethyl acetate and aqueous fractions. Furthermore, the aqueous fraction was evaluated using Rane’s model for the curative test. The result was statistically analyzed using a one-way analysis of variance (ANOVA). The results were considered significant at P < 0.05.Results: In the suppressive test, the solvent fractions suppressed parasitemia level significantly (P < 0.01 for 100 mg/kg, P < 0.001 for 200 and 400 mg/kg of the aqueous and P < 0.01 for 200 mg/kg, P < 0.001 for 400 mg/kg of chloroform fractions) as compared to vehicle-treated mice, whereas in the curative model 400 mg/kg of the aqueous fraction (P < 0.01) showed a significant parasitemia level reduction.Conclusion: The results of this study indicated that the solvent fractions have promising antiplasmodial activity against Plasmodium berghei, which is consistent with the earlier in vivo study of the hydromethanolic extract.Keywords: antimalarial, Plasmodium berghei, Periploca linearifolia, solvent fractionshttps://www.dovepress.com/antimalarial-activity-of-solvent-fractions-of-the-stem-bark-of-periplo-peer-reviewed-fulltext-article-JEPantimalarialplasmodium bergheiperiploca linearifoliasolvent fractions |
| spellingShingle | Belay WY Gurmu AE Wubneh ZB Antimalarial Activity of Solvent Fractions of the Stem Bark of Periploca linearifolia Quart.-Dill. & A.Rich. (Asclepiadaceae) Against Plasmodium berghei in Mice Journal of Experimental Pharmacology antimalarial plasmodium berghei periploca linearifolia solvent fractions |
| title | Antimalarial Activity of Solvent Fractions of the Stem Bark of Periploca linearifolia Quart.-Dill. & A.Rich. (Asclepiadaceae) Against Plasmodium berghei in Mice |
| title_full | Antimalarial Activity of Solvent Fractions of the Stem Bark of Periploca linearifolia Quart.-Dill. & A.Rich. (Asclepiadaceae) Against Plasmodium berghei in Mice |
| title_fullStr | Antimalarial Activity of Solvent Fractions of the Stem Bark of Periploca linearifolia Quart.-Dill. & A.Rich. (Asclepiadaceae) Against Plasmodium berghei in Mice |
| title_full_unstemmed | Antimalarial Activity of Solvent Fractions of the Stem Bark of Periploca linearifolia Quart.-Dill. & A.Rich. (Asclepiadaceae) Against Plasmodium berghei in Mice |
| title_short | Antimalarial Activity of Solvent Fractions of the Stem Bark of Periploca linearifolia Quart.-Dill. & A.Rich. (Asclepiadaceae) Against Plasmodium berghei in Mice |
| title_sort | antimalarial activity of solvent fractions of the stem bark of periploca linearifolia quart dill amp a rich asclepiadaceae against plasmodium berghei in mice |
| topic | antimalarial plasmodium berghei periploca linearifolia solvent fractions |
| url | https://www.dovepress.com/antimalarial-activity-of-solvent-fractions-of-the-stem-bark-of-periplo-peer-reviewed-fulltext-article-JEP |
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