Synthesis and evaluation of polymeric micelle containing piperacillin/tazobactam for enhanced antibacterial activity
Infections caused by multidrug-resistant bacteria such as P. aeruginosa are important therapeutic complications. Piperacillin/Tazobactam is considered a safe antimicrobial agent. But we should not ignore the prevalence of resistant strains to this drug. In this work, a new polymeric micelle composed...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-01-01
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| Series: | Drug Delivery |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/10717544.2019.1693708 |
| _version_ | 1828802230451437568 |
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| author | Milani Morteza Salehi Roya Hamishehkar Hamed Zarebkohan Amir Akbarzadeh Abolfazl |
| author_facet | Milani Morteza Salehi Roya Hamishehkar Hamed Zarebkohan Amir Akbarzadeh Abolfazl |
| author_sort | Milani Morteza |
| collection | DOAJ |
| description | Infections caused by multidrug-resistant bacteria such as P. aeruginosa are important therapeutic complications. Piperacillin/Tazobactam is considered a safe antimicrobial agent. But we should not ignore the prevalence of resistant strains to this drug. In this work, a new polymeric micelle composed of Piperacillin/Tazobactam-loaded Poly (ethylene glycol) methyl ether-block-poly (lactide-co-glycolide) (PLGA-PEG) was developed to improve the antimicrobial performance of P/T. The SEM and TEM studies of PLGA-PEG micelle showed, semi-spherical morphology with a mean diameter of below 30 nm. Zeta potential results indicated that the surface charge of PLGA-PEG micelle was −2.98 mV, while after encapsulation of P/T, the surface charge decreases to −4.13 mV. Clinical strains of P. aeruginosa were isolated and their resistance pattern against different antibiotics was evaluated. The MIC of free and P/T -Loaded PLGA-PEG micelles was determined. Also, the effect of free or P/T micelle against minimal biofilm eradication concentration and motility inhibition was evaluated. The bacterial isolates were resistant to most common antibiotics. The MIC of the free drug form and micelle form ranged from 4 to 512 µg/ml and 2 to 256 µg/ml, respectively. Generally, micelle showed more effective antibiofilm activities, inhibition of bacterial motility and reducing the MIC than that free drug form. |
| first_indexed | 2024-12-12T07:00:23Z |
| format | Article |
| id | doaj.art-47afe50ab7b549df9217e35838486f73 |
| institution | Directory Open Access Journal |
| issn | 1071-7544 1521-0464 |
| language | English |
| last_indexed | 2024-12-12T07:00:23Z |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Drug Delivery |
| spelling | doaj.art-47afe50ab7b549df9217e35838486f732022-12-22T00:33:51ZengTaylor & Francis GroupDrug Delivery1071-75441521-04642019-01-012611292129910.1080/10717544.2019.16937081693708Synthesis and evaluation of polymeric micelle containing piperacillin/tazobactam for enhanced antibacterial activityMilani Morteza0Salehi Roya1Hamishehkar Hamed2Zarebkohan Amir3Akbarzadeh Abolfazl4Tabriz University of Medical SciencesTabriz University of Medical SciencesTabriz University of Medical SciencesTabriz University of Medical SciencesTabriz University of Medical SciencesInfections caused by multidrug-resistant bacteria such as P. aeruginosa are important therapeutic complications. Piperacillin/Tazobactam is considered a safe antimicrobial agent. But we should not ignore the prevalence of resistant strains to this drug. In this work, a new polymeric micelle composed of Piperacillin/Tazobactam-loaded Poly (ethylene glycol) methyl ether-block-poly (lactide-co-glycolide) (PLGA-PEG) was developed to improve the antimicrobial performance of P/T. The SEM and TEM studies of PLGA-PEG micelle showed, semi-spherical morphology with a mean diameter of below 30 nm. Zeta potential results indicated that the surface charge of PLGA-PEG micelle was −2.98 mV, while after encapsulation of P/T, the surface charge decreases to −4.13 mV. Clinical strains of P. aeruginosa were isolated and their resistance pattern against different antibiotics was evaluated. The MIC of free and P/T -Loaded PLGA-PEG micelles was determined. Also, the effect of free or P/T micelle against minimal biofilm eradication concentration and motility inhibition was evaluated. The bacterial isolates were resistant to most common antibiotics. The MIC of the free drug form and micelle form ranged from 4 to 512 µg/ml and 2 to 256 µg/ml, respectively. Generally, micelle showed more effective antibiofilm activities, inhibition of bacterial motility and reducing the MIC than that free drug form.http://dx.doi.org/10.1080/10717544.2019.1693708p. aeruginosapiperacillin/tazobactampolymeric micelleantibiotic resistance |
| spellingShingle | Milani Morteza Salehi Roya Hamishehkar Hamed Zarebkohan Amir Akbarzadeh Abolfazl Synthesis and evaluation of polymeric micelle containing piperacillin/tazobactam for enhanced antibacterial activity Drug Delivery p. aeruginosa piperacillin/tazobactam polymeric micelle antibiotic resistance |
| title | Synthesis and evaluation of polymeric micelle containing piperacillin/tazobactam for enhanced antibacterial activity |
| title_full | Synthesis and evaluation of polymeric micelle containing piperacillin/tazobactam for enhanced antibacterial activity |
| title_fullStr | Synthesis and evaluation of polymeric micelle containing piperacillin/tazobactam for enhanced antibacterial activity |
| title_full_unstemmed | Synthesis and evaluation of polymeric micelle containing piperacillin/tazobactam for enhanced antibacterial activity |
| title_short | Synthesis and evaluation of polymeric micelle containing piperacillin/tazobactam for enhanced antibacterial activity |
| title_sort | synthesis and evaluation of polymeric micelle containing piperacillin tazobactam for enhanced antibacterial activity |
| topic | p. aeruginosa piperacillin/tazobactam polymeric micelle antibiotic resistance |
| url | http://dx.doi.org/10.1080/10717544.2019.1693708 |
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