Immune Responses Raised in an Experimental Colon Carcinoma Model Following Oral Administration of <i>Lactobacillus casei</i>
The role of dietary probiotic strains on host anti-cancer immune responses against experimental colon carcinoma was investigated. We have previously shown that <i>Lactobacillus casei</i> administration led to tumor growth suppression in an experimental colon cancer model. Here, we invest...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-02-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/12/2/368 |
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| author | Georgios Aindelis Angeliki Tiptiri-Kourpeti Evangeli Lampri Katerina Spyridopoulou Eleftheria Lamprianidou Ioannis Kotsianidis Petros Ypsilantis Aglaia Pappa Katerina Chlichlia |
| author_facet | Georgios Aindelis Angeliki Tiptiri-Kourpeti Evangeli Lampri Katerina Spyridopoulou Eleftheria Lamprianidou Ioannis Kotsianidis Petros Ypsilantis Aglaia Pappa Katerina Chlichlia |
| author_sort | Georgios Aindelis |
| collection | DOAJ |
| description | The role of dietary probiotic strains on host anti-cancer immune responses against experimental colon carcinoma was investigated. We have previously shown that <i>Lactobacillus casei</i> administration led to tumor growth suppression in an experimental colon cancer model. Here, we investigated the underlying immune mechanisms involved in this tumor-growth inhibitory effect. BALB/c mice received daily live lactobacilli <i>per os</i> prior to the establishment of a syngeneic subcutaneous CT26 tumor. Tumor volume, cytokine production, T cell differentiation and migration, as well as tumor cell apoptosis were examined to outline potential immunomodulatory effects following <i>L. casei</i> oral intake. Probiotic administration in mice resulted in a significant increase in interferon gamma (IFN-γ), Granzyme B and chemokine production in the tumor tissue as well as enhanced CD8<sup>+</sup> T cell infiltration, accompanied by a suppression of tumor growth. Cytotoxic activity against cancer cells was enhanced in probiotic-fed compared to control mice, as evidenced by the elevation of apoptotic markers, such as cleaved caspase 3 and poly (ADP-ribose) polymerase 1 (PARP1), in tumor tissue. Oral administration of <i>Lactobacillus casei</i> induced potent Th1 immune responses and cytotoxic T cell infiltration in the tumor tissue of tumor-bearing mice, resulting in tumor growth inhibition. Thus, the microorganism may hold promise as a novel dietary immunoadjuvant in raising protective anti-cancer immune responses. |
| first_indexed | 2024-03-12T06:40:59Z |
| format | Article |
| id | doaj.art-47b3550ce88943d890f85cef8ce8f337 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-12T06:40:59Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-47b3550ce88943d890f85cef8ce8f3372023-09-03T01:00:47ZengMDPI AGCancers2072-66942020-02-0112236810.3390/cancers12020368cancers12020368Immune Responses Raised in an Experimental Colon Carcinoma Model Following Oral Administration of <i>Lactobacillus casei</i>Georgios Aindelis0Angeliki Tiptiri-Kourpeti1Evangeli Lampri2Katerina Spyridopoulou3Eleftheria Lamprianidou4Ioannis Kotsianidis5Petros Ypsilantis6Aglaia Pappa7Katerina Chlichlia8Department of Molecular Biology and Genetics, Democritus University of Thrace, University Campus Dragana, 68100 Alexandroupolis, GreeceDepartment of Molecular Biology and Genetics, Democritus University of Thrace, University Campus Dragana, 68100 Alexandroupolis, GreeceDepartment of Molecular Biology and Genetics, Democritus University of Thrace, University Campus Dragana, 68100 Alexandroupolis, GreeceDepartment of Molecular Biology and Genetics, Democritus University of Thrace, University Campus Dragana, 68100 Alexandroupolis, GreeceDepartment of Hematology, School of Medicine, Democritus University of Thrace, University Campus Dragana, 68100 Alexandroupolis, GreeceDepartment of Hematology, School of Medicine, Democritus University of Thrace, University Campus Dragana, 68100 Alexandroupolis, GreeceLaboratory of Experimental Surgery and Surgical Research, School of Medicine, Democritus University of Thrace, 68100 Alexandroupolis, GreeceDepartment of Molecular Biology and Genetics, Democritus University of Thrace, University Campus Dragana, 68100 Alexandroupolis, GreeceDepartment of Molecular Biology and Genetics, Democritus University of Thrace, University Campus Dragana, 68100 Alexandroupolis, GreeceThe role of dietary probiotic strains on host anti-cancer immune responses against experimental colon carcinoma was investigated. We have previously shown that <i>Lactobacillus casei</i> administration led to tumor growth suppression in an experimental colon cancer model. Here, we investigated the underlying immune mechanisms involved in this tumor-growth inhibitory effect. BALB/c mice received daily live lactobacilli <i>per os</i> prior to the establishment of a syngeneic subcutaneous CT26 tumor. Tumor volume, cytokine production, T cell differentiation and migration, as well as tumor cell apoptosis were examined to outline potential immunomodulatory effects following <i>L. casei</i> oral intake. Probiotic administration in mice resulted in a significant increase in interferon gamma (IFN-γ), Granzyme B and chemokine production in the tumor tissue as well as enhanced CD8<sup>+</sup> T cell infiltration, accompanied by a suppression of tumor growth. Cytotoxic activity against cancer cells was enhanced in probiotic-fed compared to control mice, as evidenced by the elevation of apoptotic markers, such as cleaved caspase 3 and poly (ADP-ribose) polymerase 1 (PARP1), in tumor tissue. Oral administration of <i>Lactobacillus casei</i> induced potent Th1 immune responses and cytotoxic T cell infiltration in the tumor tissue of tumor-bearing mice, resulting in tumor growth inhibition. Thus, the microorganism may hold promise as a novel dietary immunoadjuvant in raising protective anti-cancer immune responses.https://www.mdpi.com/2072-6694/12/2/368probioticssyngeneic murine colon carcinomaimmune responsesoral administration<i>lactobacillus casei</i> |
| spellingShingle | Georgios Aindelis Angeliki Tiptiri-Kourpeti Evangeli Lampri Katerina Spyridopoulou Eleftheria Lamprianidou Ioannis Kotsianidis Petros Ypsilantis Aglaia Pappa Katerina Chlichlia Immune Responses Raised in an Experimental Colon Carcinoma Model Following Oral Administration of <i>Lactobacillus casei</i> Cancers probiotics syngeneic murine colon carcinoma immune responses oral administration <i>lactobacillus casei</i> |
| title | Immune Responses Raised in an Experimental Colon Carcinoma Model Following Oral Administration of <i>Lactobacillus casei</i> |
| title_full | Immune Responses Raised in an Experimental Colon Carcinoma Model Following Oral Administration of <i>Lactobacillus casei</i> |
| title_fullStr | Immune Responses Raised in an Experimental Colon Carcinoma Model Following Oral Administration of <i>Lactobacillus casei</i> |
| title_full_unstemmed | Immune Responses Raised in an Experimental Colon Carcinoma Model Following Oral Administration of <i>Lactobacillus casei</i> |
| title_short | Immune Responses Raised in an Experimental Colon Carcinoma Model Following Oral Administration of <i>Lactobacillus casei</i> |
| title_sort | immune responses raised in an experimental colon carcinoma model following oral administration of i lactobacillus casei i |
| topic | probiotics syngeneic murine colon carcinoma immune responses oral administration <i>lactobacillus casei</i> |
| url | https://www.mdpi.com/2072-6694/12/2/368 |
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