Experimental ochratoxicosis A in Nile tilapia and its amelioration by some feed additives
Ochratoxin A (OTA) formation prevention is not usually available so counteracting strategies are urgent. This study investigated the toxic effects of OTA on Nile tilapia with trials to neutralize these effects by using some feed additives. Supplements used as one percent (Garlen Extra4, Nigella sati...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-12-01
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| Series: | International Journal of Veterinary Science and Medicine |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2314459918301480 |
| _version_ | 1818108990796595200 |
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| author | Amany M. Diab Salem R.M. El-Keredy M.S. Abeer Gehan I.E. Ali Nagwan El-Habashi |
| author_facet | Amany M. Diab Salem R.M. El-Keredy M.S. Abeer Gehan I.E. Ali Nagwan El-Habashi |
| author_sort | Amany M. Diab |
| collection | DOAJ |
| description | Ochratoxin A (OTA) formation prevention is not usually available so counteracting strategies are urgent. This study investigated the toxic effects of OTA on Nile tilapia with trials to neutralize these effects by using some feed additives. Supplements used as one percent (Garlen Extra4, Nigella sativa, Garlen Extra4 plus Nigella sativa and a commercial mycotoxins binder, Fero Bind Pro) were added to 500 ppb/kg OTA-contaminated diet. Fish were fed at 3% body weight per day for 10 weeks. The clinical signs recorded in OTA – intoxicated positive control group were sluggish swimming and off food before death with reduction in survivability (53%) and growth performance. Several post-mortem lesions were in liver, kidneys and spleen. Serum levels of ALT, AST, creatinine and urea were significantly increased with reduction in total protein, albumin and globulin in ochratoxicated fish group compared to the negative control group. Concerning the pathological changes that have been noticed in ochratoxin treated fish were almost completely alleviated in examined tissues of fish that were fed on diet with Garlen Extra4 plus Nigella sativa or Nigella sativa and partially in fish that were fed on diet with Garlen Extra4 or Fero Bind Pro, respectively. Best detoxifying results were obtained by using 30 g/kg Nigella sativa plus 0.1 g/kg Garlen Extra4 followed by Nigella sativa, Garlen Extra4 then Fero Bind Pro as a commercial Mycotoxins binder. It could be concluded that inclusions of 30 g/kg Nigella sativa plus 0.1 g/kg Garlen Extra4 in Oreochromis niloticus (O. niloticus) diets could partially reduce OTA toxic effects. Keywords: Detoxifying agents, Feed additives, Garlen, Mycotoxins binder, Nigella sativa |
| first_indexed | 2024-12-11T02:24:08Z |
| format | Article |
| id | doaj.art-47b511a6df9b44619aedb2ae1b57e1a8 |
| institution | Directory Open Access Journal |
| issn | 2314-4599 |
| language | English |
| last_indexed | 2024-12-11T02:24:08Z |
| publishDate | 2018-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | International Journal of Veterinary Science and Medicine |
| spelling | doaj.art-47b511a6df9b44619aedb2ae1b57e1a82022-12-22T01:23:58ZengTaylor & Francis GroupInternational Journal of Veterinary Science and Medicine2314-45992018-12-0162149158Experimental ochratoxicosis A in Nile tilapia and its amelioration by some feed additivesAmany M. Diab0Salem R.M.1El-Keredy M.S. Abeer2Gehan I.E. Ali3Nagwan El-Habashi4Aquatic Microbiology, Faculty of Aquatic and Fisheries Sciences, Kafrelsheikh University, Egypt; Corresponding author.Animal Health Research Institute Dokki, EgyptUnit of Biochemistry, Nutritional Deficiency Diseases and Toxicology Animal Health Research Institute Kafr El-Sheikh, EgyptUnit of Biochemistry, Nutritional Deficiency Diseases and Toxicology Animal Health Research Institute Kafr El-Sheikh, EgyptDepartment of Pathology, Faculty of Veterinary Medicine, Kafr El-Sheikh University, EgyptOchratoxin A (OTA) formation prevention is not usually available so counteracting strategies are urgent. This study investigated the toxic effects of OTA on Nile tilapia with trials to neutralize these effects by using some feed additives. Supplements used as one percent (Garlen Extra4, Nigella sativa, Garlen Extra4 plus Nigella sativa and a commercial mycotoxins binder, Fero Bind Pro) were added to 500 ppb/kg OTA-contaminated diet. Fish were fed at 3% body weight per day for 10 weeks. The clinical signs recorded in OTA – intoxicated positive control group were sluggish swimming and off food before death with reduction in survivability (53%) and growth performance. Several post-mortem lesions were in liver, kidneys and spleen. Serum levels of ALT, AST, creatinine and urea were significantly increased with reduction in total protein, albumin and globulin in ochratoxicated fish group compared to the negative control group. Concerning the pathological changes that have been noticed in ochratoxin treated fish were almost completely alleviated in examined tissues of fish that were fed on diet with Garlen Extra4 plus Nigella sativa or Nigella sativa and partially in fish that were fed on diet with Garlen Extra4 or Fero Bind Pro, respectively. Best detoxifying results were obtained by using 30 g/kg Nigella sativa plus 0.1 g/kg Garlen Extra4 followed by Nigella sativa, Garlen Extra4 then Fero Bind Pro as a commercial Mycotoxins binder. It could be concluded that inclusions of 30 g/kg Nigella sativa plus 0.1 g/kg Garlen Extra4 in Oreochromis niloticus (O. niloticus) diets could partially reduce OTA toxic effects. Keywords: Detoxifying agents, Feed additives, Garlen, Mycotoxins binder, Nigella sativahttp://www.sciencedirect.com/science/article/pii/S2314459918301480 |
| spellingShingle | Amany M. Diab Salem R.M. El-Keredy M.S. Abeer Gehan I.E. Ali Nagwan El-Habashi Experimental ochratoxicosis A in Nile tilapia and its amelioration by some feed additives International Journal of Veterinary Science and Medicine |
| title | Experimental ochratoxicosis A in Nile tilapia and its amelioration by some feed additives |
| title_full | Experimental ochratoxicosis A in Nile tilapia and its amelioration by some feed additives |
| title_fullStr | Experimental ochratoxicosis A in Nile tilapia and its amelioration by some feed additives |
| title_full_unstemmed | Experimental ochratoxicosis A in Nile tilapia and its amelioration by some feed additives |
| title_short | Experimental ochratoxicosis A in Nile tilapia and its amelioration by some feed additives |
| title_sort | experimental ochratoxicosis a in nile tilapia and its amelioration by some feed additives |
| url | http://www.sciencedirect.com/science/article/pii/S2314459918301480 |
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