Molecular detection of Staphylococcus aureus enterotoxin genes isolated from mastitic milk and humans in El-Behira, Egypt
Background and Aim: Milk is a chief source of many nutrients. However, we must also bear in mind that it is a potential source for many cases of food poisoning. This study was conducted to investigate the prevalence of cow mastitis and evaluate the presence of enterotoxins and antibiotic resistance...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Veterinary World
2021-03-01
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| Series: | International Journal of One Health |
| Subjects: | |
| Online Access: | https://www.onehealthjournal.org/Vol.7/No.1/9.pdf |
| _version_ | 1811271094708994048 |
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| author | Mohamed S. Diab Nermin A. Ibrahim Yasser F. Elnaker Sherif A. Zidan Marwa A. Saad |
| author_facet | Mohamed S. Diab Nermin A. Ibrahim Yasser F. Elnaker Sherif A. Zidan Marwa A. Saad |
| author_sort | Mohamed S. Diab |
| collection | DOAJ |
| description | Background and Aim: Milk is a chief source of many nutrients. However, we must also bear in mind that it is a potential source for many cases of food poisoning. This study was conducted to investigate the prevalence of cow mastitis and evaluate the presence of enterotoxins and antibiotic resistance patterns in Staphylococcus aureus isolated from milk and contact humans in El-Behira Province, Egypt.
Materials and Methods: A total of 680 milk samples from 170 cows and 86 human samples consisting of 43 hand swabs and 43 nasal swabs were analyzed. The milk samples were subjected to the California mastitis test.
Results: The general occurrence was 23.1% (157/680) where 48 quarters had clinical mastitis and 109 had subclinical mastitis. Subsequently, S. aureus was isolated in Baird-Parker agar where typical and atypical colonies were selected and submitted to coagulase and complementary tests. Out of 48 samples of mastitic milk studied, 16 (33.3%) showed contamination by S. aureus whereas 109 samples of subclinical mastitis showed contamination in only 18 (16.5%). On the opposite hand, of the 86 human samples, 33 revealed S. aureus contamination, corresponding to 38.37% of the samples. Furthermore, multiplex polymerase chain reaction targeting nuc and the staphylococcal enterotoxin-encoding genes sea, seb, sec, sed, and see were performed after culture, revealing that 88.2% (30/34) of milk samples and 93.9% (31/33) of human samples were variably positive to those genes.
Conclusion: The use of nuc gene based PCR is an accurate and rapid method for S aureus isolates detection. A high prevalence of multiple drug-resistant isolates of S. aureus recovered from both human and milk represents further evidence for possible veterinary hazards as well as public hazards, especially to those that consume milk from this region. |
| first_indexed | 2024-04-12T22:14:02Z |
| format | Article |
| id | doaj.art-47b7bacadc4b4816833d04f97d36168c |
| institution | Directory Open Access Journal |
| issn | 2455-5673 2455-8931 |
| language | English |
| last_indexed | 2024-04-12T22:14:02Z |
| publishDate | 2021-03-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | International Journal of One Health |
| spelling | doaj.art-47b7bacadc4b4816833d04f97d36168c2022-12-22T03:14:37ZengVeterinary WorldInternational Journal of One Health2455-56732455-89312021-03-0171707710.14202/IJOH.2021.70-77Molecular detection of Staphylococcus aureus enterotoxin genes isolated from mastitic milk and humans in El-Behira, EgyptMohamed S. Diab0https://orcid.org/0000-0002-4878-1534Nermin A. Ibrahim1https://orcid.org/0000-0003-4859-3717Yasser F. Elnaker2https://orcid.org/0000-0003-1655-0501Sherif A. Zidan3https://orcid.org/0000-0001-5400-6131Marwa A. Saad4https://orcid.org/0000-0002-5739-8705Department of Animal Hygiene and Zoonoses, Faculty of Veterinary Medicine, New Valley University, Egypt.Department of Bacteriology, Faculty of Veterinary Medicine, Mansoura University, Egypt.Department of Animal Medicine (Infectious Diseases), Faculty of Veterinary Medicine, New Valley University, Egypt.Department of Animal Hygiene and Zoonoses, Faculty of Veterinary Medicine, University of Sadat City, Egypt.Department of Food Control, Faculty of Veterinary Medicine, ShebinAl-Kom, Menofia University, Menofia, Egypt.Background and Aim: Milk is a chief source of many nutrients. However, we must also bear in mind that it is a potential source for many cases of food poisoning. This study was conducted to investigate the prevalence of cow mastitis and evaluate the presence of enterotoxins and antibiotic resistance patterns in Staphylococcus aureus isolated from milk and contact humans in El-Behira Province, Egypt. Materials and Methods: A total of 680 milk samples from 170 cows and 86 human samples consisting of 43 hand swabs and 43 nasal swabs were analyzed. The milk samples were subjected to the California mastitis test. Results: The general occurrence was 23.1% (157/680) where 48 quarters had clinical mastitis and 109 had subclinical mastitis. Subsequently, S. aureus was isolated in Baird-Parker agar where typical and atypical colonies were selected and submitted to coagulase and complementary tests. Out of 48 samples of mastitic milk studied, 16 (33.3%) showed contamination by S. aureus whereas 109 samples of subclinical mastitis showed contamination in only 18 (16.5%). On the opposite hand, of the 86 human samples, 33 revealed S. aureus contamination, corresponding to 38.37% of the samples. Furthermore, multiplex polymerase chain reaction targeting nuc and the staphylococcal enterotoxin-encoding genes sea, seb, sec, sed, and see were performed after culture, revealing that 88.2% (30/34) of milk samples and 93.9% (31/33) of human samples were variably positive to those genes. Conclusion: The use of nuc gene based PCR is an accurate and rapid method for S aureus isolates detection. A high prevalence of multiple drug-resistant isolates of S. aureus recovered from both human and milk represents further evidence for possible veterinary hazards as well as public hazards, especially to those that consume milk from this region.https://www.onehealthjournal.org/Vol.7/No.1/9.pdfantibiotic sensitivitydairy productionenterotoxinsmultidrug resistancemultiplex polymerase chain reactionnuc genestaphylococcus aureus enterotoxin genesstaphylococcus aureus |
| spellingShingle | Mohamed S. Diab Nermin A. Ibrahim Yasser F. Elnaker Sherif A. Zidan Marwa A. Saad Molecular detection of Staphylococcus aureus enterotoxin genes isolated from mastitic milk and humans in El-Behira, Egypt International Journal of One Health antibiotic sensitivity dairy production enterotoxins multidrug resistance multiplex polymerase chain reaction nuc gene staphylococcus aureus enterotoxin genes staphylococcus aureus |
| title | Molecular detection of Staphylococcus aureus enterotoxin genes isolated from mastitic milk and humans in El-Behira, Egypt |
| title_full | Molecular detection of Staphylococcus aureus enterotoxin genes isolated from mastitic milk and humans in El-Behira, Egypt |
| title_fullStr | Molecular detection of Staphylococcus aureus enterotoxin genes isolated from mastitic milk and humans in El-Behira, Egypt |
| title_full_unstemmed | Molecular detection of Staphylococcus aureus enterotoxin genes isolated from mastitic milk and humans in El-Behira, Egypt |
| title_short | Molecular detection of Staphylococcus aureus enterotoxin genes isolated from mastitic milk and humans in El-Behira, Egypt |
| title_sort | molecular detection of staphylococcus aureus enterotoxin genes isolated from mastitic milk and humans in el behira egypt |
| topic | antibiotic sensitivity dairy production enterotoxins multidrug resistance multiplex polymerase chain reaction nuc gene staphylococcus aureus enterotoxin genes staphylococcus aureus |
| url | https://www.onehealthjournal.org/Vol.7/No.1/9.pdf |
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