Attachment of Respiratory Pathogens and <i>Candida</i> to Denture Base Materials—A Pilot Study
Denture prostheses are an ideal and extensive reservoir for microorganisms to attach to their surfaces. The aim of the study was to elucidate interactions between materials for the fabrication of denture bases and the attachment of microorganisms, focusing on respiratory pathogens and <i>Candi...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-09-01
|
| Series: | Journal of Clinical Medicine |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2077-0383/12/19/6127 |
| _version_ | 1797575675390984192 |
|---|---|
| author | Anne Schmutzler Catalina Suzana Stingu Elena Günther Reinhold Lang Florian Fuchs Andreas Koenig Angelika Rauch Sebastian Hahnel |
| author_facet | Anne Schmutzler Catalina Suzana Stingu Elena Günther Reinhold Lang Florian Fuchs Andreas Koenig Angelika Rauch Sebastian Hahnel |
| author_sort | Anne Schmutzler |
| collection | DOAJ |
| description | Denture prostheses are an ideal and extensive reservoir for microorganisms to attach to their surfaces. The aim of the study was to elucidate interactions between materials for the fabrication of denture bases and the attachment of microorganisms, focusing on respiratory pathogens and <i>Candida</i> species. Specimens (6 mm × 1 mm) with a standardized surface roughness (Sa = 0.1 µm) were prepared from heat-pressed polymethyl methacrylate (PMMA), CAD/CAM-processed PMMA, and CAD/CAM-processed polyether ether ketone (PEEK). The specimens were randomly placed in the vestibular areas of complete upper dentures in seven patients and were removed either after 24 h without any oral hygiene measures or after a period of four weeks. The microorganisms adherent to the surface of the specimens were cultivated and subsequently analyzed using mass spectrometry (MALDI-TOF). The means and standard deviations were calculated, and the data were analyzed using a two-way analysis of variance (ANOVA) and Tukey post-hoc test where appropriate (α = 0.05). There was a significant increase (<i>p</i> ≤ 0.004) in the total bacterial counts (CFU/mL) between the first (24 h) and the second (four weeks) measurements. Regarding quantitative microbiological analyses, no significant differences between the various materials were identified. Respiratory microorganisms were detected in all samples at both measurement time points, with a large variance between different patients. Only after four weeks, <i>Candida</i> species were identified on all materials but not in all participants. <i>Candida</i> species and respiratory microorganisms accumulate on various denture base resins. While no significant differences were identified between the materials, there was a tendency towards a more pronounced accumulation of microorganisms on conventionally processed PMMA. |
| first_indexed | 2024-03-10T21:42:37Z |
| format | Article |
| id | doaj.art-47b7ef5529204bfb914f6fd3f0246177 |
| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-10T21:42:37Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-47b7ef5529204bfb914f6fd3f02461772023-11-19T14:34:29ZengMDPI AGJournal of Clinical Medicine2077-03832023-09-011219612710.3390/jcm12196127Attachment of Respiratory Pathogens and <i>Candida</i> to Denture Base Materials—A Pilot StudyAnne Schmutzler0Catalina Suzana Stingu1Elena Günther2Reinhold Lang3Florian Fuchs4Andreas Koenig5Angelika Rauch6Sebastian Hahnel7Department of Prosthetic Dentistry, Regensburg University Medical Center, 93042 Regensburg, GermanyInstitute for Medical Microbiology and Virology, Leipzig University Clinics, 04103 Leipzig, GermanyDepartment of Prosthetic Dentistry and Dental Materials Science, Leipzig University, 04103 Leipzig, GermanyDepartment of Prosthetic Dentistry, Regensburg University Medical Center, 93042 Regensburg, GermanyDepartment of Prosthetic Dentistry and Dental Materials Science, Leipzig University, 04103 Leipzig, GermanyDepartment of Prosthetic Dentistry and Dental Materials Science, Leipzig University, 04103 Leipzig, GermanyDepartment of Prosthetic Dentistry, Regensburg University Medical Center, 93042 Regensburg, GermanyDepartment of Prosthetic Dentistry, Regensburg University Medical Center, 93042 Regensburg, GermanyDenture prostheses are an ideal and extensive reservoir for microorganisms to attach to their surfaces. The aim of the study was to elucidate interactions between materials for the fabrication of denture bases and the attachment of microorganisms, focusing on respiratory pathogens and <i>Candida</i> species. Specimens (6 mm × 1 mm) with a standardized surface roughness (Sa = 0.1 µm) were prepared from heat-pressed polymethyl methacrylate (PMMA), CAD/CAM-processed PMMA, and CAD/CAM-processed polyether ether ketone (PEEK). The specimens were randomly placed in the vestibular areas of complete upper dentures in seven patients and were removed either after 24 h without any oral hygiene measures or after a period of four weeks. The microorganisms adherent to the surface of the specimens were cultivated and subsequently analyzed using mass spectrometry (MALDI-TOF). The means and standard deviations were calculated, and the data were analyzed using a two-way analysis of variance (ANOVA) and Tukey post-hoc test where appropriate (α = 0.05). There was a significant increase (<i>p</i> ≤ 0.004) in the total bacterial counts (CFU/mL) between the first (24 h) and the second (four weeks) measurements. Regarding quantitative microbiological analyses, no significant differences between the various materials were identified. Respiratory microorganisms were detected in all samples at both measurement time points, with a large variance between different patients. Only after four weeks, <i>Candida</i> species were identified on all materials but not in all participants. <i>Candida</i> species and respiratory microorganisms accumulate on various denture base resins. While no significant differences were identified between the materials, there was a tendency towards a more pronounced accumulation of microorganisms on conventionally processed PMMA.https://www.mdpi.com/2077-0383/12/19/6127biofilmPMMAPEEK<i>Candida</i>dentureprosthesis |
| spellingShingle | Anne Schmutzler Catalina Suzana Stingu Elena Günther Reinhold Lang Florian Fuchs Andreas Koenig Angelika Rauch Sebastian Hahnel Attachment of Respiratory Pathogens and <i>Candida</i> to Denture Base Materials—A Pilot Study Journal of Clinical Medicine biofilm PMMA PEEK <i>Candida</i> denture prosthesis |
| title | Attachment of Respiratory Pathogens and <i>Candida</i> to Denture Base Materials—A Pilot Study |
| title_full | Attachment of Respiratory Pathogens and <i>Candida</i> to Denture Base Materials—A Pilot Study |
| title_fullStr | Attachment of Respiratory Pathogens and <i>Candida</i> to Denture Base Materials—A Pilot Study |
| title_full_unstemmed | Attachment of Respiratory Pathogens and <i>Candida</i> to Denture Base Materials—A Pilot Study |
| title_short | Attachment of Respiratory Pathogens and <i>Candida</i> to Denture Base Materials—A Pilot Study |
| title_sort | attachment of respiratory pathogens and i candida i to denture base materials a pilot study |
| topic | biofilm PMMA PEEK <i>Candida</i> denture prosthesis |
| url | https://www.mdpi.com/2077-0383/12/19/6127 |
| work_keys_str_mv | AT anneschmutzler attachmentofrespiratorypathogensandicandidaitodenturebasematerialsapilotstudy AT catalinasuzanastingu attachmentofrespiratorypathogensandicandidaitodenturebasematerialsapilotstudy AT elenagunther attachmentofrespiratorypathogensandicandidaitodenturebasematerialsapilotstudy AT reinholdlang attachmentofrespiratorypathogensandicandidaitodenturebasematerialsapilotstudy AT florianfuchs attachmentofrespiratorypathogensandicandidaitodenturebasematerialsapilotstudy AT andreaskoenig attachmentofrespiratorypathogensandicandidaitodenturebasematerialsapilotstudy AT angelikarauch attachmentofrespiratorypathogensandicandidaitodenturebasematerialsapilotstudy AT sebastianhahnel attachmentofrespiratorypathogensandicandidaitodenturebasematerialsapilotstudy |