Data Mining by Pluralistic Approach on CRISPR Gene Editing in Plants
Genome engineering by site-specific nucleases enables reverse genetics and targeted editing of genomes in an efficacious manner. Contemporary revolutionized progress in targeted-genome engineering technologies based on Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-related RNA-gu...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-07-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2019.00801/full |
| _version_ | 1818612788919009280 |
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| author | Tanushri Kaul Nitya Meenakshi Raman Murugesh Eswaran Arulprakash Thangaraj Rachana Verma Sonia Khan Sony Krishnamurthy M. Sathelly Rashmi Kaul Pranjal Yadava Pawan Kumar Agrawal |
| author_facet | Tanushri Kaul Nitya Meenakshi Raman Murugesh Eswaran Arulprakash Thangaraj Rachana Verma Sonia Khan Sony Krishnamurthy M. Sathelly Rashmi Kaul Pranjal Yadava Pawan Kumar Agrawal |
| author_sort | Tanushri Kaul |
| collection | DOAJ |
| description | Genome engineering by site-specific nucleases enables reverse genetics and targeted editing of genomes in an efficacious manner. Contemporary revolutionized progress in targeted-genome engineering technologies based on Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-related RNA-guided endonucleases facilitate coherent interrogation of crop genome function. Evolved as an innate component of the adaptive immune response in bacterial and archaeal systems, CRISPR/Cas system is now identified as a versatile molecular tool that ensures specific and targeted genome modification in plants. Applications of this genome redaction tool-kit include somatic genome editing, rectification of genetic disorders or gene therapy, treatment of infectious diseases, generation of animal models, and crop improvement. We review the utilization of these synthetic nucleases as precision, targeted-genome editing platforms with the inherent potential to accentuate basic science “strengths and shortcomings” of gene function, complement plant breeding techniques for crop improvement, and charter a knowledge base for effective use of editing technology for ever-increasing agricultural demands. Furthermore, the emerging importance of Cpf1, Cas9 nickase, C2c2, as well as other innovative candidates that may prove more effective in driving novel applications in crops are also discussed. The mined data has been prepared as a library and opened for public use at www.lipre.org. |
| first_indexed | 2024-12-16T15:51:48Z |
| format | Article |
| id | doaj.art-47bab04f61ab4c56a3c0f72d17296843 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-16T15:51:48Z |
| publishDate | 2019-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-47bab04f61ab4c56a3c0f72d172968432022-12-21T22:25:41ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2019-07-011010.3389/fpls.2019.00801436246Data Mining by Pluralistic Approach on CRISPR Gene Editing in PlantsTanushri Kaul0Nitya Meenakshi Raman1Murugesh Eswaran2Arulprakash Thangaraj3Rachana Verma4Sonia Khan Sony5Krishnamurthy M. Sathelly6Rashmi Kaul7Pranjal Yadava8Pawan Kumar Agrawal9Nutritional Improvement of Crops Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, IndiaNutritional Improvement of Crops Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, IndiaNutritional Improvement of Crops Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, IndiaNutritional Improvement of Crops Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, IndiaNutritional Improvement of Crops Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, IndiaNutritional Improvement of Crops Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, IndiaNutritional Improvement of Crops Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, IndiaNutritional Improvement of Crops Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, IndiaDepartment of Biotechnology, Indian Institute of Maize Research, Indian Institute of Agricultural Biotechnology (ICAR), New Delhi, IndiaNational Agricultural Science Fund, Indian Council of Agricultural Research, New Delhi, IndiaGenome engineering by site-specific nucleases enables reverse genetics and targeted editing of genomes in an efficacious manner. Contemporary revolutionized progress in targeted-genome engineering technologies based on Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-related RNA-guided endonucleases facilitate coherent interrogation of crop genome function. Evolved as an innate component of the adaptive immune response in bacterial and archaeal systems, CRISPR/Cas system is now identified as a versatile molecular tool that ensures specific and targeted genome modification in plants. Applications of this genome redaction tool-kit include somatic genome editing, rectification of genetic disorders or gene therapy, treatment of infectious diseases, generation of animal models, and crop improvement. We review the utilization of these synthetic nucleases as precision, targeted-genome editing platforms with the inherent potential to accentuate basic science “strengths and shortcomings” of gene function, complement plant breeding techniques for crop improvement, and charter a knowledge base for effective use of editing technology for ever-increasing agricultural demands. Furthermore, the emerging importance of Cpf1, Cas9 nickase, C2c2, as well as other innovative candidates that may prove more effective in driving novel applications in crops are also discussed. The mined data has been prepared as a library and opened for public use at www.lipre.org.https://www.frontiersin.org/article/10.3389/fpls.2019.00801/fullgenomesgRNAdouble-stranded breaknon-homologous end joining repairhomology-directed repairCas9 |
| spellingShingle | Tanushri Kaul Nitya Meenakshi Raman Murugesh Eswaran Arulprakash Thangaraj Rachana Verma Sonia Khan Sony Krishnamurthy M. Sathelly Rashmi Kaul Pranjal Yadava Pawan Kumar Agrawal Data Mining by Pluralistic Approach on CRISPR Gene Editing in Plants Frontiers in Plant Science genome sgRNA double-stranded break non-homologous end joining repair homology-directed repair Cas9 |
| title | Data Mining by Pluralistic Approach on CRISPR Gene Editing in Plants |
| title_full | Data Mining by Pluralistic Approach on CRISPR Gene Editing in Plants |
| title_fullStr | Data Mining by Pluralistic Approach on CRISPR Gene Editing in Plants |
| title_full_unstemmed | Data Mining by Pluralistic Approach on CRISPR Gene Editing in Plants |
| title_short | Data Mining by Pluralistic Approach on CRISPR Gene Editing in Plants |
| title_sort | data mining by pluralistic approach on crispr gene editing in plants |
| topic | genome sgRNA double-stranded break non-homologous end joining repair homology-directed repair Cas9 |
| url | https://www.frontiersin.org/article/10.3389/fpls.2019.00801/full |
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