Antibacterial Activity of Silver Nanoparticles against Staphylococcus warneri Synthesized Using Endophytic Bacteria by Photo-irradiation
Diseases caused by Staphylococcus warneri have a significant impact on human health. We evaluated the antibacterial activity of silver nanoparticles (synthesized using the endophytic strain SYSU 333150) against S. warneri. The strain SYSU 333150 was isolated from the roots of Borszczowia aralocaspic...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-06-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2017.01090/full |
| _version_ | 1828520726919905280 |
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| author | Zhou-Yan Dong Manik Prabhu Narsing Rao Min Xiao Hong-Fei Wang Hong-Fei Wang Wael N. Hozzein Wael N. Hozzein Wei Chen Wen-Jun Li Wen-Jun Li |
| author_facet | Zhou-Yan Dong Manik Prabhu Narsing Rao Min Xiao Hong-Fei Wang Hong-Fei Wang Wael N. Hozzein Wael N. Hozzein Wei Chen Wen-Jun Li Wen-Jun Li |
| author_sort | Zhou-Yan Dong |
| collection | DOAJ |
| description | Diseases caused by Staphylococcus warneri have a significant impact on human health. We evaluated the antibacterial activity of silver nanoparticles (synthesized using the endophytic strain SYSU 333150) against S. warneri. The strain SYSU 333150 was isolated from the roots of Borszczowia aralocaspica Bunge. The 16S rRNA sequence results suggest that SYSU 333150 belongs to the genus Isoptericola and is likely a new species. Photo-irradiation was used to synthesize silver nanoparticles, which were characterized using UV-visible spectroscopy, transmission electron microscopy and X-ray diffraction. The nanoparticles were spherical and measured to be11 to 40 nm. X-ray diffraction revealed four peaks corresponding to the 111, 200, 220, and 311 planes of the face-centered cubic lattice, indicating a crystalline nature. Fourier transform infrared spectroscopy suggested that the metabolites in the culture supernatant were likely reducing and capping agents. The silver nanoparticles possessed antimicrobial activity (14 mm zone of inhibition) against S. warneri, which was likely a result of DNA cleavage. The synthesized silver nanoparticles have potent antibacterial activity against S. warneri and can be used to control infection. |
| first_indexed | 2024-12-11T19:35:53Z |
| format | Article |
| id | doaj.art-47bae5374a5142029f2779c7f8f22377 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-11T19:35:53Z |
| publishDate | 2017-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-47bae5374a5142029f2779c7f8f223772022-12-22T00:53:09ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-06-01810.3389/fmicb.2017.01090240691Antibacterial Activity of Silver Nanoparticles against Staphylococcus warneri Synthesized Using Endophytic Bacteria by Photo-irradiationZhou-Yan Dong0Manik Prabhu Narsing Rao1Min Xiao2Hong-Fei Wang3Hong-Fei Wang4Wael N. Hozzein5Wael N. Hozzein6Wei Chen7Wen-Jun Li8Wen-Jun Li9State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen UniversityGuangzhou, ChinaState Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen UniversityGuangzhou, ChinaState Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen UniversityGuangzhou, ChinaCollege of Life Science, Liaoning Normal UniversityDalian, ChinaChina Tobacco Yunnan Industrial Co., Ltd.Kunming, ChinaBioproducts Research Chair, Zoology Department, College of Science, King Saud UniversityRiyadh, Saudi ArabiaBotany and Microbiology Department, Faculty of Science, Beni-Suef UniversityBeni-Suef, EgyptChina Tobacco Yunnan Industrial Co., Ltd.Kunming, ChinaState Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen UniversityGuangzhou, ChinaKey Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of SciencesÜrűmqi, ChinaDiseases caused by Staphylococcus warneri have a significant impact on human health. We evaluated the antibacterial activity of silver nanoparticles (synthesized using the endophytic strain SYSU 333150) against S. warneri. The strain SYSU 333150 was isolated from the roots of Borszczowia aralocaspica Bunge. The 16S rRNA sequence results suggest that SYSU 333150 belongs to the genus Isoptericola and is likely a new species. Photo-irradiation was used to synthesize silver nanoparticles, which were characterized using UV-visible spectroscopy, transmission electron microscopy and X-ray diffraction. The nanoparticles were spherical and measured to be11 to 40 nm. X-ray diffraction revealed four peaks corresponding to the 111, 200, 220, and 311 planes of the face-centered cubic lattice, indicating a crystalline nature. Fourier transform infrared spectroscopy suggested that the metabolites in the culture supernatant were likely reducing and capping agents. The silver nanoparticles possessed antimicrobial activity (14 mm zone of inhibition) against S. warneri, which was likely a result of DNA cleavage. The synthesized silver nanoparticles have potent antibacterial activity against S. warneri and can be used to control infection.http://journal.frontiersin.org/article/10.3389/fmicb.2017.01090/fullsilver nanoparticlesendophyteStaphylococcus warneriantibacterial activityDNA cleavage study |
| spellingShingle | Zhou-Yan Dong Manik Prabhu Narsing Rao Min Xiao Hong-Fei Wang Hong-Fei Wang Wael N. Hozzein Wael N. Hozzein Wei Chen Wen-Jun Li Wen-Jun Li Antibacterial Activity of Silver Nanoparticles against Staphylococcus warneri Synthesized Using Endophytic Bacteria by Photo-irradiation Frontiers in Microbiology silver nanoparticles endophyte Staphylococcus warneri antibacterial activity DNA cleavage study |
| title | Antibacterial Activity of Silver Nanoparticles against Staphylococcus warneri Synthesized Using Endophytic Bacteria by Photo-irradiation |
| title_full | Antibacterial Activity of Silver Nanoparticles against Staphylococcus warneri Synthesized Using Endophytic Bacteria by Photo-irradiation |
| title_fullStr | Antibacterial Activity of Silver Nanoparticles against Staphylococcus warneri Synthesized Using Endophytic Bacteria by Photo-irradiation |
| title_full_unstemmed | Antibacterial Activity of Silver Nanoparticles against Staphylococcus warneri Synthesized Using Endophytic Bacteria by Photo-irradiation |
| title_short | Antibacterial Activity of Silver Nanoparticles against Staphylococcus warneri Synthesized Using Endophytic Bacteria by Photo-irradiation |
| title_sort | antibacterial activity of silver nanoparticles against staphylococcus warneri synthesized using endophytic bacteria by photo irradiation |
| topic | silver nanoparticles endophyte Staphylococcus warneri antibacterial activity DNA cleavage study |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2017.01090/full |
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