Efficient gene delivery and selective transduction of astrocytes in the mammalian brain using viral vectors
Astrocytes are now considered as key players in brain information processing because of their newly discovered roles in synapse formation and plasticity, energy metabolism and blood flow regulation. However, our understanding of astrocyte function is still fragmented compared to other brain cell typ...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2013-07-01
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| Series: | Frontiers in Cellular Neuroscience |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fncel.2013.00106/full |
| _version_ | 1811238775592845312 |
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| author | Nicolas eMerienne Juliette eLe Douce Emilie eFaivre Nicole eDéglon Gilles eBonvento |
| author_facet | Nicolas eMerienne Juliette eLe Douce Emilie eFaivre Nicole eDéglon Gilles eBonvento |
| author_sort | Nicolas eMerienne |
| collection | DOAJ |
| description | Astrocytes are now considered as key players in brain information processing because of their newly discovered roles in synapse formation and plasticity, energy metabolism and blood flow regulation. However, our understanding of astrocyte function is still fragmented compared to other brain cell types. A better appreciation of the biology of astrocytes requires the development of tools to generate animal models in which astrocyte-specific proteins and pathways can be manipulated. In addition, it is becoming increasingly evident that astrocytes are also important players in many neurological disorders. Targeted modulation of protein expression in astrocytes would be critical for the development of new therapeutic strategies. Gene transfer is valuable to target a subpopulation of cells and explore their function in experimental models. In particular, viral-mediated gene transfer provides a rapid, highly flexible and cost-effective, in vivo paradigm to study the impact of genes of interest during CNS development or in adult animals. We will review the different strategies that led to the recent development of efficient viral vectors that can be successfully used to selectively transduce astrocytes in the mammalian brain. |
| first_indexed | 2024-04-12T12:48:10Z |
| format | Article |
| id | doaj.art-47be634683304bf7ad3f31cdb7d1fc3c |
| institution | Directory Open Access Journal |
| issn | 1662-5102 |
| language | English |
| last_indexed | 2024-04-12T12:48:10Z |
| publishDate | 2013-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular Neuroscience |
| spelling | doaj.art-47be634683304bf7ad3f31cdb7d1fc3c2022-12-22T03:32:33ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022013-07-01710.3389/fncel.2013.0010654254Efficient gene delivery and selective transduction of astrocytes in the mammalian brain using viral vectorsNicolas eMerienne0Juliette eLe Douce1Emilie eFaivre2Nicole eDéglon3Gilles eBonvento4Lausanne University Hospital (CHUV)CEACEALausanne University Hospital (CHUV)CEAAstrocytes are now considered as key players in brain information processing because of their newly discovered roles in synapse formation and plasticity, energy metabolism and blood flow regulation. However, our understanding of astrocyte function is still fragmented compared to other brain cell types. A better appreciation of the biology of astrocytes requires the development of tools to generate animal models in which astrocyte-specific proteins and pathways can be manipulated. In addition, it is becoming increasingly evident that astrocytes are also important players in many neurological disorders. Targeted modulation of protein expression in astrocytes would be critical for the development of new therapeutic strategies. Gene transfer is valuable to target a subpopulation of cells and explore their function in experimental models. In particular, viral-mediated gene transfer provides a rapid, highly flexible and cost-effective, in vivo paradigm to study the impact of genes of interest during CNS development or in adult animals. We will review the different strategies that led to the recent development of efficient viral vectors that can be successfully used to selectively transduce astrocytes in the mammalian brain.http://journal.frontiersin.org/Journal/10.3389/fncel.2013.00106/fullAstrocytesGene TherapyTropismLentiviral vectorsAAV vectors |
| spellingShingle | Nicolas eMerienne Juliette eLe Douce Emilie eFaivre Nicole eDéglon Gilles eBonvento Efficient gene delivery and selective transduction of astrocytes in the mammalian brain using viral vectors Frontiers in Cellular Neuroscience Astrocytes Gene Therapy Tropism Lentiviral vectors AAV vectors |
| title | Efficient gene delivery and selective transduction of astrocytes in the mammalian brain using viral vectors |
| title_full | Efficient gene delivery and selective transduction of astrocytes in the mammalian brain using viral vectors |
| title_fullStr | Efficient gene delivery and selective transduction of astrocytes in the mammalian brain using viral vectors |
| title_full_unstemmed | Efficient gene delivery and selective transduction of astrocytes in the mammalian brain using viral vectors |
| title_short | Efficient gene delivery and selective transduction of astrocytes in the mammalian brain using viral vectors |
| title_sort | efficient gene delivery and selective transduction of astrocytes in the mammalian brain using viral vectors |
| topic | Astrocytes Gene Therapy Tropism Lentiviral vectors AAV vectors |
| url | http://journal.frontiersin.org/Journal/10.3389/fncel.2013.00106/full |
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