Identification and Genetic Characterization of Fasciola hepatica Isolated from Cattle in Jeddah, Saudi Arabia Based on Sequence Analysis of Mitochondrial (COI) Gene
Muslimah N Alsulami,1 Majed H Wakid,2 Mohammed Al-matary,3 Rewaida Abdel-Gaber,4 Wafa Abdullah I Al-Megrin,5 Areej O Bakhraibah,1 Abdullah D Alanazi,6 Hatem A Elshabrawy,7 Asmaa M El-Kady8 1Department of Biology, College of Science, University of Jeddah, Jeddah, 21493, Saudi Arabia; 2Department of M...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2022-08-01
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| Series: | Infection and Drug Resistance |
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| Online Access: | https://www.dovepress.com/identification-and-genetic-characterization-of-fasciola-hepatica-isola-peer-reviewed-fulltext-article-IDR |
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| author | Alsulami MN Wakid MH Al-matary M Abdel-Gaber R Al-Megrin WAI Bakhraibah AO Alanazi AD Elshabrawy HA El-Kady AM |
| author_facet | Alsulami MN Wakid MH Al-matary M Abdel-Gaber R Al-Megrin WAI Bakhraibah AO Alanazi AD Elshabrawy HA El-Kady AM |
| author_sort | Alsulami MN |
| collection | DOAJ |
| description | Muslimah N Alsulami,1 Majed H Wakid,2 Mohammed Al-matary,3 Rewaida Abdel-Gaber,4 Wafa Abdullah I Al-Megrin,5 Areej O Bakhraibah,1 Abdullah D Alanazi,6 Hatem A Elshabrawy,7 Asmaa M El-Kady8 1Department of Biology, College of Science, University of Jeddah, Jeddah, 21493, Saudi Arabia; 2Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, 21589, Saudi Arabia; 3Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, 21589, Saudi Arabia; 4Department of Zoology, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia; 5Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh, 11671, Saudi Arabia; 6Department of Biological Sciences, Faculty of Science and Humanities, Shaqra University, Ad-Dawadimi, 11911, Saudi Arabia; 7Department of Molecular and Cellular Biology, College of Osteopathic Medicine, Sam Houston State University, Conroe, TX, 77304, USA; 8Department of Medical Parasitology, Faculty of Medicine, South Valley University, Qena, 83523, EgyptCorrespondence: Asmaa M El-Kady; Hatem A Elshabrawy, Email Asmaa.elkady@med.svu.edu.eg; hatem.elshabrawy@shsu.eduBackground: In Saudi Arabia, more than US$ 0.2 million annual losses are caused by liver condemnations due to fascioliasis. Data obtained from the genetic characterization of Fasciola population sheds light on parasite transmission which could eventually help in development of effective parasite control measures. So, the aim of this study was to investigate the genetic diversity of Fasciola spp. isolated from cattle in Saudi Arabia by sequence analyses of COI gene.Materials and Methods: A total of 325 cows slaughtered at the central municipal abattoir in Jeddah city, Jeddah Province, Saudi Arabia were examined for fascioliasis in the period from 1st of June to 1st of July 2020. DNA was extracted from adult Fasciola worms and used for PCR and DNA sequence using a primer pair targeting COI gene. Analysis of the obtained sequences was done using BLAST search and phylogenetic analysis.Results: Bovine fascioliasis was diagnosed in 18 out of 325 cattle (5.5%). Forty-eight flukes were extracted from infected animals and DNA was successfully amplified from all flukes. Overall 12 different DNA sequences were obtained. BLAST search showed that all obtained sequences were F. hepatica and had > 97% similarity with F. hepatica isolates from Tanzania, Europe and Iran. Phylogenetic analysis of the obtained sequences showed that Fasciola isolates from the current study were clustered in one subclade closely related to isolates from North and South Africa and Italy.Conclusion: Reports on the molecular characterization of Fasciola spp. in Saudi Arabia are limited. In the current study, our findings showed that F. hepatica was the only Fasciola species parasitizing cattle in Jeddah city, Saudi Arabia. Further studies using a large number of samples from different localities in Saudi Arabia are needed to provide data that will help the development of control measures against fascioliasis.Keywords: fascioliasis, cattle, PCR, COI, Saudi Arabia |
| first_indexed | 2024-04-12T23:44:42Z |
| format | Article |
| id | doaj.art-47bf006e9d3b46e49f7d0fe90de70851 |
| institution | Directory Open Access Journal |
| issn | 1178-6973 |
| language | English |
| last_indexed | 2024-04-12T23:44:42Z |
| publishDate | 2022-08-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Infection and Drug Resistance |
| spelling | doaj.art-47bf006e9d3b46e49f7d0fe90de708512022-12-22T03:11:53ZengDove Medical PressInfection and Drug Resistance1178-69732022-08-01Volume 154877488677698Identification and Genetic Characterization of Fasciola hepatica Isolated from Cattle in Jeddah, Saudi Arabia Based on Sequence Analysis of Mitochondrial (COI) GeneAlsulami MNWakid MHAl-matary MAbdel-Gaber RAl-Megrin WAIBakhraibah AOAlanazi ADElshabrawy HAEl-Kady AMMuslimah N Alsulami,1 Majed H Wakid,2 Mohammed Al-matary,3 Rewaida Abdel-Gaber,4 Wafa Abdullah I Al-Megrin,5 Areej O Bakhraibah,1 Abdullah D Alanazi,6 Hatem A Elshabrawy,7 Asmaa M El-Kady8 1Department of Biology, College of Science, University of Jeddah, Jeddah, 21493, Saudi Arabia; 2Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, 21589, Saudi Arabia; 3Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, 21589, Saudi Arabia; 4Department of Zoology, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia; 5Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh, 11671, Saudi Arabia; 6Department of Biological Sciences, Faculty of Science and Humanities, Shaqra University, Ad-Dawadimi, 11911, Saudi Arabia; 7Department of Molecular and Cellular Biology, College of Osteopathic Medicine, Sam Houston State University, Conroe, TX, 77304, USA; 8Department of Medical Parasitology, Faculty of Medicine, South Valley University, Qena, 83523, EgyptCorrespondence: Asmaa M El-Kady; Hatem A Elshabrawy, Email Asmaa.elkady@med.svu.edu.eg; hatem.elshabrawy@shsu.eduBackground: In Saudi Arabia, more than US$ 0.2 million annual losses are caused by liver condemnations due to fascioliasis. Data obtained from the genetic characterization of Fasciola population sheds light on parasite transmission which could eventually help in development of effective parasite control measures. So, the aim of this study was to investigate the genetic diversity of Fasciola spp. isolated from cattle in Saudi Arabia by sequence analyses of COI gene.Materials and Methods: A total of 325 cows slaughtered at the central municipal abattoir in Jeddah city, Jeddah Province, Saudi Arabia were examined for fascioliasis in the period from 1st of June to 1st of July 2020. DNA was extracted from adult Fasciola worms and used for PCR and DNA sequence using a primer pair targeting COI gene. Analysis of the obtained sequences was done using BLAST search and phylogenetic analysis.Results: Bovine fascioliasis was diagnosed in 18 out of 325 cattle (5.5%). Forty-eight flukes were extracted from infected animals and DNA was successfully amplified from all flukes. Overall 12 different DNA sequences were obtained. BLAST search showed that all obtained sequences were F. hepatica and had > 97% similarity with F. hepatica isolates from Tanzania, Europe and Iran. Phylogenetic analysis of the obtained sequences showed that Fasciola isolates from the current study were clustered in one subclade closely related to isolates from North and South Africa and Italy.Conclusion: Reports on the molecular characterization of Fasciola spp. in Saudi Arabia are limited. In the current study, our findings showed that F. hepatica was the only Fasciola species parasitizing cattle in Jeddah city, Saudi Arabia. Further studies using a large number of samples from different localities in Saudi Arabia are needed to provide data that will help the development of control measures against fascioliasis.Keywords: fascioliasis, cattle, PCR, COI, Saudi Arabiahttps://www.dovepress.com/identification-and-genetic-characterization-of-fasciola-hepatica-isola-peer-reviewed-fulltext-article-IDRfascioliasiscattlepcrcoisaudi arabia |
| spellingShingle | Alsulami MN Wakid MH Al-matary M Abdel-Gaber R Al-Megrin WAI Bakhraibah AO Alanazi AD Elshabrawy HA El-Kady AM Identification and Genetic Characterization of Fasciola hepatica Isolated from Cattle in Jeddah, Saudi Arabia Based on Sequence Analysis of Mitochondrial (COI) Gene Infection and Drug Resistance fascioliasis cattle pcr coi saudi arabia |
| title | Identification and Genetic Characterization of Fasciola hepatica Isolated from Cattle in Jeddah, Saudi Arabia Based on Sequence Analysis of Mitochondrial (COI) Gene |
| title_full | Identification and Genetic Characterization of Fasciola hepatica Isolated from Cattle in Jeddah, Saudi Arabia Based on Sequence Analysis of Mitochondrial (COI) Gene |
| title_fullStr | Identification and Genetic Characterization of Fasciola hepatica Isolated from Cattle in Jeddah, Saudi Arabia Based on Sequence Analysis of Mitochondrial (COI) Gene |
| title_full_unstemmed | Identification and Genetic Characterization of Fasciola hepatica Isolated from Cattle in Jeddah, Saudi Arabia Based on Sequence Analysis of Mitochondrial (COI) Gene |
| title_short | Identification and Genetic Characterization of Fasciola hepatica Isolated from Cattle in Jeddah, Saudi Arabia Based on Sequence Analysis of Mitochondrial (COI) Gene |
| title_sort | identification and genetic characterization of fasciola hepatica isolated from cattle in jeddah saudi arabia based on sequence analysis of mitochondrial coi gene |
| topic | fascioliasis cattle pcr coi saudi arabia |
| url | https://www.dovepress.com/identification-and-genetic-characterization-of-fasciola-hepatica-isola-peer-reviewed-fulltext-article-IDR |
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