Comparable survival in rats with intracranial glioblastoma irradiated with single-fraction conventional radiotherapy or FLASH radiotherapy
BackgroundRadiotherapy increases survival in patients with glioblastoma. However, the prescribed dose is limited by unwanted side effects on normal tissue. Previous experimental studies have shown that FLASH radiotherapy (FLASH-RT) can reduce these side effects. Still, it is important to establish a...
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| Format: | Article |
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Frontiers Media S.A.
2024-01-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2023.1309174/full |
| _version_ | 1797348350402494464 |
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| author | Emma Liljedahl Elise Konradsson Karin Linderfalk Emma Gustafsson Kristoffer Petersson Kristoffer Petersson Crister Ceberg Henrietta Nittby Redebrandt Henrietta Nittby Redebrandt |
| author_facet | Emma Liljedahl Elise Konradsson Karin Linderfalk Emma Gustafsson Kristoffer Petersson Kristoffer Petersson Crister Ceberg Henrietta Nittby Redebrandt Henrietta Nittby Redebrandt |
| author_sort | Emma Liljedahl |
| collection | DOAJ |
| description | BackgroundRadiotherapy increases survival in patients with glioblastoma. However, the prescribed dose is limited by unwanted side effects on normal tissue. Previous experimental studies have shown that FLASH radiotherapy (FLASH-RT) can reduce these side effects. Still, it is important to establish an equal anti-tumor efficacy comparing FLASH-RT to conventional radiotherapy (CONV-RT).MethodsFully immunocompetent Fischer 344 rats with the GFP-positive NS1 intracranial glioblastoma model were irradiated with CONV-RT or FLASH-RT in one fraction of 20 Gy, 25 Gy or 30 Gy. Animals were monitored for survival and acute dermal side effects. The brains were harvested upon euthanasia and tumors were examined post mortem.ResultsSurvival was significantly increased in animals irradiated with CONV-RT and FLASH-RT at 20 Gy and 25 Gy compared to control animals. The longest survival was reached in animals irradiated with FLASH-RT and CONV-RT at 25 Gy. Irradiation at 30 Gy did not lead to increased survival, despite smaller tumors. Tumor size correlated inversely with irradiation dose, both in animals treated with CONV-RT and FLASH-RT. Acute dermal side effects were mild, but only a small proportion of the animals were alive for evaluation of those side effects.ConclusionThe dose response was similar for CONV-RT and FLASH-RT in the present model. Tumor size upon the time of euthanasia correlated inversely with the irradiation dose. |
| first_indexed | 2024-03-08T12:04:36Z |
| format | Article |
| id | doaj.art-47c7575fea414a93bf023e157e2e6f72 |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-03-08T12:04:36Z |
| publishDate | 2024-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-47c7575fea414a93bf023e157e2e6f722024-01-23T10:46:17ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2024-01-011310.3389/fonc.2023.13091741309174Comparable survival in rats with intracranial glioblastoma irradiated with single-fraction conventional radiotherapy or FLASH radiotherapyEmma Liljedahl0Elise Konradsson1Karin Linderfalk2Emma Gustafsson3Kristoffer Petersson4Kristoffer Petersson5Crister Ceberg6Henrietta Nittby Redebrandt7Henrietta Nittby Redebrandt8The Rausing Laboratory, Division of Neurosurgery, Department of Clinical Sciences, Lund University, Lund, SwedenMedical Radiation Physics, Department of Clinical Sciences, Lund University, Lund, SwedenThe Rausing Laboratory, Division of Neurosurgery, Department of Clinical Sciences, Lund University, Lund, SwedenThe Rausing Laboratory, Division of Neurosurgery, Department of Clinical Sciences, Lund University, Lund, SwedenDepartment of Oncology, Medical Research Council Oxford Institute for Radiation Oncology, University of Oxford, Oxford, United KingdomRadiation Physics, Department of Haematology, Oncology and Radiation Physics, Skåne University Hospital, Lund University, Lund, SwedenMedical Radiation Physics, Department of Clinical Sciences, Lund University, Lund, SwedenThe Rausing Laboratory, Division of Neurosurgery, Department of Clinical Sciences, Lund University, Lund, SwedenDepartment of Neurosurgery, Skåne University Hospital, Lund, SwedenBackgroundRadiotherapy increases survival in patients with glioblastoma. However, the prescribed dose is limited by unwanted side effects on normal tissue. Previous experimental studies have shown that FLASH radiotherapy (FLASH-RT) can reduce these side effects. Still, it is important to establish an equal anti-tumor efficacy comparing FLASH-RT to conventional radiotherapy (CONV-RT).MethodsFully immunocompetent Fischer 344 rats with the GFP-positive NS1 intracranial glioblastoma model were irradiated with CONV-RT or FLASH-RT in one fraction of 20 Gy, 25 Gy or 30 Gy. Animals were monitored for survival and acute dermal side effects. The brains were harvested upon euthanasia and tumors were examined post mortem.ResultsSurvival was significantly increased in animals irradiated with CONV-RT and FLASH-RT at 20 Gy and 25 Gy compared to control animals. The longest survival was reached in animals irradiated with FLASH-RT and CONV-RT at 25 Gy. Irradiation at 30 Gy did not lead to increased survival, despite smaller tumors. Tumor size correlated inversely with irradiation dose, both in animals treated with CONV-RT and FLASH-RT. Acute dermal side effects were mild, but only a small proportion of the animals were alive for evaluation of those side effects.ConclusionThe dose response was similar for CONV-RT and FLASH-RT in the present model. Tumor size upon the time of euthanasia correlated inversely with the irradiation dose.https://www.frontiersin.org/articles/10.3389/fonc.2023.1309174/fullglioblastomaFLASH-RTCONV-RTtumor sizerat model |
| spellingShingle | Emma Liljedahl Elise Konradsson Karin Linderfalk Emma Gustafsson Kristoffer Petersson Kristoffer Petersson Crister Ceberg Henrietta Nittby Redebrandt Henrietta Nittby Redebrandt Comparable survival in rats with intracranial glioblastoma irradiated with single-fraction conventional radiotherapy or FLASH radiotherapy Frontiers in Oncology glioblastoma FLASH-RT CONV-RT tumor size rat model |
| title | Comparable survival in rats with intracranial glioblastoma irradiated with single-fraction conventional radiotherapy or FLASH radiotherapy |
| title_full | Comparable survival in rats with intracranial glioblastoma irradiated with single-fraction conventional radiotherapy or FLASH radiotherapy |
| title_fullStr | Comparable survival in rats with intracranial glioblastoma irradiated with single-fraction conventional radiotherapy or FLASH radiotherapy |
| title_full_unstemmed | Comparable survival in rats with intracranial glioblastoma irradiated with single-fraction conventional radiotherapy or FLASH radiotherapy |
| title_short | Comparable survival in rats with intracranial glioblastoma irradiated with single-fraction conventional radiotherapy or FLASH radiotherapy |
| title_sort | comparable survival in rats with intracranial glioblastoma irradiated with single fraction conventional radiotherapy or flash radiotherapy |
| topic | glioblastoma FLASH-RT CONV-RT tumor size rat model |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2023.1309174/full |
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