Fertility-Associated Polymorphism within Bovine <i>ITGβ5</i> and Its Significant Correlations with Ovarian and Luteal Traits
There is an urgent need to improve bovine fertility, and molecular marker-assisted selection (MAS) can accelerate this process. Genome-wide association studies suggest that <i>Integrin β5 (ITGβ5)</i> might affect fertility in bovines. As a member of the integrins family, ITGβ5 can bind t...
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MDPI AG
2021-05-01
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| author | Jianing Zhao Jie Li Fugui Jiang Enliang Song Xianyong Lan Haiyu Zhao |
| author_facet | Jianing Zhao Jie Li Fugui Jiang Enliang Song Xianyong Lan Haiyu Zhao |
| author_sort | Jianing Zhao |
| collection | DOAJ |
| description | There is an urgent need to improve bovine fertility, and molecular marker-assisted selection (MAS) can accelerate this process. Genome-wide association studies suggest that <i>Integrin β5 (ITGβ5)</i> might affect fertility in bovines. As a member of the integrins family, ITGβ5 can bind to the extracellular matrix and mediate various cellular processes. In our study, primers spanning six potential insertion/deletion (indel) polymorphisms within the <i>ITGβ5</i> gene were designed and 696 ovary samples from different individuals, the vast majority not in oestrum were collected for genetic variation detection. A deletion locus, rs522759246, namely P1-D<sub>13-bp</sub>, was found to be polymorphic. The allele D frequency was 0.152 and the polymorphism information content (PIC) value was 0.224, indicating a low-degree PIC. This locus did not follow the Hardy–Weinberg equilibrium (<i>p</i> = 1.200E-23). Importantly, associations between P1-D<sub>13-bp</sub> and ovarian morphological traits were established. Polymorphisms of this locus had significant correlations with ovarian width (<i>p</i> = 0.015). The corpus luteum is also linked to fertility and P1-D<sub>13-bp</sub> was significantly correlated with corpus luteum diameter (<i>p</i> = 0.005). In conclusion, an indel mutation within the bovine <i>ITGβ5</i> gene was identified, which was significantly associated with several ovarian and luteal traits. |
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| spelling | doaj.art-47cd9512c29c4aa2af537bbb0e13d02a2023-11-21T21:45:34ZengMDPI AGAnimals2076-26152021-05-01116157910.3390/ani11061579Fertility-Associated Polymorphism within Bovine <i>ITGβ5</i> and Its Significant Correlations with Ovarian and Luteal TraitsJianing Zhao0Jie Li1Fugui Jiang2Enliang Song3Xianyong Lan4Haiyu Zhao5School of Life Sciences, Lanzhou University, No. 222 South Tianshui Road, Lanzhou 730000, ChinaLaboratory of Animal Genome and Gene Function, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, ChinaShandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, ChinaShandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, ChinaLaboratory of Animal Genome and Gene Function, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, ChinaSchool of Life Sciences, Lanzhou University, No. 222 South Tianshui Road, Lanzhou 730000, ChinaThere is an urgent need to improve bovine fertility, and molecular marker-assisted selection (MAS) can accelerate this process. Genome-wide association studies suggest that <i>Integrin β5 (ITGβ5)</i> might affect fertility in bovines. As a member of the integrins family, ITGβ5 can bind to the extracellular matrix and mediate various cellular processes. In our study, primers spanning six potential insertion/deletion (indel) polymorphisms within the <i>ITGβ5</i> gene were designed and 696 ovary samples from different individuals, the vast majority not in oestrum were collected for genetic variation detection. A deletion locus, rs522759246, namely P1-D<sub>13-bp</sub>, was found to be polymorphic. The allele D frequency was 0.152 and the polymorphism information content (PIC) value was 0.224, indicating a low-degree PIC. This locus did not follow the Hardy–Weinberg equilibrium (<i>p</i> = 1.200E-23). Importantly, associations between P1-D<sub>13-bp</sub> and ovarian morphological traits were established. Polymorphisms of this locus had significant correlations with ovarian width (<i>p</i> = 0.015). The corpus luteum is also linked to fertility and P1-D<sub>13-bp</sub> was significantly correlated with corpus luteum diameter (<i>p</i> = 0.005). In conclusion, an indel mutation within the bovine <i>ITGβ5</i> gene was identified, which was significantly associated with several ovarian and luteal traits.https://www.mdpi.com/2076-2615/11/6/1579bovine<i>ITGβ5</i> geneinsertion/deletion (indel)ovarian traitscorpus luteum |
| spellingShingle | Jianing Zhao Jie Li Fugui Jiang Enliang Song Xianyong Lan Haiyu Zhao Fertility-Associated Polymorphism within Bovine <i>ITGβ5</i> and Its Significant Correlations with Ovarian and Luteal Traits Animals bovine <i>ITGβ5</i> gene insertion/deletion (indel) ovarian traits corpus luteum |
| title | Fertility-Associated Polymorphism within Bovine <i>ITGβ5</i> and Its Significant Correlations with Ovarian and Luteal Traits |
| title_full | Fertility-Associated Polymorphism within Bovine <i>ITGβ5</i> and Its Significant Correlations with Ovarian and Luteal Traits |
| title_fullStr | Fertility-Associated Polymorphism within Bovine <i>ITGβ5</i> and Its Significant Correlations with Ovarian and Luteal Traits |
| title_full_unstemmed | Fertility-Associated Polymorphism within Bovine <i>ITGβ5</i> and Its Significant Correlations with Ovarian and Luteal Traits |
| title_short | Fertility-Associated Polymorphism within Bovine <i>ITGβ5</i> and Its Significant Correlations with Ovarian and Luteal Traits |
| title_sort | fertility associated polymorphism within bovine i itgβ5 i and its significant correlations with ovarian and luteal traits |
| topic | bovine <i>ITGβ5</i> gene insertion/deletion (indel) ovarian traits corpus luteum |
| url | https://www.mdpi.com/2076-2615/11/6/1579 |
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