On genetic diversity in caraway: Genotyping of a large germplasm collection.
Caraway (Carum carvi) is a widespread and frequently used spice and medicinal plant with a long history of cultivation. However, due to ongoing climatic changes, the cultivation is becoming increasingly risky. To secure caraway cultivation in future, timely breeding efforts to develop adapted materi...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2020-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0244666 |
| _version_ | 1819140705717583872 |
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| author | Daniel von Maydell Heike Lehnert Thomas Berner Evelyn Klocke Wolfram Junghanns Jens Keilwagen Frank Marthe |
| author_facet | Daniel von Maydell Heike Lehnert Thomas Berner Evelyn Klocke Wolfram Junghanns Jens Keilwagen Frank Marthe |
| author_sort | Daniel von Maydell |
| collection | DOAJ |
| description | Caraway (Carum carvi) is a widespread and frequently used spice and medicinal plant with a long history of cultivation. However, due to ongoing climatic changes, the cultivation is becoming increasingly risky. To secure caraway cultivation in future, timely breeding efforts to develop adapted material are necessary. Analysis of genetic diversity can accompany this process, for instance, by revealing untapped gene pools. Here, we analyzed 137 accessions using genotyping by sequencing (GBS). Hence, we can report a broad overview of population structure and genetic diversity of caraway. Population structure was determined using a principal coordinate analysis, a Bayesian clustering analysis, phylogenetic trees and a neighbor network based on 13,155 SNPs. Genotypic data indicate a clear separation of accessions into two subpopulations, which correlates with the flowering type (annual vs. biennial). Four winter-annual accessions were closer related to biennial accessions. In an analysis of molecular variance, genetic variation between the two subpopulations was 7.84%. In addition, we estimated the genome size for 35 accessions by flow cytometry. An average genome size of 4.282 pg/2C (± 0.0096 S.E.) was estimated. Therefore, we suggest a significantly smaller genome size than stated in literature. |
| first_indexed | 2024-12-22T11:42:48Z |
| format | Article |
| id | doaj.art-47d1b2a8a00743d6960b1f295b444c2a |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T11:42:48Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-47d1b2a8a00743d6960b1f295b444c2a2022-12-21T18:27:13ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-011512e024466610.1371/journal.pone.0244666On genetic diversity in caraway: Genotyping of a large germplasm collection.Daniel von MaydellHeike LehnertThomas BernerEvelyn KlockeWolfram JunghannsJens KeilwagenFrank MartheCaraway (Carum carvi) is a widespread and frequently used spice and medicinal plant with a long history of cultivation. However, due to ongoing climatic changes, the cultivation is becoming increasingly risky. To secure caraway cultivation in future, timely breeding efforts to develop adapted material are necessary. Analysis of genetic diversity can accompany this process, for instance, by revealing untapped gene pools. Here, we analyzed 137 accessions using genotyping by sequencing (GBS). Hence, we can report a broad overview of population structure and genetic diversity of caraway. Population structure was determined using a principal coordinate analysis, a Bayesian clustering analysis, phylogenetic trees and a neighbor network based on 13,155 SNPs. Genotypic data indicate a clear separation of accessions into two subpopulations, which correlates with the flowering type (annual vs. biennial). Four winter-annual accessions were closer related to biennial accessions. In an analysis of molecular variance, genetic variation between the two subpopulations was 7.84%. In addition, we estimated the genome size for 35 accessions by flow cytometry. An average genome size of 4.282 pg/2C (± 0.0096 S.E.) was estimated. Therefore, we suggest a significantly smaller genome size than stated in literature.https://doi.org/10.1371/journal.pone.0244666 |
| spellingShingle | Daniel von Maydell Heike Lehnert Thomas Berner Evelyn Klocke Wolfram Junghanns Jens Keilwagen Frank Marthe On genetic diversity in caraway: Genotyping of a large germplasm collection. PLoS ONE |
| title | On genetic diversity in caraway: Genotyping of a large germplasm collection. |
| title_full | On genetic diversity in caraway: Genotyping of a large germplasm collection. |
| title_fullStr | On genetic diversity in caraway: Genotyping of a large germplasm collection. |
| title_full_unstemmed | On genetic diversity in caraway: Genotyping of a large germplasm collection. |
| title_short | On genetic diversity in caraway: Genotyping of a large germplasm collection. |
| title_sort | on genetic diversity in caraway genotyping of a large germplasm collection |
| url | https://doi.org/10.1371/journal.pone.0244666 |
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