Factors Associated With the Microbiome in Moderate–Late Preterm Babies: A Cohort Study From the DIAMOND Randomized Controlled Trial
The gut microbiota of preterm infants is affected by perinatal factors and, in turn, may impact upon infant health. In this study, we collected fecal samples at Day-10 (D10) and 4-months corrected-age (4M) from 227 moderate–late preterm (MLPT) babies enrolled in a randomized controlled trial of nutr...
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| Language: | English |
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Frontiers Media S.A.
2021-03-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2021.595323/full |
| _version_ | 1818617609512288256 |
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| author | Clara Yieh Lin Chong Tommi Vatanen Tommi Vatanen Tanith Alexander Tanith Alexander Frank H. Bloomfield Justin M. O’Sullivan Justin M. O’Sullivan |
| author_facet | Clara Yieh Lin Chong Tommi Vatanen Tommi Vatanen Tanith Alexander Tanith Alexander Frank H. Bloomfield Justin M. O’Sullivan Justin M. O’Sullivan |
| author_sort | Clara Yieh Lin Chong |
| collection | DOAJ |
| description | The gut microbiota of preterm infants is affected by perinatal factors and, in turn, may impact upon infant health. In this study, we collected fecal samples at Day-10 (D10) and 4-months corrected-age (4M) from 227 moderate–late preterm (MLPT) babies enrolled in a randomized controlled trial of nutritional management. A total of 320 samples underwent 16S amplicon sequencing, and shotgun metagenomic sequencing was performed on 94 samples from the 4M time point. The microbiome of babies whose families lived in lower socioeconomic status (SES) areas exhibited a significantly higher microbial alpha diversity at D10 (Wilcoxon test, p = 0.021), greater abundance of Bifidobacterium (linear model, q = 0.020) at D10 and Megasphaera (q = 0.031) at 4M. Hospital of birth explained 5.2% of the observed variance in 4M samples (PERMANOVA, p = 0.038), with Staphylococcus aureus more abundant in fecal samples from babies born in Middlemore hospital (linear model, q = 0.016). Maternal antibiotic (Wilcoxon test, p = 0.013) and probiotic (p = 0.04) usage within the four-week period before sample collection was associated with a reduction in the alpha diversity of D10 samples. Infant probiotic intake explained 2.1% (PERMANOVA, p = 0.021) of the variance in the D10 microbial profile with increased Lactobacillus (linear model, q = 1.1 × 10−10) levels. At 4M, the microbiome of infants who were breastmilk fed had reduced alpha diversity when compared to non-breastmilk fed infants (Wilcoxon test, p < 0.05). Although causality cannot be inferred within our study, we conclude that in MLPT babies, maternal socioeconomic factors, as well as the perinatal medical environment and nutrition impact on the development of the newborn microbiome. |
| first_indexed | 2024-12-16T17:08:25Z |
| format | Article |
| id | doaj.art-47d325ad27e74669a6b9058e69064ff3 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-16T17:08:25Z |
| publishDate | 2021-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-47d325ad27e74669a6b9058e69064ff32022-12-21T22:23:29ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882021-03-011110.3389/fcimb.2021.595323595323Factors Associated With the Microbiome in Moderate–Late Preterm Babies: A Cohort Study From the DIAMOND Randomized Controlled TrialClara Yieh Lin Chong0Tommi Vatanen1Tommi Vatanen2Tanith Alexander3Tanith Alexander4Frank H. Bloomfield5Justin M. O’Sullivan6Justin M. O’Sullivan7Liggins Institute, The University of Auckland, Auckland, New ZealandLiggins Institute, The University of Auckland, Auckland, New ZealandInfectious Disease & Microbiome Program, The Broad Institute of MIT and Harvard, Cambridge, MA, United StatesLiggins Institute, The University of Auckland, Auckland, New ZealandNeonatal Unit, Kidz First, Middlemore Hospital, Auckland, New ZealandLiggins Institute, The University of Auckland, Auckland, New ZealandLiggins Institute, The University of Auckland, Auckland, New ZealandThe Maurice Wilkins Centre, The University of Auckland, Auckland, New ZealandThe gut microbiota of preterm infants is affected by perinatal factors and, in turn, may impact upon infant health. In this study, we collected fecal samples at Day-10 (D10) and 4-months corrected-age (4M) from 227 moderate–late preterm (MLPT) babies enrolled in a randomized controlled trial of nutritional management. A total of 320 samples underwent 16S amplicon sequencing, and shotgun metagenomic sequencing was performed on 94 samples from the 4M time point. The microbiome of babies whose families lived in lower socioeconomic status (SES) areas exhibited a significantly higher microbial alpha diversity at D10 (Wilcoxon test, p = 0.021), greater abundance of Bifidobacterium (linear model, q = 0.020) at D10 and Megasphaera (q = 0.031) at 4M. Hospital of birth explained 5.2% of the observed variance in 4M samples (PERMANOVA, p = 0.038), with Staphylococcus aureus more abundant in fecal samples from babies born in Middlemore hospital (linear model, q = 0.016). Maternal antibiotic (Wilcoxon test, p = 0.013) and probiotic (p = 0.04) usage within the four-week period before sample collection was associated with a reduction in the alpha diversity of D10 samples. Infant probiotic intake explained 2.1% (PERMANOVA, p = 0.021) of the variance in the D10 microbial profile with increased Lactobacillus (linear model, q = 1.1 × 10−10) levels. At 4M, the microbiome of infants who were breastmilk fed had reduced alpha diversity when compared to non-breastmilk fed infants (Wilcoxon test, p < 0.05). Although causality cannot be inferred within our study, we conclude that in MLPT babies, maternal socioeconomic factors, as well as the perinatal medical environment and nutrition impact on the development of the newborn microbiome.https://www.frontiersin.org/articles/10.3389/fcimb.2021.595323/fullmoderate–late preterm infantsocioeconomic statusethnicitygut microbiomeearly life nutrition |
| spellingShingle | Clara Yieh Lin Chong Tommi Vatanen Tommi Vatanen Tanith Alexander Tanith Alexander Frank H. Bloomfield Justin M. O’Sullivan Justin M. O’Sullivan Factors Associated With the Microbiome in Moderate–Late Preterm Babies: A Cohort Study From the DIAMOND Randomized Controlled Trial Frontiers in Cellular and Infection Microbiology moderate–late preterm infant socioeconomic status ethnicity gut microbiome early life nutrition |
| title | Factors Associated With the Microbiome in Moderate–Late Preterm Babies: A Cohort Study From the DIAMOND Randomized Controlled Trial |
| title_full | Factors Associated With the Microbiome in Moderate–Late Preterm Babies: A Cohort Study From the DIAMOND Randomized Controlled Trial |
| title_fullStr | Factors Associated With the Microbiome in Moderate–Late Preterm Babies: A Cohort Study From the DIAMOND Randomized Controlled Trial |
| title_full_unstemmed | Factors Associated With the Microbiome in Moderate–Late Preterm Babies: A Cohort Study From the DIAMOND Randomized Controlled Trial |
| title_short | Factors Associated With the Microbiome in Moderate–Late Preterm Babies: A Cohort Study From the DIAMOND Randomized Controlled Trial |
| title_sort | factors associated with the microbiome in moderate late preterm babies a cohort study from the diamond randomized controlled trial |
| topic | moderate–late preterm infant socioeconomic status ethnicity gut microbiome early life nutrition |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2021.595323/full |
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