Adult murine skeletal muscle contains cells that can differentiate into beating cardiomyocytes in vitro.
It has long been held as scientific fact that soon after birth, cardiomyocytes cease dividing, thus explaining the limited restoration of cardiac function after a heart attack. Recent demonstrations of cardiac myocyte differentiation observed in vitro or after in vivo transplantation of adult stem c...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2005-04-01
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| Series: | PLoS Biology |
| Online Access: | http://europepmc.org/articles/PMC1064849?pdf=render |
| _version_ | 1818882858448584704 |
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| author | Steve O Winitsky Thiru V Gopal Shahin Hassanzadeh Hiroshi Takahashi Divina Gryder Michael A Rogawski Kazuyo Takeda Zu X Yu Yu H Xu Neal D Epstein |
| author_facet | Steve O Winitsky Thiru V Gopal Shahin Hassanzadeh Hiroshi Takahashi Divina Gryder Michael A Rogawski Kazuyo Takeda Zu X Yu Yu H Xu Neal D Epstein |
| author_sort | Steve O Winitsky |
| collection | DOAJ |
| description | It has long been held as scientific fact that soon after birth, cardiomyocytes cease dividing, thus explaining the limited restoration of cardiac function after a heart attack. Recent demonstrations of cardiac myocyte differentiation observed in vitro or after in vivo transplantation of adult stem cells from blood, fat, skeletal muscle, or heart have challenged this view. Analysis of these studies has been complicated by the large disparity in the magnitude of effects seen by different groups and obscured by the recently appreciated process of in vivo stem-cell fusion. We now show a novel population of nonsatellite cells in adult murine skeletal muscle that progress under standard primary cell-culture conditions to autonomously beating cardiomyocytes. Their differentiation into beating cardiomyocytes is characterized here by video microscopy, confocal-detected calcium transients, electron microscopy, immunofluorescent cardiac-specific markers, and single-cell patch recordings of cardiac action potentials. Within 2 d after tail-vein injection of these marked cells into a mouse model of acute infarction, the marked cells are visible in the heart. By 6 d they begin to differentiate without fusing to recipient cardiac cells. Three months later, the tagged cells are visible as striated heart muscle restricted to the region of the cardiac infarct. |
| first_indexed | 2024-12-19T15:24:26Z |
| format | Article |
| id | doaj.art-47d3a49c068f4bd0bbd19656f2571872 |
| institution | Directory Open Access Journal |
| issn | 1544-9173 1545-7885 |
| language | English |
| last_indexed | 2024-12-19T15:24:26Z |
| publishDate | 2005-04-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Biology |
| spelling | doaj.art-47d3a49c068f4bd0bbd19656f25718722022-12-21T20:15:55ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852005-04-0134e8710.1371/journal.pbio.0030087Adult murine skeletal muscle contains cells that can differentiate into beating cardiomyocytes in vitro.Steve O WinitskyThiru V GopalShahin HassanzadehHiroshi TakahashiDivina GryderMichael A RogawskiKazuyo TakedaZu X YuYu H XuNeal D EpsteinIt has long been held as scientific fact that soon after birth, cardiomyocytes cease dividing, thus explaining the limited restoration of cardiac function after a heart attack. Recent demonstrations of cardiac myocyte differentiation observed in vitro or after in vivo transplantation of adult stem cells from blood, fat, skeletal muscle, or heart have challenged this view. Analysis of these studies has been complicated by the large disparity in the magnitude of effects seen by different groups and obscured by the recently appreciated process of in vivo stem-cell fusion. We now show a novel population of nonsatellite cells in adult murine skeletal muscle that progress under standard primary cell-culture conditions to autonomously beating cardiomyocytes. Their differentiation into beating cardiomyocytes is characterized here by video microscopy, confocal-detected calcium transients, electron microscopy, immunofluorescent cardiac-specific markers, and single-cell patch recordings of cardiac action potentials. Within 2 d after tail-vein injection of these marked cells into a mouse model of acute infarction, the marked cells are visible in the heart. By 6 d they begin to differentiate without fusing to recipient cardiac cells. Three months later, the tagged cells are visible as striated heart muscle restricted to the region of the cardiac infarct.http://europepmc.org/articles/PMC1064849?pdf=render |
| spellingShingle | Steve O Winitsky Thiru V Gopal Shahin Hassanzadeh Hiroshi Takahashi Divina Gryder Michael A Rogawski Kazuyo Takeda Zu X Yu Yu H Xu Neal D Epstein Adult murine skeletal muscle contains cells that can differentiate into beating cardiomyocytes in vitro. PLoS Biology |
| title | Adult murine skeletal muscle contains cells that can differentiate into beating cardiomyocytes in vitro. |
| title_full | Adult murine skeletal muscle contains cells that can differentiate into beating cardiomyocytes in vitro. |
| title_fullStr | Adult murine skeletal muscle contains cells that can differentiate into beating cardiomyocytes in vitro. |
| title_full_unstemmed | Adult murine skeletal muscle contains cells that can differentiate into beating cardiomyocytes in vitro. |
| title_short | Adult murine skeletal muscle contains cells that can differentiate into beating cardiomyocytes in vitro. |
| title_sort | adult murine skeletal muscle contains cells that can differentiate into beating cardiomyocytes in vitro |
| url | http://europepmc.org/articles/PMC1064849?pdf=render |
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