Transcriptomic and metabolomic reveals silicon enhances adaptation of rice under dry cultivation by improving flavonoid biosynthesis, osmoregulation, and photosynthesis
Dry cultivation is a new rice crop mode used to alleviate water shortage and develop water-saving agriculture. There is obvious genetic difference compared with drought-tolerant rice. Silicon (Si) plays an important role in plant adaptation to adverse environmental conditions and can significantly i...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.967537/full |
| _version_ | 1828519754451648512 |
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| author | Hao Jiang Ze Song Qing-Wang Su Zhi-Heng Wei Wan-Chun Li Zi-Xian Jiang Ping Tian Zhen-Hui Wang Xue Yang Mei-Ying Yang Xiao-Shuang Wei Zhi-Hai Wu Zhi-Hai Wu |
| author_facet | Hao Jiang Ze Song Qing-Wang Su Zhi-Heng Wei Wan-Chun Li Zi-Xian Jiang Ping Tian Zhen-Hui Wang Xue Yang Mei-Ying Yang Xiao-Shuang Wei Zhi-Hai Wu Zhi-Hai Wu |
| author_sort | Hao Jiang |
| collection | DOAJ |
| description | Dry cultivation is a new rice crop mode used to alleviate water shortage and develop water-saving agriculture. There is obvious genetic difference compared with drought-tolerant rice. Silicon (Si) plays an important role in plant adaptation to adverse environmental conditions and can significantly improve the drought tolerance and yield of rice. However, the regulatory mechanism via which Si provides plant tolerance or adaptation under dry cultivation is not well understood. The present study investigated the changes in plant growth, photosynthetic gas exchange, and oxidative stress of the rice cultivar “Suijing 18” under dry cultivation. Si improved photosynthetic performance and antioxidant enzyme activity and subsequently reduced lipid peroxidation of rice seedlings, promoted LAI and promoted leaf growth under dry cultivation. Further, transcriptomics combined with quasi-targeted metabolomics detected 1416 and 520 differentially expressed genes (DEGs), 38 and 41 differentially accumulated metabolites (DAMs) in the rice leaves and roots, respectively. Among them, 13 DEGs were involved in flavonoid biosynthesis, promoting the accumulation of flavonoids, anthocyanins, and flavonols in the roots and leaves of rice under dry cultivation. Meanwhile, 14 DEGs were involved in photosynthesis, promoting photosystem I and photosystem II responses, increasing the abundance of metabolites in leaves. On the other hand, 24 DAMs were identified involved in osmoregulatory processes, significantly increasing amino acids and carbohydrates and their derivatives in roots. These results provide new insight into the role of Si in alleviating to adverse environmental, Si enhanced the accumulation of flavonoids and osmoregulatory metabolites, thereby alleviating drought effect on the roots. On the other hand, improving dehydration resistance of leaves, guaranteeing normal photosynthesis and downward transport of organic matter. In conclusion, Si promoted the coordinated action between the above-ground and below-ground plant parts, improved the root/shoot ratio (R/S) of rice and increased the sugar content and enhancing rice adaptability under dry cultivation conditions. The establishment of the system for increasing the yield of rice under dry cultivation provides theoretical and technical support thereby promoting the rapid development of rice in Northeast China, and ensuring national food security. |
| first_indexed | 2024-12-11T19:17:40Z |
| format | Article |
| id | doaj.art-47d9f8c946374475bd64b07856b94d68 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-11T19:17:40Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-47d9f8c946374475bd64b07856b94d682022-12-22T00:53:36ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-08-011310.3389/fpls.2022.967537967537Transcriptomic and metabolomic reveals silicon enhances adaptation of rice under dry cultivation by improving flavonoid biosynthesis, osmoregulation, and photosynthesisHao Jiang0Ze Song1Qing-Wang Su2Zhi-Heng Wei3Wan-Chun Li4Zi-Xian Jiang5Ping Tian6Zhen-Hui Wang7Xue Yang8Mei-Ying Yang9Xiao-Shuang Wei10Zhi-Hai Wu11Zhi-Hai Wu12College of Agronomy, Jilin Agricultural University, Changchun, ChinaCollege of Agronomy, Jilin Agricultural University, Changchun, ChinaCollege of Agronomy, Jilin Agricultural University, Changchun, ChinaCollege of Agronomy, Jilin Agricultural University, Changchun, ChinaCollege of Agronomy, Jilin Agricultural University, Changchun, ChinaCollege of Agronomy, Jilin Agricultural University, Changchun, ChinaCollege of Agronomy, Jilin Agricultural University, Changchun, ChinaCollege of Agronomy, Jilin Agricultural University, Changchun, ChinaCollege of Life Sciences, Jilin Agricultural University, Changchun, ChinaCollege of Life Sciences, Jilin Agricultural University, Changchun, ChinaCollege of Agronomy, Jilin Agricultural University, Changchun, ChinaCollege of Agronomy, Jilin Agricultural University, Changchun, ChinaNational Crop Variety Approval and Characteristic Identification Station, Jilin Agricultural University, Changchun, ChinaDry cultivation is a new rice crop mode used to alleviate water shortage and develop water-saving agriculture. There is obvious genetic difference compared with drought-tolerant rice. Silicon (Si) plays an important role in plant adaptation to adverse environmental conditions and can significantly improve the drought tolerance and yield of rice. However, the regulatory mechanism via which Si provides plant tolerance or adaptation under dry cultivation is not well understood. The present study investigated the changes in plant growth, photosynthetic gas exchange, and oxidative stress of the rice cultivar “Suijing 18” under dry cultivation. Si improved photosynthetic performance and antioxidant enzyme activity and subsequently reduced lipid peroxidation of rice seedlings, promoted LAI and promoted leaf growth under dry cultivation. Further, transcriptomics combined with quasi-targeted metabolomics detected 1416 and 520 differentially expressed genes (DEGs), 38 and 41 differentially accumulated metabolites (DAMs) in the rice leaves and roots, respectively. Among them, 13 DEGs were involved in flavonoid biosynthesis, promoting the accumulation of flavonoids, anthocyanins, and flavonols in the roots and leaves of rice under dry cultivation. Meanwhile, 14 DEGs were involved in photosynthesis, promoting photosystem I and photosystem II responses, increasing the abundance of metabolites in leaves. On the other hand, 24 DAMs were identified involved in osmoregulatory processes, significantly increasing amino acids and carbohydrates and their derivatives in roots. These results provide new insight into the role of Si in alleviating to adverse environmental, Si enhanced the accumulation of flavonoids and osmoregulatory metabolites, thereby alleviating drought effect on the roots. On the other hand, improving dehydration resistance of leaves, guaranteeing normal photosynthesis and downward transport of organic matter. In conclusion, Si promoted the coordinated action between the above-ground and below-ground plant parts, improved the root/shoot ratio (R/S) of rice and increased the sugar content and enhancing rice adaptability under dry cultivation conditions. The establishment of the system for increasing the yield of rice under dry cultivation provides theoretical and technical support thereby promoting the rapid development of rice in Northeast China, and ensuring national food security.https://www.frontiersin.org/articles/10.3389/fpls.2022.967537/fullricedry cultivationtranscriptomicquasi-targeted metabolomicsphotosynthesisosmoregulatory |
| spellingShingle | Hao Jiang Ze Song Qing-Wang Su Zhi-Heng Wei Wan-Chun Li Zi-Xian Jiang Ping Tian Zhen-Hui Wang Xue Yang Mei-Ying Yang Xiao-Shuang Wei Zhi-Hai Wu Zhi-Hai Wu Transcriptomic and metabolomic reveals silicon enhances adaptation of rice under dry cultivation by improving flavonoid biosynthesis, osmoregulation, and photosynthesis Frontiers in Plant Science rice dry cultivation transcriptomic quasi-targeted metabolomics photosynthesis osmoregulatory |
| title | Transcriptomic and metabolomic reveals silicon enhances adaptation of rice under dry cultivation by improving flavonoid biosynthesis, osmoregulation, and photosynthesis |
| title_full | Transcriptomic and metabolomic reveals silicon enhances adaptation of rice under dry cultivation by improving flavonoid biosynthesis, osmoregulation, and photosynthesis |
| title_fullStr | Transcriptomic and metabolomic reveals silicon enhances adaptation of rice under dry cultivation by improving flavonoid biosynthesis, osmoregulation, and photosynthesis |
| title_full_unstemmed | Transcriptomic and metabolomic reveals silicon enhances adaptation of rice under dry cultivation by improving flavonoid biosynthesis, osmoregulation, and photosynthesis |
| title_short | Transcriptomic and metabolomic reveals silicon enhances adaptation of rice under dry cultivation by improving flavonoid biosynthesis, osmoregulation, and photosynthesis |
| title_sort | transcriptomic and metabolomic reveals silicon enhances adaptation of rice under dry cultivation by improving flavonoid biosynthesis osmoregulation and photosynthesis |
| topic | rice dry cultivation transcriptomic quasi-targeted metabolomics photosynthesis osmoregulatory |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.967537/full |
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