Capping protein regulates endosomal trafficking by controlling F-actin density around endocytic vesicles and recruiting RAB5 effectors
Actin filaments (F-actin) have been implicated in various steps of endosomal trafficking, and the length of F-actin is controlled by actin capping proteins, such as CapZ, which is a stable heterodimeric protein complex consisting of α and β subunits. However, the role of these capping proteins in en...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2021-11-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/65910 |
| _version_ | 1811180473113640960 |
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| author | Dawei Wang Zuodong Ye Wenjie Wei Jingting Yu Lihong Huang Hongmin Zhang Jianbo Yue |
| author_facet | Dawei Wang Zuodong Ye Wenjie Wei Jingting Yu Lihong Huang Hongmin Zhang Jianbo Yue |
| author_sort | Dawei Wang |
| collection | DOAJ |
| description | Actin filaments (F-actin) have been implicated in various steps of endosomal trafficking, and the length of F-actin is controlled by actin capping proteins, such as CapZ, which is a stable heterodimeric protein complex consisting of α and β subunits. However, the role of these capping proteins in endosomal trafficking remains elusive. Here, we found that CapZ docks to endocytic vesicles via its C-terminal actin-binding motif. CapZ knockout significantly increases the F-actin density around immature early endosomes, and this impedes fusion between these vesicles, manifested by the accumulation of small endocytic vesicles in CapZ-knockout cells. CapZ also recruits several RAB5 effectors, such as Rabaptin-5 and Rabex-5, to RAB5-positive early endosomes via its N-terminal domain, and this further activates RAB5. Collectively, our results indicate that CapZ regulates endosomal trafficking by controlling actin density around early endosomes and recruiting RAB5 effectors. |
| first_indexed | 2024-04-11T09:03:41Z |
| format | Article |
| id | doaj.art-47da48c69a5e46f49d024ae0ed8bc565 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T09:03:41Z |
| publishDate | 2021-11-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-47da48c69a5e46f49d024ae0ed8bc5652022-12-22T04:32:42ZengeLife Sciences Publications LtdeLife2050-084X2021-11-011010.7554/eLife.65910Capping protein regulates endosomal trafficking by controlling F-actin density around endocytic vesicles and recruiting RAB5 effectorsDawei Wang0https://orcid.org/0000-0002-7868-775XZuodong Ye1Wenjie Wei2Jingting Yu3https://orcid.org/0000-0002-2631-8434Lihong Huang4Hongmin Zhang5https://orcid.org/0000-0003-4356-3615Jianbo Yue6https://orcid.org/0000-0001-6384-5447City University of Hong Kong Shenzhen Research Institute, Shenzhen, China; Department of Biomedical Sciences, City University of Hong Kong, Hong Kong, ChinaCity University of Hong Kong Shenzhen Research Institute, Shenzhen, China; Department of Biomedical Sciences, City University of Hong Kong, Hong Kong, ChinaCore Research Facilities, Southern University of Science and Technology, Shenzhen, ChinaCity University of Hong Kong Shenzhen Research Institute, Shenzhen, ChinaCity University of Hong Kong Shenzhen Research Institute, Shenzhen, China; Department of Biomedical Sciences, City University of Hong Kong, Hong Kong, ChinaDepartment of Biology, Guangdong Provincial Key Laboratory of Cell Microenvironment and Disease Research and Shenzhen Key Laboratory of Cell Microenvironment, Southern University of Science and Technology, Shenzhen, ChinaCity University of Hong Kong Shenzhen Research Institute, Shenzhen, China; Department of Biomedical Sciences, City University of Hong Kong, Hong Kong, China; City University of Hong Kong Chengdu Research Institute, Chengdu, ChinaActin filaments (F-actin) have been implicated in various steps of endosomal trafficking, and the length of F-actin is controlled by actin capping proteins, such as CapZ, which is a stable heterodimeric protein complex consisting of α and β subunits. However, the role of these capping proteins in endosomal trafficking remains elusive. Here, we found that CapZ docks to endocytic vesicles via its C-terminal actin-binding motif. CapZ knockout significantly increases the F-actin density around immature early endosomes, and this impedes fusion between these vesicles, manifested by the accumulation of small endocytic vesicles in CapZ-knockout cells. CapZ also recruits several RAB5 effectors, such as Rabaptin-5 and Rabex-5, to RAB5-positive early endosomes via its N-terminal domain, and this further activates RAB5. Collectively, our results indicate that CapZ regulates endosomal trafficking by controlling actin density around early endosomes and recruiting RAB5 effectors.https://elifesciences.org/articles/65910CapZF-actinendosomeRAB5endosomal traffickingRabaptin-5 |
| spellingShingle | Dawei Wang Zuodong Ye Wenjie Wei Jingting Yu Lihong Huang Hongmin Zhang Jianbo Yue Capping protein regulates endosomal trafficking by controlling F-actin density around endocytic vesicles and recruiting RAB5 effectors eLife CapZ F-actin endosome RAB5 endosomal trafficking Rabaptin-5 |
| title | Capping protein regulates endosomal trafficking by controlling F-actin density around endocytic vesicles and recruiting RAB5 effectors |
| title_full | Capping protein regulates endosomal trafficking by controlling F-actin density around endocytic vesicles and recruiting RAB5 effectors |
| title_fullStr | Capping protein regulates endosomal trafficking by controlling F-actin density around endocytic vesicles and recruiting RAB5 effectors |
| title_full_unstemmed | Capping protein regulates endosomal trafficking by controlling F-actin density around endocytic vesicles and recruiting RAB5 effectors |
| title_short | Capping protein regulates endosomal trafficking by controlling F-actin density around endocytic vesicles and recruiting RAB5 effectors |
| title_sort | capping protein regulates endosomal trafficking by controlling f actin density around endocytic vesicles and recruiting rab5 effectors |
| topic | CapZ F-actin endosome RAB5 endosomal trafficking Rabaptin-5 |
| url | https://elifesciences.org/articles/65910 |
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