Efecto de las PGPB sobre el crecimiento Pennisetum clandestinum bajo condiciones de estrés salino
This research aims the isolation and characterization of bacteria with potential to promote the growth of grass Pennisetum clandestinum in simulated saline soils. 92 Gram positive bacterias were isolated from Rhizosphere soil samples of P. clandestinum. Ten isolated bacteria grew on nutrient agar su...
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Format: | Article |
Language: | Spanish |
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Universidad Nacional de Colombia
2016-05-01
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Series: | Revista Colombiana de Biotecnología |
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Online Access: | http://www.revistas.unal.edu.co/index.php/biotecnologia/article/view/50413 |
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author | Diana Beatriz Sánchez López Jazmín Vanessa Pérez Pazos Hilda Adriana David Hinestroza |
author_facet | Diana Beatriz Sánchez López Jazmín Vanessa Pérez Pazos Hilda Adriana David Hinestroza |
author_sort | Diana Beatriz Sánchez López |
collection | DOAJ |
description | This research aims the isolation and characterization of bacteria with potential to promote the growth of grass Pennisetum clandestinum in simulated saline soils. 92 Gram positive bacterias were isolated from Rhizosphere soil samples of P. clandestinum. Ten isolated bacteria grew on nutrient agar supplemented with NaCl (2.578 M). Isolates were evaluated under greenhouse conditions; the strains identified as KISA 34 and KISA 71 were selected as the best based on the statistical test of Dunnet (p ≤ 0.05) and were identified Bacillus amyloliquefaciens KISA 34 and Bacillus subtilis KISA 71. These strains have the ability to produce ammonium, exopolysaccharides and cellulose, both in absence and presence of NaCl. The strains produced indoles and phosphorus solubilization. The evaluation of strains in greenhouse showed that the T6 - KISA 34 + KISA 71+ 25 % (phosphate rock RF) significantly increased biomass and plant development (p≤ 0.05) compared with T2 - full fertilization . The results of this research showed that isolates have the ability to grow in saline soils retaining its characteristics as promoting plant growth with positive effects on P. clandestinum. |
first_indexed | 2024-12-23T19:17:29Z |
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id | doaj.art-47dc43675ca242d29ec737d015fb0ea6 |
institution | Directory Open Access Journal |
issn | 0123-3475 1909-8758 |
language | Spanish |
last_indexed | 2024-12-23T19:17:29Z |
publishDate | 2016-05-01 |
publisher | Universidad Nacional de Colombia |
record_format | Article |
series | Revista Colombiana de Biotecnología |
spelling | doaj.art-47dc43675ca242d29ec737d015fb0ea62022-12-21T17:34:17ZspaUniversidad Nacional de ColombiaRevista Colombiana de Biotecnología0123-34751909-87582016-05-011816572Efecto de las PGPB sobre el crecimiento Pennisetum clandestinum bajo condiciones de estrés salinoDiana Beatriz Sánchez López0Jazmín Vanessa Pérez Pazos1Hilda Adriana David Hinestroza2M.Sc. Ciencias Biológicas. Centro de investigación Turipaná. Corporación Colombiana de Investigación Agropecuaria (Corpoica). km 13 vía Montería-Cereté, Colombia. Telephone +57(1) 4227300 Ext. 2249. E-mail correspondencia: dbsanchez@corpoica.org.coB.Sc Bióloga. Centro de investigación Turipaná. Corporación Colombiana de Investigación Agropecuaria (Corpoica). km 13 vía Montería-Cereté, Colombia. Telephone +57(1) 4227300 Ext. 2249. jvperez@corpoica.org.coM.Sc. Nutrición de Rumiantes. Centro de investigación Turipaná. Corporación Colombiana de Investigación Agropecuaria (Corpoica). km 13 vía Montería -Cereté, Colombia. adavid@corpoica.org.coThis research aims the isolation and characterization of bacteria with potential to promote the growth of grass Pennisetum clandestinum in simulated saline soils. 92 Gram positive bacterias were isolated from Rhizosphere soil samples of P. clandestinum. Ten isolated bacteria grew on nutrient agar supplemented with NaCl (2.578 M). Isolates were evaluated under greenhouse conditions; the strains identified as KISA 34 and KISA 71 were selected as the best based on the statistical test of Dunnet (p ≤ 0.05) and were identified Bacillus amyloliquefaciens KISA 34 and Bacillus subtilis KISA 71. These strains have the ability to produce ammonium, exopolysaccharides and cellulose, both in absence and presence of NaCl. The strains produced indoles and phosphorus solubilization. The evaluation of strains in greenhouse showed that the T6 - KISA 34 + KISA 71+ 25 % (phosphate rock RF) significantly increased biomass and plant development (p≤ 0.05) compared with T2 - full fertilization . The results of this research showed that isolates have the ability to grow in saline soils retaining its characteristics as promoting plant growth with positive effects on P. clandestinum.http://www.revistas.unal.edu.co/index.php/biotecnologia/article/view/50413Bacillus subtilisBacillus amyloliquefaciensbiofertilizantessalinidadsolubilización de fósforobiofertilizersalinityphosphorus solubilization |
spellingShingle | Diana Beatriz Sánchez López Jazmín Vanessa Pérez Pazos Hilda Adriana David Hinestroza Efecto de las PGPB sobre el crecimiento Pennisetum clandestinum bajo condiciones de estrés salino Revista Colombiana de Biotecnología Bacillus subtilis Bacillus amyloliquefaciens biofertilizantes salinidad solubilización de fósforo biofertilizer salinity phosphorus solubilization |
title | Efecto de las PGPB sobre el crecimiento Pennisetum clandestinum bajo condiciones de estrés salino |
title_full | Efecto de las PGPB sobre el crecimiento Pennisetum clandestinum bajo condiciones de estrés salino |
title_fullStr | Efecto de las PGPB sobre el crecimiento Pennisetum clandestinum bajo condiciones de estrés salino |
title_full_unstemmed | Efecto de las PGPB sobre el crecimiento Pennisetum clandestinum bajo condiciones de estrés salino |
title_short | Efecto de las PGPB sobre el crecimiento Pennisetum clandestinum bajo condiciones de estrés salino |
title_sort | efecto de las pgpb sobre el crecimiento pennisetum clandestinum bajo condiciones de estres salino |
topic | Bacillus subtilis Bacillus amyloliquefaciens biofertilizantes salinidad solubilización de fósforo biofertilizer salinity phosphorus solubilization |
url | http://www.revistas.unal.edu.co/index.php/biotecnologia/article/view/50413 |
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