Efecto de las PGPB sobre el crecimiento Pennisetum clandestinum bajo condiciones de estrés salino

This research aims the isolation and characterization of bacteria with potential to promote the growth of grass Pennisetum clandestinum in simulated saline soils. 92 Gram positive bacterias were isolated from Rhizosphere soil samples of P. clandestinum. Ten isolated bacteria grew on nutrient agar su...

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Main Authors: Diana Beatriz Sánchez López, Jazmín Vanessa Pérez Pazos, Hilda Adriana David Hinestroza
Format: Article
Language:Spanish
Published: Universidad Nacional de Colombia 2016-05-01
Series:Revista Colombiana de Biotecnología
Subjects:
Online Access:http://www.revistas.unal.edu.co/index.php/biotecnologia/article/view/50413
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author Diana Beatriz Sánchez López
Jazmín Vanessa Pérez Pazos
Hilda Adriana David Hinestroza
author_facet Diana Beatriz Sánchez López
Jazmín Vanessa Pérez Pazos
Hilda Adriana David Hinestroza
author_sort Diana Beatriz Sánchez López
collection DOAJ
description This research aims the isolation and characterization of bacteria with potential to promote the growth of grass Pennisetum clandestinum in simulated saline soils. 92 Gram positive bacterias were isolated from Rhizosphere soil samples of P. clandestinum. Ten isolated bacteria grew on nutrient agar supplemented with NaCl (2.578 M). Isolates were evaluated under greenhouse conditions; the strains identified as KISA 34 and KISA 71 were selected as the best based on the statistical test of Dunnet (p ≤ 0.05) and were identified Bacillus amyloliquefaciens KISA 34 and Bacillus subtilis KISA 71. These strains have the ability to produce ammonium, exopolysaccharides and cellulose, both in absence and presence of NaCl. The strains produced indoles and phosphorus solubilization. The evaluation of strains in greenhouse showed that the T6 - KISA 34 + KISA 71+ 25 % (phosphate rock RF) significantly increased biomass and plant development (p≤ 0.05) compared with T2 - full fertilization . The results of this research showed that isolates have the ability to grow in saline soils retaining its characteristics as promoting plant growth with positive effects on P. clandestinum.
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spelling doaj.art-47dc43675ca242d29ec737d015fb0ea62022-12-21T17:34:17ZspaUniversidad Nacional de ColombiaRevista Colombiana de Biotecnología0123-34751909-87582016-05-011816572Efecto de las PGPB sobre el crecimiento Pennisetum clandestinum bajo condiciones de estrés salinoDiana Beatriz Sánchez López0Jazmín Vanessa Pérez Pazos1Hilda Adriana David Hinestroza2M.Sc. Ciencias Biológicas. Centro de investigación Turipaná. Corporación Colombiana de Investigación Agropecuaria (Corpoica). km 13 vía Montería-Cereté, Colombia. Telephone +57(1) 4227300 Ext. 2249. E-mail correspondencia: dbsanchez@corpoica.org.coB.Sc Bióloga. Centro de investigación Turipaná. Corporación Colombiana de Investigación Agropecuaria (Corpoica). km 13 vía Montería-Cereté, Colombia. Telephone +57(1) 4227300 Ext. 2249. jvperez@corpoica.org.coM.Sc. Nutrición de Rumiantes. Centro de investigación Turipaná. Corporación Colombiana de Investigación Agropecuaria (Corpoica). km 13 vía Montería -Cereté, Colombia. adavid@corpoica.org.coThis research aims the isolation and characterization of bacteria with potential to promote the growth of grass Pennisetum clandestinum in simulated saline soils. 92 Gram positive bacterias were isolated from Rhizosphere soil samples of P. clandestinum. Ten isolated bacteria grew on nutrient agar supplemented with NaCl (2.578 M). Isolates were evaluated under greenhouse conditions; the strains identified as KISA 34 and KISA 71 were selected as the best based on the statistical test of Dunnet (p ≤ 0.05) and were identified Bacillus amyloliquefaciens KISA 34 and Bacillus subtilis KISA 71. These strains have the ability to produce ammonium, exopolysaccharides and cellulose, both in absence and presence of NaCl. The strains produced indoles and phosphorus solubilization. The evaluation of strains in greenhouse showed that the T6 - KISA 34 + KISA 71+ 25 % (phosphate rock RF) significantly increased biomass and plant development (p≤ 0.05) compared with T2 - full fertilization . The results of this research showed that isolates have the ability to grow in saline soils retaining its characteristics as promoting plant growth with positive effects on P. clandestinum.http://www.revistas.unal.edu.co/index.php/biotecnologia/article/view/50413Bacillus subtilisBacillus amyloliquefaciensbiofertilizantessalinidadsolubilización de fósforobiofertilizersalinityphosphorus solubilization
spellingShingle Diana Beatriz Sánchez López
Jazmín Vanessa Pérez Pazos
Hilda Adriana David Hinestroza
Efecto de las PGPB sobre el crecimiento Pennisetum clandestinum bajo condiciones de estrés salino
Revista Colombiana de Biotecnología
Bacillus subtilis
Bacillus amyloliquefaciens
biofertilizantes
salinidad
solubilización de fósforo
biofertilizer
salinity
phosphorus solubilization
title Efecto de las PGPB sobre el crecimiento Pennisetum clandestinum bajo condiciones de estrés salino
title_full Efecto de las PGPB sobre el crecimiento Pennisetum clandestinum bajo condiciones de estrés salino
title_fullStr Efecto de las PGPB sobre el crecimiento Pennisetum clandestinum bajo condiciones de estrés salino
title_full_unstemmed Efecto de las PGPB sobre el crecimiento Pennisetum clandestinum bajo condiciones de estrés salino
title_short Efecto de las PGPB sobre el crecimiento Pennisetum clandestinum bajo condiciones de estrés salino
title_sort efecto de las pgpb sobre el crecimiento pennisetum clandestinum bajo condiciones de estres salino
topic Bacillus subtilis
Bacillus amyloliquefaciens
biofertilizantes
salinidad
solubilización de fósforo
biofertilizer
salinity
phosphorus solubilization
url http://www.revistas.unal.edu.co/index.php/biotecnologia/article/view/50413
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