E-NPP3 controls plasmacytoid dendritic cell numbers in the small intestine.
Extracellular adenosine 5'-triphosphate (ATP) performs multiple functions including activation and induction of apoptosis of many cell types. The ATP-hydrolyzing ectoenzyme ecto-nucleotide pyrophosphatase/phosphodiesterase 3 (E-NPP3) regulates ATP-dependent chronic allergic responses by mast ce...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5321438?pdf=render |
| _version_ | 1818553679877242880 |
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| author | Yoki Furuta Shih-Han Tsai Makoto Kinoshita Kosuke Fujimoto Ryu Okumura Eiji Umemoto Yosuke Kurashima Hiroshi Kiyono Hisako Kayama Kiyoshi Takeda |
| author_facet | Yoki Furuta Shih-Han Tsai Makoto Kinoshita Kosuke Fujimoto Ryu Okumura Eiji Umemoto Yosuke Kurashima Hiroshi Kiyono Hisako Kayama Kiyoshi Takeda |
| author_sort | Yoki Furuta |
| collection | DOAJ |
| description | Extracellular adenosine 5'-triphosphate (ATP) performs multiple functions including activation and induction of apoptosis of many cell types. The ATP-hydrolyzing ectoenzyme ecto-nucleotide pyrophosphatase/phosphodiesterase 3 (E-NPP3) regulates ATP-dependent chronic allergic responses by mast cells and basophils. However, E-NPP3 is also highly expressed on epithelial cells of the small intestine. In this study, we showed that E-NPP3 controls plasmacytoid dendritic cell (pDC) numbers in the intestine through regulation of intestinal extracellular ATP. In Enpp3-/- mice, ATP concentrations were increased in the intestinal lumen. pDC numbers were remarkably decreased in the small intestinal lamina propria and Peyer's patches. Intestinal pDCs of Enpp3-/- mice showed enhanced cell death as characterized by increases in annexin V binding and expression of cleaved caspase-3. pDCs were highly sensitive to ATP-induced cell death compared with conventional DCs. ATP-induced cell death was abrogated in P2rx7-/- pDCs. Accordingly, the number of intestinal pDCs was restored in Enpp3-/- P2rx7-/- mice. These findings demonstrate that E-NPP3 regulates ATP concentration and thereby prevents the decrease of pDCs in the small intestine. |
| first_indexed | 2024-12-12T09:28:59Z |
| format | Article |
| id | doaj.art-47dd530950804d9591d889c31d2717dc |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T09:28:59Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-47dd530950804d9591d889c31d2717dc2022-12-22T00:28:56ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01122e017250910.1371/journal.pone.0172509E-NPP3 controls plasmacytoid dendritic cell numbers in the small intestine.Yoki FurutaShih-Han TsaiMakoto KinoshitaKosuke FujimotoRyu OkumuraEiji UmemotoYosuke KurashimaHiroshi KiyonoHisako KayamaKiyoshi TakedaExtracellular adenosine 5'-triphosphate (ATP) performs multiple functions including activation and induction of apoptosis of many cell types. The ATP-hydrolyzing ectoenzyme ecto-nucleotide pyrophosphatase/phosphodiesterase 3 (E-NPP3) regulates ATP-dependent chronic allergic responses by mast cells and basophils. However, E-NPP3 is also highly expressed on epithelial cells of the small intestine. In this study, we showed that E-NPP3 controls plasmacytoid dendritic cell (pDC) numbers in the intestine through regulation of intestinal extracellular ATP. In Enpp3-/- mice, ATP concentrations were increased in the intestinal lumen. pDC numbers were remarkably decreased in the small intestinal lamina propria and Peyer's patches. Intestinal pDCs of Enpp3-/- mice showed enhanced cell death as characterized by increases in annexin V binding and expression of cleaved caspase-3. pDCs were highly sensitive to ATP-induced cell death compared with conventional DCs. ATP-induced cell death was abrogated in P2rx7-/- pDCs. Accordingly, the number of intestinal pDCs was restored in Enpp3-/- P2rx7-/- mice. These findings demonstrate that E-NPP3 regulates ATP concentration and thereby prevents the decrease of pDCs in the small intestine.http://europepmc.org/articles/PMC5321438?pdf=render |
| spellingShingle | Yoki Furuta Shih-Han Tsai Makoto Kinoshita Kosuke Fujimoto Ryu Okumura Eiji Umemoto Yosuke Kurashima Hiroshi Kiyono Hisako Kayama Kiyoshi Takeda E-NPP3 controls plasmacytoid dendritic cell numbers in the small intestine. PLoS ONE |
| title | E-NPP3 controls plasmacytoid dendritic cell numbers in the small intestine. |
| title_full | E-NPP3 controls plasmacytoid dendritic cell numbers in the small intestine. |
| title_fullStr | E-NPP3 controls plasmacytoid dendritic cell numbers in the small intestine. |
| title_full_unstemmed | E-NPP3 controls plasmacytoid dendritic cell numbers in the small intestine. |
| title_short | E-NPP3 controls plasmacytoid dendritic cell numbers in the small intestine. |
| title_sort | e npp3 controls plasmacytoid dendritic cell numbers in the small intestine |
| url | http://europepmc.org/articles/PMC5321438?pdf=render |
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