Single-cell quantification and dose-response of cytosolic siRNA delivery
Endosomal escape and subsequent cytosolic delivery of siRNA therapeutics is inefficient, and quantification is difficult. Here the authors report a confocal microscopy-based method to quantify cytosolic delivery of fluorescently labelled siRNA during lipid-mediated delivery.
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-02-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-023-36752-1 |
| _version_ | 1827983532379602944 |
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| author | Hampus Hedlund Hampus Du Rietz Johanna M. Johansson Hanna C. Eriksson Wahed Zedan Linfeng Huang Jonas Wallin Anders Wittrup |
| author_facet | Hampus Hedlund Hampus Du Rietz Johanna M. Johansson Hanna C. Eriksson Wahed Zedan Linfeng Huang Jonas Wallin Anders Wittrup |
| author_sort | Hampus Hedlund |
| collection | DOAJ |
| description | Endosomal escape and subsequent cytosolic delivery of siRNA therapeutics is inefficient, and quantification is difficult. Here the authors report a confocal microscopy-based method to quantify cytosolic delivery of fluorescently labelled siRNA during lipid-mediated delivery. |
| first_indexed | 2024-04-09T22:49:12Z |
| format | Article |
| id | doaj.art-47df8d79ce954f14a790a888afd14d96 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-09T22:49:12Z |
| publishDate | 2023-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-47df8d79ce954f14a790a888afd14d962023-03-22T11:41:42ZengNature PortfolioNature Communications2041-17232023-02-0114111410.1038/s41467-023-36752-1Single-cell quantification and dose-response of cytosolic siRNA deliveryHampus Hedlund0Hampus Du Rietz1Johanna M. Johansson2Hanna C. Eriksson3Wahed Zedan4Linfeng Huang5Jonas Wallin6Anders Wittrup7Department of Clinical Sciences Lund, Oncology, Faculty of Medicine, Lund UniversityDepartment of Clinical Sciences Lund, Oncology, Faculty of Medicine, Lund UniversityDepartment of Clinical Sciences Lund, Oncology, Faculty of Medicine, Lund UniversityDepartment of Clinical Sciences Lund, Oncology, Faculty of Medicine, Lund UniversityDepartment of Clinical Sciences Lund, Oncology, Faculty of Medicine, Lund UniversityWang-Cai Biochemistry Lab, Division of Natural and Applied Sciences, Duke Kunshan UniversityDepartment of Mathematical Statistics, Lund UniversityDepartment of Clinical Sciences Lund, Oncology, Faculty of Medicine, Lund UniversityEndosomal escape and subsequent cytosolic delivery of siRNA therapeutics is inefficient, and quantification is difficult. Here the authors report a confocal microscopy-based method to quantify cytosolic delivery of fluorescently labelled siRNA during lipid-mediated delivery.https://doi.org/10.1038/s41467-023-36752-1 |
| spellingShingle | Hampus Hedlund Hampus Du Rietz Johanna M. Johansson Hanna C. Eriksson Wahed Zedan Linfeng Huang Jonas Wallin Anders Wittrup Single-cell quantification and dose-response of cytosolic siRNA delivery Nature Communications |
| title | Single-cell quantification and dose-response of cytosolic siRNA delivery |
| title_full | Single-cell quantification and dose-response of cytosolic siRNA delivery |
| title_fullStr | Single-cell quantification and dose-response of cytosolic siRNA delivery |
| title_full_unstemmed | Single-cell quantification and dose-response of cytosolic siRNA delivery |
| title_short | Single-cell quantification and dose-response of cytosolic siRNA delivery |
| title_sort | single cell quantification and dose response of cytosolic sirna delivery |
| url | https://doi.org/10.1038/s41467-023-36752-1 |
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