New Anti-RSV Nucleoprotein Monoclonal Antibody Pairs Discovered Using Rabbit Phage Display Technology
Human respiratory syncytial virus (hRSV) is one of the major contagious viruses and causes complicated respiratory issues, especially in young children. The sensitive and fast detection of hRSV is critical for taking the most effective actions. In the present study, rabbit antibodies against the hRS...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | Antibodies |
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| Online Access: | https://www.mdpi.com/2073-4468/12/4/73 |
| _version_ | 1827575821079937024 |
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| author | Pierre-Emmanuel Baurand Jérémy Balland Emilia Galli Suvi Eklin Rémy Bruley Laurence Ringenbach |
| author_facet | Pierre-Emmanuel Baurand Jérémy Balland Emilia Galli Suvi Eklin Rémy Bruley Laurence Ringenbach |
| author_sort | Pierre-Emmanuel Baurand |
| collection | DOAJ |
| description | Human respiratory syncytial virus (hRSV) is one of the major contagious viruses and causes complicated respiratory issues, especially in young children. The sensitive and fast detection of hRSV is critical for taking the most effective actions. In the present study, rabbit antibodies against the hRSV nucleoprotein (NP) were developed using phage display technology. A female rabbit was immunized with an hRSV strain A2 recombinant NP. A Fab library was built and sorted during two successive panning rounds for strain B and the A2 NP (recombinant preparations), respectively. The choice of candidates was performed using ELISA on the two NP strains. The obtained library was 3 × 10<sup>6</sup> cfu/mL, with an insertion rate of >95%. The two panning rounds permitted an enrichment factor of 100. ELISA screening allowed us to obtain 28 NP-specific Fab candidates. Among them, 10 retained candidates were reformatted into rabbit full IgG; thereafter, pairing tests on the recombinant strains and native lysate samples were performed. After the pairing tests on the recombinant strains, 53 pairs were identified. Eleven pairs were identified as being able to detect RSVs from native lysates. This work presents new high-potential monoclonal antibodies mAbs (mAbs), which would benefit from lateral flow testing data with patient materials. |
| first_indexed | 2024-03-08T21:02:24Z |
| format | Article |
| id | doaj.art-47dfc77d7bab4201b0b943d5177b48d7 |
| institution | Directory Open Access Journal |
| issn | 2073-4468 |
| language | English |
| last_indexed | 2024-03-08T21:02:24Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibodies |
| spelling | doaj.art-47dfc77d7bab4201b0b943d5177b48d72023-12-22T13:48:11ZengMDPI AGAntibodies2073-44682023-11-011247310.3390/antib12040073New Anti-RSV Nucleoprotein Monoclonal Antibody Pairs Discovered Using Rabbit Phage Display TechnologyPierre-Emmanuel Baurand0Jérémy Balland1Emilia Galli2Suvi Eklin3Rémy Bruley4Laurence Ringenbach5Diaclone SAS—Part of Medix Biochemica Group, 6 Rue Dr Jean-François-Xavier Girod, BP 1985, 25000 Besançon, FranceDiaclone SAS—Part of Medix Biochemica Group, 6 Rue Dr Jean-François-Xavier Girod, BP 1985, 25000 Besançon, FranceMedix Biochemica Group, Headquarter, Klovinpellontie 3, FI-02180 Espoo, FinlandMedix Biochemica Group, Headquarter, Klovinpellontie 3, FI-02180 Espoo, FinlandDiaclone SAS—Part of Medix Biochemica Group, 6 Rue Dr Jean-François-Xavier Girod, BP 1985, 25000 Besançon, FranceDiaclone SAS—Part of Medix Biochemica Group, 6 Rue Dr Jean-François-Xavier Girod, BP 1985, 25000 Besançon, FranceHuman respiratory syncytial virus (hRSV) is one of the major contagious viruses and causes complicated respiratory issues, especially in young children. The sensitive and fast detection of hRSV is critical for taking the most effective actions. In the present study, rabbit antibodies against the hRSV nucleoprotein (NP) were developed using phage display technology. A female rabbit was immunized with an hRSV strain A2 recombinant NP. A Fab library was built and sorted during two successive panning rounds for strain B and the A2 NP (recombinant preparations), respectively. The choice of candidates was performed using ELISA on the two NP strains. The obtained library was 3 × 10<sup>6</sup> cfu/mL, with an insertion rate of >95%. The two panning rounds permitted an enrichment factor of 100. ELISA screening allowed us to obtain 28 NP-specific Fab candidates. Among them, 10 retained candidates were reformatted into rabbit full IgG; thereafter, pairing tests on the recombinant strains and native lysate samples were performed. After the pairing tests on the recombinant strains, 53 pairs were identified. Eleven pairs were identified as being able to detect RSVs from native lysates. This work presents new high-potential monoclonal antibodies mAbs (mAbs), which would benefit from lateral flow testing data with patient materials.https://www.mdpi.com/2073-4468/12/4/73rabbitphage displayhuman respiratory syncytial virusmonoclonal antibodiesnucleoproteinELISA |
| spellingShingle | Pierre-Emmanuel Baurand Jérémy Balland Emilia Galli Suvi Eklin Rémy Bruley Laurence Ringenbach New Anti-RSV Nucleoprotein Monoclonal Antibody Pairs Discovered Using Rabbit Phage Display Technology Antibodies rabbit phage display human respiratory syncytial virus monoclonal antibodies nucleoprotein ELISA |
| title | New Anti-RSV Nucleoprotein Monoclonal Antibody Pairs Discovered Using Rabbit Phage Display Technology |
| title_full | New Anti-RSV Nucleoprotein Monoclonal Antibody Pairs Discovered Using Rabbit Phage Display Technology |
| title_fullStr | New Anti-RSV Nucleoprotein Monoclonal Antibody Pairs Discovered Using Rabbit Phage Display Technology |
| title_full_unstemmed | New Anti-RSV Nucleoprotein Monoclonal Antibody Pairs Discovered Using Rabbit Phage Display Technology |
| title_short | New Anti-RSV Nucleoprotein Monoclonal Antibody Pairs Discovered Using Rabbit Phage Display Technology |
| title_sort | new anti rsv nucleoprotein monoclonal antibody pairs discovered using rabbit phage display technology |
| topic | rabbit phage display human respiratory syncytial virus monoclonal antibodies nucleoprotein ELISA |
| url | https://www.mdpi.com/2073-4468/12/4/73 |
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