Plasma metabolomics profiles in Black and White participants of the Adventist Health Study-2 cohort
Abstract Background Black Americans suffer disparities in risk for cardiometabolic and other chronic diseases. Findings from the Adventist Health Study-2 (AHS-2) cohort have shown associations of plant-based dietary patterns and healthy lifestyle factors with prevention of such diseases. Hence, it i...
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| Format: | Article |
| Language: | English |
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BMC
2023-10-01
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| Series: | BMC Medicine |
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| Online Access: | https://doi.org/10.1186/s12916-023-03101-4 |
| _version_ | 1797636811985518592 |
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| author | Fayth M. Butler Jason Utt Roy O. Mathew Carlos A. Casiano Suzanne Montgomery Seth A. Wiafe Johanna W. Lampe Gary E. Fraser |
| author_facet | Fayth M. Butler Jason Utt Roy O. Mathew Carlos A. Casiano Suzanne Montgomery Seth A. Wiafe Johanna W. Lampe Gary E. Fraser |
| author_sort | Fayth M. Butler |
| collection | DOAJ |
| description | Abstract Background Black Americans suffer disparities in risk for cardiometabolic and other chronic diseases. Findings from the Adventist Health Study-2 (AHS-2) cohort have shown associations of plant-based dietary patterns and healthy lifestyle factors with prevention of such diseases. Hence, it is likely that racial differences in metabolic profiles correlating with disparities in chronic diseases are explained largely by diet and lifestyle, besides social determinants of health. Methods Untargeted plasma metabolomics screening was performed on plasma samples from 350 participants of the AHS-2, including 171 Black and 179 White participants, using ultrahigh-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) and a global platform of 892 metabolites. Differences in metabolites or biochemical subclasses by race were analyzed using linear regression, considering various models adjusted for known confounders, dietary and/or other lifestyle behaviors, social vulnerability, and psychosocial stress. The Storey permutation approach was used to adjust for false discovery at FDR < 0.05. Results Linear regression revealed differential abundance of over 40% of individual metabolites or biochemical subclasses when comparing Black with White participants after adjustment for false discovery (FDR < 0.05), with the vast majority showing lower abundance in Blacks. Associations were not appreciably altered with adjustment for dietary patterns and socioeconomic or psychosocial stress. Metabolite subclasses showing consistently lower abundance in Black participants included various lipids, such as lysophospholipids, phosphatidylethanolamines, monoacylglycerols, diacylglycerols, and long-chain monounsaturated fatty acids, among other subclasses or lipid categories. Among all biochemical subclasses, creatine metabolism exclusively showed higher abundance in Black participants, although among metabolites within this subclass, only creatine showed differential abundance after adjustment for glomerular filtration rate. Notable metabolites in higher abundance in Black participants included methyl and propyl paraben sulfates, piperine metabolites, and a considerable proportion of acetylated amino acids, including many previously found associated with glomerular filtration rate. Conclusions Differences in metabolic profiles were evident when comparing Black and White participants of the AHS-2 cohort. These differences are likely attributed in part to dietary behaviors not adequately explained by dietary pattern covariates, besides other environmental or genetic factors. Alterations in these metabolites and associated subclasses may have implications for the prevention of chronic diseases in Black Americans. |
| first_indexed | 2024-03-11T12:40:29Z |
| format | Article |
| id | doaj.art-47e16b7eba28444195a9f2aa5a3f2c55 |
| institution | Directory Open Access Journal |
| issn | 1741-7015 |
| language | English |
| last_indexed | 2024-03-11T12:40:29Z |
| publishDate | 2023-10-01 |
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| spelling | doaj.art-47e16b7eba28444195a9f2aa5a3f2c552023-11-05T12:20:52ZengBMCBMC Medicine1741-70152023-10-0121111710.1186/s12916-023-03101-4Plasma metabolomics profiles in Black and White participants of the Adventist Health Study-2 cohortFayth M. Butler0Jason Utt1Roy O. Mathew2Carlos A. Casiano3Suzanne Montgomery4Seth A. Wiafe5Johanna W. Lampe6Gary E. Fraser7Adventist Health Study, Loma Linda UniversityAdventist Health Study, Loma Linda UniversityDivision of Nephrology, Department of Medicine, Loma Linda VA Health Care SystemCenter for Health Disparities and Molecular Medicine, Loma Linda University School of MedicineCenter for Health Disparities and Molecular Medicine, Loma Linda University School of MedicineCenter for Leadership in Health Systems, School of Public Health, Loma Linda UniversityPublic Health Sciences Division, Fred Hutchinson Cancer CenterAdventist Health Study, Loma Linda UniversityAbstract Background Black Americans suffer disparities in risk for cardiometabolic and other chronic diseases. Findings from the Adventist Health Study-2 (AHS-2) cohort have shown associations of plant-based dietary patterns and healthy lifestyle factors with prevention of such diseases. Hence, it is likely that racial differences in metabolic profiles correlating with disparities in chronic diseases are explained largely by diet and lifestyle, besides social determinants of health. Methods Untargeted plasma metabolomics screening was performed on plasma samples from 350 participants of the AHS-2, including 171 Black and 179 White participants, using ultrahigh-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) and a global platform of 892 metabolites. Differences in metabolites or biochemical subclasses by race were analyzed using linear regression, considering various models adjusted for known confounders, dietary and/or other lifestyle behaviors, social vulnerability, and psychosocial stress. The Storey permutation approach was used to adjust for false discovery at FDR < 0.05. Results Linear regression revealed differential abundance of over 40% of individual metabolites or biochemical subclasses when comparing Black with White participants after adjustment for false discovery (FDR < 0.05), with the vast majority showing lower abundance in Blacks. Associations were not appreciably altered with adjustment for dietary patterns and socioeconomic or psychosocial stress. Metabolite subclasses showing consistently lower abundance in Black participants included various lipids, such as lysophospholipids, phosphatidylethanolamines, monoacylglycerols, diacylglycerols, and long-chain monounsaturated fatty acids, among other subclasses or lipid categories. Among all biochemical subclasses, creatine metabolism exclusively showed higher abundance in Black participants, although among metabolites within this subclass, only creatine showed differential abundance after adjustment for glomerular filtration rate. Notable metabolites in higher abundance in Black participants included methyl and propyl paraben sulfates, piperine metabolites, and a considerable proportion of acetylated amino acids, including many previously found associated with glomerular filtration rate. Conclusions Differences in metabolic profiles were evident when comparing Black and White participants of the AHS-2 cohort. These differences are likely attributed in part to dietary behaviors not adequately explained by dietary pattern covariates, besides other environmental or genetic factors. Alterations in these metabolites and associated subclasses may have implications for the prevention of chronic diseases in Black Americans.https://doi.org/10.1186/s12916-023-03101-4MetabolomicsCohortDietary patternHealth disparitiesBlack AmericansLinear regression |
| spellingShingle | Fayth M. Butler Jason Utt Roy O. Mathew Carlos A. Casiano Suzanne Montgomery Seth A. Wiafe Johanna W. Lampe Gary E. Fraser Plasma metabolomics profiles in Black and White participants of the Adventist Health Study-2 cohort BMC Medicine Metabolomics Cohort Dietary pattern Health disparities Black Americans Linear regression |
| title | Plasma metabolomics profiles in Black and White participants of the Adventist Health Study-2 cohort |
| title_full | Plasma metabolomics profiles in Black and White participants of the Adventist Health Study-2 cohort |
| title_fullStr | Plasma metabolomics profiles in Black and White participants of the Adventist Health Study-2 cohort |
| title_full_unstemmed | Plasma metabolomics profiles in Black and White participants of the Adventist Health Study-2 cohort |
| title_short | Plasma metabolomics profiles in Black and White participants of the Adventist Health Study-2 cohort |
| title_sort | plasma metabolomics profiles in black and white participants of the adventist health study 2 cohort |
| topic | Metabolomics Cohort Dietary pattern Health disparities Black Americans Linear regression |
| url | https://doi.org/10.1186/s12916-023-03101-4 |
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