<i>GNB3</i> c.825C>T (rs5443) Polymorphism and Risk of Acute Cardiovascular Events after Renal Allograft Transplant
The c.825C>T single-nucleotide polymorphism (rs5443) of the guanine nucleotide-binding protein subunit β3 (<i>GNB3</i>) results in increased intracellular signal transduction via G-proteins. The present study investigated the effect of the <i>GNB3</i> c.825C>T polymorph...
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MDPI AG
2022-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/17/9783 |
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| author | Tobias Peitz Birte Möhlendick Winfried Siffert Falko Markus Heinemann Andreas Kribben Ute Eisenberger Justa Friebus-Kardash |
| author_facet | Tobias Peitz Birte Möhlendick Winfried Siffert Falko Markus Heinemann Andreas Kribben Ute Eisenberger Justa Friebus-Kardash |
| author_sort | Tobias Peitz |
| collection | DOAJ |
| description | The c.825C>T single-nucleotide polymorphism (rs5443) of the guanine nucleotide-binding protein subunit β3 (<i>GNB3</i>) results in increased intracellular signal transduction via G-proteins. The present study investigated the effect of the <i>GNB3</i> c.825C>T polymorphism on cardiovascular events among renal allograft recipients posttransplant. Our retrospective study involved 436 renal allograft recipients who were followed up for up to 8 years after transplant. The <i>GNB3</i> c.825C>T polymorphism was detected with restriction fragment length polymorphism (RFLP) polymerase chain reaction (PCR). The <i>GNB3</i> TT genotype was detected in 43 (10%) of 436 recipients. Death due to an acute cardiovascular event occurred more frequently among recipients with the TT genotype (4 [9%]) than among those with the CC/CT genotypes (7 [2%]; <i>p</i> = 0.003). The rates of myocardial infarction (MI)–free survival (<i>p</i> = 0.003) and acute peripheral artery occlusive disease (PAOD)–free survival (<i>p</i> = 0.004) were significantly lower among T-homozygous patients. A multivariate analysis showed that homozygous <i>GNB3</i> c.825C>T polymorphism exerted only a mild effect for the occurrence of myocardial infarction (relative risk, 2.2; <i>p</i> = 0.065) or acute PAOD (relative risk, 2.4; <i>p</i> = 0.05) after renal transplant. Our results suggest that the homozygous <i>GNB3</i> T allele exerts noticeable effects on the risk of MI and acute PAOD only in the presence of additional nonheritable risk factors. |
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| language | English |
| last_indexed | 2024-03-10T01:45:05Z |
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| spelling | doaj.art-47e342a2ca234be1b810c9c85f91775f2023-11-23T13:16:55ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-08-012317978310.3390/ijms23179783<i>GNB3</i> c.825C>T (rs5443) Polymorphism and Risk of Acute Cardiovascular Events after Renal Allograft TransplantTobias Peitz0Birte Möhlendick1Winfried Siffert2Falko Markus Heinemann3Andreas Kribben4Ute Eisenberger5Justa Friebus-Kardash6Department of Nephrology, University Hospital Essen, University of Duisburg-Essen, Hufelandstr. 55, 45147 Essen, GermanyInstitute of Pharmacogenetics, University Hospital Essen, University of Duisburg-Essen, 45147 Essen, GermanyInstitute of Pharmacogenetics, University Hospital Essen, University of Duisburg-Essen, 45147 Essen, GermanyInstitute for Transfusion Medicine, Transplantation Diagnostics, University Hospital Essen, University of Duisburg-Essen, 45147 Essen, GermanyDepartment of Nephrology, University Hospital Essen, University of Duisburg-Essen, Hufelandstr. 55, 45147 Essen, GermanyDepartment of Nephrology, University Hospital Essen, University of Duisburg-Essen, Hufelandstr. 55, 45147 Essen, GermanyDepartment of Nephrology, University Hospital Essen, University of Duisburg-Essen, Hufelandstr. 55, 45147 Essen, GermanyThe c.825C>T single-nucleotide polymorphism (rs5443) of the guanine nucleotide-binding protein subunit β3 (<i>GNB3</i>) results in increased intracellular signal transduction via G-proteins. The present study investigated the effect of the <i>GNB3</i> c.825C>T polymorphism on cardiovascular events among renal allograft recipients posttransplant. Our retrospective study involved 436 renal allograft recipients who were followed up for up to 8 years after transplant. The <i>GNB3</i> c.825C>T polymorphism was detected with restriction fragment length polymorphism (RFLP) polymerase chain reaction (PCR). The <i>GNB3</i> TT genotype was detected in 43 (10%) of 436 recipients. Death due to an acute cardiovascular event occurred more frequently among recipients with the TT genotype (4 [9%]) than among those with the CC/CT genotypes (7 [2%]; <i>p</i> = 0.003). The rates of myocardial infarction (MI)–free survival (<i>p</i> = 0.003) and acute peripheral artery occlusive disease (PAOD)–free survival (<i>p</i> = 0.004) were significantly lower among T-homozygous patients. A multivariate analysis showed that homozygous <i>GNB3</i> c.825C>T polymorphism exerted only a mild effect for the occurrence of myocardial infarction (relative risk, 2.2; <i>p</i> = 0.065) or acute PAOD (relative risk, 2.4; <i>p</i> = 0.05) after renal transplant. Our results suggest that the homozygous <i>GNB3</i> T allele exerts noticeable effects on the risk of MI and acute PAOD only in the presence of additional nonheritable risk factors.https://www.mdpi.com/1422-0067/23/17/9783<i>GNB3</i> c.825C>T polymorphismrenal transplantationmyocardial infarctionacute peripheral artery occlusive disease |
| spellingShingle | Tobias Peitz Birte Möhlendick Winfried Siffert Falko Markus Heinemann Andreas Kribben Ute Eisenberger Justa Friebus-Kardash <i>GNB3</i> c.825C>T (rs5443) Polymorphism and Risk of Acute Cardiovascular Events after Renal Allograft Transplant International Journal of Molecular Sciences <i>GNB3</i> c.825C>T polymorphism renal transplantation myocardial infarction acute peripheral artery occlusive disease |
| title | <i>GNB3</i> c.825C>T (rs5443) Polymorphism and Risk of Acute Cardiovascular Events after Renal Allograft Transplant |
| title_full | <i>GNB3</i> c.825C>T (rs5443) Polymorphism and Risk of Acute Cardiovascular Events after Renal Allograft Transplant |
| title_fullStr | <i>GNB3</i> c.825C>T (rs5443) Polymorphism and Risk of Acute Cardiovascular Events after Renal Allograft Transplant |
| title_full_unstemmed | <i>GNB3</i> c.825C>T (rs5443) Polymorphism and Risk of Acute Cardiovascular Events after Renal Allograft Transplant |
| title_short | <i>GNB3</i> c.825C>T (rs5443) Polymorphism and Risk of Acute Cardiovascular Events after Renal Allograft Transplant |
| title_sort | i gnb3 i c 825c t rs5443 polymorphism and risk of acute cardiovascular events after renal allograft transplant |
| topic | <i>GNB3</i> c.825C>T polymorphism renal transplantation myocardial infarction acute peripheral artery occlusive disease |
| url | https://www.mdpi.com/1422-0067/23/17/9783 |
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