IRF4-activated TEX41 promotes the malignant behaviors of melanoma cells by targeting miR-103a-3p/C1QB axis
Abstract Background Malignant melanoma is an aggressive skin cancer and a tumor of melanocytic origin. Recent studies have suggested that long non-coding RNAs (lncRNAs) play crucial regulatory roles in multiple malignancies, including melanoma. Testis expressed 41 (TEX41) is a relatively new lncRNA...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-12-01
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| Series: | BMC Cancer |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12885-021-09039-1 |
| _version_ | 1818873500605087744 |
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| author | Yingna Zheng Wu Zhou Min Li Ruixue Xu Shuai Zhang Ying Liu Ying Cen |
| author_facet | Yingna Zheng Wu Zhou Min Li Ruixue Xu Shuai Zhang Ying Liu Ying Cen |
| author_sort | Yingna Zheng |
| collection | DOAJ |
| description | Abstract Background Malignant melanoma is an aggressive skin cancer and a tumor of melanocytic origin. Recent studies have suggested that long non-coding RNAs (lncRNAs) play crucial regulatory roles in multiple malignancies, including melanoma. Testis expressed 41 (TEX41) is a relatively new lncRNA whose mechanism in melanoma remains vague. Aims This study aimed to explore the role and specific mechanism of TEX41 in melanoma. Methods The expression of genes involved in this study was determined by qRT-PCR. Functional assays were conducted to analyze the role of relevant genes in melanoma cells. The interaction between TEX41 promoter and IRF4 as well as the relationship among TEX41, miR-103a-3p and C1QB was verified by mechanism assays. Results IRF4 up-regulated TEX41 at the transcriptional level in melanoma cells. TEX41 knockdown hindered melanoma cell proliferation, migration and invasion while promoting cell apoptosis. TEX41 bound to miR-103a-3p and regulated C1QB. The suppressive impact of TEX41 depletion on melanoma cell malignant behaviors could be counteracted by miR-103a-3p inhibition or C1QB overexpression. Moreover, IRF4 could facilitate melanoma cell growth via up-regulating C1QB. Conclusions IRF4-activated TEX41 sequestered miR-103a-3p and modulated C1QB to promote melanoma cell malignant behaviors, for which TEX41 might be regarded as a potential therapeutic target for melanoma. |
| first_indexed | 2024-12-19T12:55:42Z |
| format | Article |
| id | doaj.art-47f93f0c54b540bf80ac9b12c5d1ecb7 |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-12-19T12:55:42Z |
| publishDate | 2021-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-47f93f0c54b540bf80ac9b12c5d1ecb72022-12-21T20:20:24ZengBMCBMC Cancer1471-24072021-12-0121111410.1186/s12885-021-09039-1IRF4-activated TEX41 promotes the malignant behaviors of melanoma cells by targeting miR-103a-3p/C1QB axisYingna Zheng0Wu Zhou1Min Li2Ruixue Xu3Shuai Zhang4Ying Liu5Ying Cen6Department of Dermatology, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, People’s Hospital of Henan UniversityDepartment of Dermatology, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, People’s Hospital of Henan UniversityDepartment of Dermatology, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, People’s Hospital of Henan UniversityDepartment of Dermatology, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, People’s Hospital of Henan UniversityDepartment of Dermatology, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, People’s Hospital of Henan UniversityDepartment of Dermatology, Xianyang Central HospitalDepartment of Plastic and Burn Surgery, West China Hospital, West China School of Medicine, Sichuan UniversityAbstract Background Malignant melanoma is an aggressive skin cancer and a tumor of melanocytic origin. Recent studies have suggested that long non-coding RNAs (lncRNAs) play crucial regulatory roles in multiple malignancies, including melanoma. Testis expressed 41 (TEX41) is a relatively new lncRNA whose mechanism in melanoma remains vague. Aims This study aimed to explore the role and specific mechanism of TEX41 in melanoma. Methods The expression of genes involved in this study was determined by qRT-PCR. Functional assays were conducted to analyze the role of relevant genes in melanoma cells. The interaction between TEX41 promoter and IRF4 as well as the relationship among TEX41, miR-103a-3p and C1QB was verified by mechanism assays. Results IRF4 up-regulated TEX41 at the transcriptional level in melanoma cells. TEX41 knockdown hindered melanoma cell proliferation, migration and invasion while promoting cell apoptosis. TEX41 bound to miR-103a-3p and regulated C1QB. The suppressive impact of TEX41 depletion on melanoma cell malignant behaviors could be counteracted by miR-103a-3p inhibition or C1QB overexpression. Moreover, IRF4 could facilitate melanoma cell growth via up-regulating C1QB. Conclusions IRF4-activated TEX41 sequestered miR-103a-3p and modulated C1QB to promote melanoma cell malignant behaviors, for which TEX41 might be regarded as a potential therapeutic target for melanoma.https://doi.org/10.1186/s12885-021-09039-1MelanomaTEX41IRF4miR-103a-3pC1QB |
| spellingShingle | Yingna Zheng Wu Zhou Min Li Ruixue Xu Shuai Zhang Ying Liu Ying Cen IRF4-activated TEX41 promotes the malignant behaviors of melanoma cells by targeting miR-103a-3p/C1QB axis BMC Cancer Melanoma TEX41 IRF4 miR-103a-3p C1QB |
| title | IRF4-activated TEX41 promotes the malignant behaviors of melanoma cells by targeting miR-103a-3p/C1QB axis |
| title_full | IRF4-activated TEX41 promotes the malignant behaviors of melanoma cells by targeting miR-103a-3p/C1QB axis |
| title_fullStr | IRF4-activated TEX41 promotes the malignant behaviors of melanoma cells by targeting miR-103a-3p/C1QB axis |
| title_full_unstemmed | IRF4-activated TEX41 promotes the malignant behaviors of melanoma cells by targeting miR-103a-3p/C1QB axis |
| title_short | IRF4-activated TEX41 promotes the malignant behaviors of melanoma cells by targeting miR-103a-3p/C1QB axis |
| title_sort | irf4 activated tex41 promotes the malignant behaviors of melanoma cells by targeting mir 103a 3p c1qb axis |
| topic | Melanoma TEX41 IRF4 miR-103a-3p C1QB |
| url | https://doi.org/10.1186/s12885-021-09039-1 |
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