Detection of viral infection by immunofluorescence in formalin-fixed tissues, pretreated with trypsin
The presence of viral antigen in sections from formalin-fixed and paraffin-embedded human tissues was demonstrated by trypsin digestion followed by direct or indirect immunofluorescence. The specimens may be used for retrospective diagnosis. The immunofluorescence technique has to be adapted to the...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
1988-06-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761988000200010 |
| _version_ | 1797704790225977344 |
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| author | O. M. Barth S. Majerowicz L. P. Menasce H. G. Schatzmayr |
| author_facet | O. M. Barth S. Majerowicz L. P. Menasce H. G. Schatzmayr |
| author_sort | O. M. Barth |
| collection | DOAJ |
| description | The presence of viral antigen in sections from formalin-fixed and paraffin-embedded human tissues was demonstrated by trypsin digestion followed by direct or indirect immunofluorescence. The specimens may be used for retrospective diagnosis. The immunofluorescence technique has to be adapted to the suspected virus infection on the basis of previous histopathology study. Variations of trypsin concentration time and temperature of incubation, expose different viral antigens and have to be previously tested for each unknown system. For measles virus detection in lung a stronger digestion has to be applied as compared to adenovirus or respiratory disease viruses in the same tisue. Flavivirus in liver tissue needs a weaker digestion. The reproducibility of the method makes it useful as a routine technique in diagnosis of virus infection.<br>A presença de antígeno viral em cortes de tecidos humanos fixados em formol e emblocados em parafina foi demonstrada pela digestão com tripsina foi demonstrada pela ingestão com tripsina seguida de imunofluorescência direta ou indireta. Os espécimens podem ser utilizados para diagnoses retrospectivas. A técnica da imunofluorescência deve ser adaptada à infecção viral suspeita segundo diagnosie histopatológica prévia. Os parâmetros para a digestão do tecido pela tripsina, relacionados à concentração, duração de atuação e temperatura, expõem diferentes antígenos virais e devem ser previamente testados para cada sistema a ser estabelecido. Uma digestão mais intensa deve ser aplicada para a detecção do vírus do sarampo em tecido pulmonar do que para adenovírus ou vírus respiratório sincicial no mesmo tecido. Por outro lado, o vírus da febre amarela em tecido de fígado necessita de uma digestão mais fraca. |
| first_indexed | 2024-03-12T05:25:41Z |
| format | Article |
| id | doaj.art-47fadc001116486b997d0991fa07db25 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T05:25:41Z |
| publishDate | 1988-06-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-47fadc001116486b997d0991fa07db252023-09-03T07:24:29ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80601988-06-0183220721210.1590/S0074-02761988000200010Detection of viral infection by immunofluorescence in formalin-fixed tissues, pretreated with trypsinO. M. BarthS. MajerowiczL. P. MenasceH. G. SchatzmayrThe presence of viral antigen in sections from formalin-fixed and paraffin-embedded human tissues was demonstrated by trypsin digestion followed by direct or indirect immunofluorescence. The specimens may be used for retrospective diagnosis. The immunofluorescence technique has to be adapted to the suspected virus infection on the basis of previous histopathology study. Variations of trypsin concentration time and temperature of incubation, expose different viral antigens and have to be previously tested for each unknown system. For measles virus detection in lung a stronger digestion has to be applied as compared to adenovirus or respiratory disease viruses in the same tisue. Flavivirus in liver tissue needs a weaker digestion. The reproducibility of the method makes it useful as a routine technique in diagnosis of virus infection.<br>A presença de antígeno viral em cortes de tecidos humanos fixados em formol e emblocados em parafina foi demonstrada pela digestão com tripsina foi demonstrada pela ingestão com tripsina seguida de imunofluorescência direta ou indireta. Os espécimens podem ser utilizados para diagnoses retrospectivas. A técnica da imunofluorescência deve ser adaptada à infecção viral suspeita segundo diagnosie histopatológica prévia. Os parâmetros para a digestão do tecido pela tripsina, relacionados à concentração, duração de atuação e temperatura, expõem diferentes antígenos virais e devem ser previamente testados para cada sistema a ser estabelecido. Uma digestão mais intensa deve ser aplicada para a detecção do vírus do sarampo em tecido pulmonar do que para adenovírus ou vírus respiratório sincicial no mesmo tecido. Por outro lado, o vírus da febre amarela em tecido de fígado necessita de uma digestão mais fraca.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761988000200010diagnose viralimmunofluorescênciadiagnose retrospectivadigestão por tripsinavirus diagnosisimmunofluorescenceretrospective diagnosistrypsin digestion |
| spellingShingle | O. M. Barth S. Majerowicz L. P. Menasce H. G. Schatzmayr Detection of viral infection by immunofluorescence in formalin-fixed tissues, pretreated with trypsin Memorias do Instituto Oswaldo Cruz diagnose viral immunofluorescência diagnose retrospectiva digestão por tripsina virus diagnosis immunofluorescence retrospective diagnosis trypsin digestion |
| title | Detection of viral infection by immunofluorescence in formalin-fixed tissues, pretreated with trypsin |
| title_full | Detection of viral infection by immunofluorescence in formalin-fixed tissues, pretreated with trypsin |
| title_fullStr | Detection of viral infection by immunofluorescence in formalin-fixed tissues, pretreated with trypsin |
| title_full_unstemmed | Detection of viral infection by immunofluorescence in formalin-fixed tissues, pretreated with trypsin |
| title_short | Detection of viral infection by immunofluorescence in formalin-fixed tissues, pretreated with trypsin |
| title_sort | detection of viral infection by immunofluorescence in formalin fixed tissues pretreated with trypsin |
| topic | diagnose viral immunofluorescência diagnose retrospectiva digestão por tripsina virus diagnosis immunofluorescence retrospective diagnosis trypsin digestion |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761988000200010 |
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