Differential protein analysis of saline-alkali promoting the oil accumulation in Nitzschia palea
Abstract Background The increasingly severe salinization of the aquatic environment has led to serious damage to the habitats of aquatic organisms. Benthic diatoms are commonly employed as indicator species for assessing water quality and serve as a reflection of the overall health of the aquatic ec...
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| Format: | Article |
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BMC
2024-01-01
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| Series: | Biotechnology for Biofuels and Bioproducts |
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| Online Access: | https://doi.org/10.1186/s13068-023-02451-8 |
| _version_ | 1797275475197820928 |
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| author | Xintong Wang Xianghong Meng Yanlong Dong Chunhua Song Fengyang Sui Xinxin Lu Xiaoxue Mei Yawen Fan Yan Liu |
| author_facet | Xintong Wang Xianghong Meng Yanlong Dong Chunhua Song Fengyang Sui Xinxin Lu Xiaoxue Mei Yawen Fan Yan Liu |
| author_sort | Xintong Wang |
| collection | DOAJ |
| description | Abstract Background The increasingly severe salinization of the aquatic environment has led to serious damage to the habitats of aquatic organisms. Benthic diatoms are commonly employed as indicator species for assessing water quality and serve as a reflection of the overall health of the aquatic ecosystem. Nitzschia palea is a common diatom found in freshwater, with high oil content, rapid reproductive rate, and it is a commonly dominant species in various rivers. Results The results showed that after 4 days (d) of saline-alkali stress, the cell density and chlorophyll a content of Nitzschia palea reached their maximum values. Therefore, we selected Nitzschia palea under 4 d stress for Tandem Mass Tag (TMT) quantitative proteomic analysis to explore the molecular adaptation mechanism of freshwater diatoms under saline-alkali stress. Totally, 854 proteins were enriched, of which 439 differentially expressed proteins were identified. Gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and subcellular fractionation analysis revealed that these proteins were mainly enriched in the photosynthesis pathway, citric acid cycle (TCA cycle), fatty acid synthesis, and glutathione cycle. Conclusions This study aims to reveal the physiological, biochemical and proteomic mechanisms of salt and alkali tolerance and molecular adaptation of Nitzschia palea under different saline-alkali concentrations. This study showed that Nitzschia palea is one candidate of the environmental friendly, renewable bioenergy microalgae. Meantime, Nitzschia palea reveals for the proteome of the freshwater and provides the basis, it became a model algal species for freshwater diatoms. |
| first_indexed | 2024-03-07T15:14:54Z |
| format | Article |
| id | doaj.art-48031ed97a50439cbcb79448f340c39c |
| institution | Directory Open Access Journal |
| issn | 2731-3654 |
| language | English |
| last_indexed | 2024-03-07T15:14:54Z |
| publishDate | 2024-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels and Bioproducts |
| spelling | doaj.art-48031ed97a50439cbcb79448f340c39c2024-03-05T17:57:49ZengBMCBiotechnology for Biofuels and Bioproducts2731-36542024-01-0117111710.1186/s13068-023-02451-8Differential protein analysis of saline-alkali promoting the oil accumulation in Nitzschia paleaXintong Wang0Xianghong Meng1Yanlong Dong2Chunhua Song3Fengyang Sui4Xinxin Lu5Xiaoxue Mei6Yawen Fan7Yan Liu8College of Life Sciences and Technology, Harbin Normal UniversityCollege of Life Sciences and Technology, Harbin Normal UniversityCollege of Life Sciences and Technology, Harbin Normal UniversityCollege of Life Sciences and Technology, Harbin Normal UniversityCollege of Life Sciences and Technology, Harbin Normal UniversityCollege of Life Sciences and Technology, Harbin Normal UniversityCollege of Life Sciences and Technology, Harbin Normal UniversityCollege of Life Sciences and Technology, Harbin Normal UniversityCollege of Life Sciences and Technology, Harbin Normal UniversityAbstract Background The increasingly severe salinization of the aquatic environment has led to serious damage to the habitats of aquatic organisms. Benthic diatoms are commonly employed as indicator species for assessing water quality and serve as a reflection of the overall health of the aquatic ecosystem. Nitzschia palea is a common diatom found in freshwater, with high oil content, rapid reproductive rate, and it is a commonly dominant species in various rivers. Results The results showed that after 4 days (d) of saline-alkali stress, the cell density and chlorophyll a content of Nitzschia palea reached their maximum values. Therefore, we selected Nitzschia palea under 4 d stress for Tandem Mass Tag (TMT) quantitative proteomic analysis to explore the molecular adaptation mechanism of freshwater diatoms under saline-alkali stress. Totally, 854 proteins were enriched, of which 439 differentially expressed proteins were identified. Gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and subcellular fractionation analysis revealed that these proteins were mainly enriched in the photosynthesis pathway, citric acid cycle (TCA cycle), fatty acid synthesis, and glutathione cycle. Conclusions This study aims to reveal the physiological, biochemical and proteomic mechanisms of salt and alkali tolerance and molecular adaptation of Nitzschia palea under different saline-alkali concentrations. This study showed that Nitzschia palea is one candidate of the environmental friendly, renewable bioenergy microalgae. Meantime, Nitzschia palea reveals for the proteome of the freshwater and provides the basis, it became a model algal species for freshwater diatoms.https://doi.org/10.1186/s13068-023-02451-8Saline-alkaliNitzschia paleaProteomicsAlgae oilFatty acidsFreshwater diatoms |
| spellingShingle | Xintong Wang Xianghong Meng Yanlong Dong Chunhua Song Fengyang Sui Xinxin Lu Xiaoxue Mei Yawen Fan Yan Liu Differential protein analysis of saline-alkali promoting the oil accumulation in Nitzschia palea Biotechnology for Biofuels and Bioproducts Saline-alkali Nitzschia palea Proteomics Algae oil Fatty acids Freshwater diatoms |
| title | Differential protein analysis of saline-alkali promoting the oil accumulation in Nitzschia palea |
| title_full | Differential protein analysis of saline-alkali promoting the oil accumulation in Nitzschia palea |
| title_fullStr | Differential protein analysis of saline-alkali promoting the oil accumulation in Nitzschia palea |
| title_full_unstemmed | Differential protein analysis of saline-alkali promoting the oil accumulation in Nitzschia palea |
| title_short | Differential protein analysis of saline-alkali promoting the oil accumulation in Nitzschia palea |
| title_sort | differential protein analysis of saline alkali promoting the oil accumulation in nitzschia palea |
| topic | Saline-alkali Nitzschia palea Proteomics Algae oil Fatty acids Freshwater diatoms |
| url | https://doi.org/10.1186/s13068-023-02451-8 |
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