Genome-wide analysis of the AP2/ERF gene family in Tritipyrum and the response of TtERF_B2-50 in salt-tolerance
Abstract The AP2/ERF transcription factor is widely distributed across the plant kingdom and plays a crucial role in various abiotic stress responses in plants. Tritipyrum, an octoploid resulting from an intergeneric cross between Triticum aestivum (AABBDD) and Thinopyrum elongatum (EE), is a valuab...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-09-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-023-09585-x |
| _version_ | 1797453803527602176 |
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| author | Xiaojuan Liu Guangyi Zhou Songshu Chen Zhenzhen Jia Suqin Zhang Mingjian Ren Fang He |
| author_facet | Xiaojuan Liu Guangyi Zhou Songshu Chen Zhenzhen Jia Suqin Zhang Mingjian Ren Fang He |
| author_sort | Xiaojuan Liu |
| collection | DOAJ |
| description | Abstract The AP2/ERF transcription factor is widely distributed across the plant kingdom and plays a crucial role in various abiotic stress responses in plants. Tritipyrum, an octoploid resulting from an intergeneric cross between Triticum aestivum (AABBDD) and Thinopyrum elongatum (EE), is a valuable source of germplasm for incorporating superior traits of Th. elongatum into T. aestivum. With the recent availability of whole -genome sequences for T. aestivum and Th. elongatum, we explored the organization and expression profiling of Tritipyrum AP2/ERF genes across the entire genome. Our investigation identified 543 Tritipyrum AP2/ERF genes, which evolutionary analysis categorized into four major groups (AP2, DREB, ERF, and RAV), whose members share a conserved motif composition. These 543 TtAP2/ERF genes were distributed throughout 28 chromosomes, with 132 duplications. Synteny analysis suggests that the AP2/ERF gene family may have a common ancestor. Transcriptome data and Real-Time PCR expression profiles revealed 43 TtAP2/ERF genes with high expression levels in response to various salt stressors and recovery regimens. Tel2E01T236300 (TtERF_B2-50) was particularly salt stress-sensitive and evolutionarily related to the salt-tolerant gene AtERF7 in A. thaliana. Pearson correlation analysis identified 689 genes positively correlated (R > 0.9) with TtERF_B2-50 expression, enriched in metabolic activities, cellular processes, stimulus response, and biological regulation. Real-time PCR showed that TtERF_B2-50 was highly expressed in roots, stems, and leaves under salt stress. These findings suggest that TtERF_B2-50 may be associated with salt stress tolerance and may serve as a valuable foreign gene for enhancing salt tolerance in wheat. |
| first_indexed | 2024-03-09T15:28:09Z |
| format | Article |
| id | doaj.art-48059f0d1a1747dead07219ca207b2e4 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-03-09T15:28:09Z |
| publishDate | 2023-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-48059f0d1a1747dead07219ca207b2e42023-11-26T12:25:09ZengBMCBMC Genomics1471-21642023-09-0124111510.1186/s12864-023-09585-xGenome-wide analysis of the AP2/ERF gene family in Tritipyrum and the response of TtERF_B2-50 in salt-toleranceXiaojuan Liu0Guangyi Zhou1Songshu Chen2Zhenzhen Jia3Suqin Zhang4Mingjian Ren5Fang He6Guizhou Subcenter of National Wheat Improvement Center, Agronomy College, Guizhou UniversityGuizhou Subcenter of National Wheat Improvement Center, Agronomy College, Guizhou UniversityGuizhou Subcenter of National Wheat Improvement Center, Agronomy College, Guizhou UniversitySchool of Life Sciences, Guizhou Normal UniversityGuizhou Subcenter of National Wheat Improvement Center, Agronomy College, Guizhou UniversityGuizhou Subcenter of National Wheat Improvement Center, Agronomy College, Guizhou UniversityGuizhou Subcenter of National Wheat Improvement Center, Agronomy College, Guizhou UniversityAbstract The AP2/ERF transcription factor is widely distributed across the plant kingdom and plays a crucial role in various abiotic stress responses in plants. Tritipyrum, an octoploid resulting from an intergeneric cross between Triticum aestivum (AABBDD) and Thinopyrum elongatum (EE), is a valuable source of germplasm for incorporating superior traits of Th. elongatum into T. aestivum. With the recent availability of whole -genome sequences for T. aestivum and Th. elongatum, we explored the organization and expression profiling of Tritipyrum AP2/ERF genes across the entire genome. Our investigation identified 543 Tritipyrum AP2/ERF genes, which evolutionary analysis categorized into four major groups (AP2, DREB, ERF, and RAV), whose members share a conserved motif composition. These 543 TtAP2/ERF genes were distributed throughout 28 chromosomes, with 132 duplications. Synteny analysis suggests that the AP2/ERF gene family may have a common ancestor. Transcriptome data and Real-Time PCR expression profiles revealed 43 TtAP2/ERF genes with high expression levels in response to various salt stressors and recovery regimens. Tel2E01T236300 (TtERF_B2-50) was particularly salt stress-sensitive and evolutionarily related to the salt-tolerant gene AtERF7 in A. thaliana. Pearson correlation analysis identified 689 genes positively correlated (R > 0.9) with TtERF_B2-50 expression, enriched in metabolic activities, cellular processes, stimulus response, and biological regulation. Real-time PCR showed that TtERF_B2-50 was highly expressed in roots, stems, and leaves under salt stress. These findings suggest that TtERF_B2-50 may be associated with salt stress tolerance and may serve as a valuable foreign gene for enhancing salt tolerance in wheat.https://doi.org/10.1186/s12864-023-09585-xTritipyrumAP2/ERFSalt-toleranceTtERF_B2-50Genome-wideExpression patterns |
| spellingShingle | Xiaojuan Liu Guangyi Zhou Songshu Chen Zhenzhen Jia Suqin Zhang Mingjian Ren Fang He Genome-wide analysis of the AP2/ERF gene family in Tritipyrum and the response of TtERF_B2-50 in salt-tolerance BMC Genomics Tritipyrum AP2/ERF Salt-tolerance TtERF_B2-50 Genome-wide Expression patterns |
| title | Genome-wide analysis of the AP2/ERF gene family in Tritipyrum and the response of TtERF_B2-50 in salt-tolerance |
| title_full | Genome-wide analysis of the AP2/ERF gene family in Tritipyrum and the response of TtERF_B2-50 in salt-tolerance |
| title_fullStr | Genome-wide analysis of the AP2/ERF gene family in Tritipyrum and the response of TtERF_B2-50 in salt-tolerance |
| title_full_unstemmed | Genome-wide analysis of the AP2/ERF gene family in Tritipyrum and the response of TtERF_B2-50 in salt-tolerance |
| title_short | Genome-wide analysis of the AP2/ERF gene family in Tritipyrum and the response of TtERF_B2-50 in salt-tolerance |
| title_sort | genome wide analysis of the ap2 erf gene family in tritipyrum and the response of tterf b2 50 in salt tolerance |
| topic | Tritipyrum AP2/ERF Salt-tolerance TtERF_B2-50 Genome-wide Expression patterns |
| url | https://doi.org/10.1186/s12864-023-09585-x |
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