Determination of the presence of 5-methylcytosine in Paramecium tetraurelia.
5-methylcytosine DNA methylation regulates gene expression and developmental programming in a broad range of eukaryotes. However, its presence and potential roles in ciliates, complex single-celled eukaryotes with germline-somatic genome specialization via nuclear dimorphism, are largely uncharted....
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2018-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC6209305?pdf=render |
| _version_ | 1818449791577751552 |
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| author | Aditi Singh Adrienne Vancura Rafal K Woycicki Daniel J Hogan Alan G Hendrick Mariusz Nowacki |
| author_facet | Aditi Singh Adrienne Vancura Rafal K Woycicki Daniel J Hogan Alan G Hendrick Mariusz Nowacki |
| author_sort | Aditi Singh |
| collection | DOAJ |
| description | 5-methylcytosine DNA methylation regulates gene expression and developmental programming in a broad range of eukaryotes. However, its presence and potential roles in ciliates, complex single-celled eukaryotes with germline-somatic genome specialization via nuclear dimorphism, are largely uncharted. While canonical cytosine methyltransferases have not been discovered in published ciliate genomes, recent studies performed in the stichotrichous ciliate Oxytricha trifallax suggest de novo cytosine methylation during macronuclear development. In this study, we applied bisulfite genome sequencing, DNA mass spectrometry and antibody-based fluorescence detection to investigate the presence of DNA methylation in Paramecium tetraurelia. While the antibody-based methods suggest cytosine methylation, DNA mass spectrometry and bisulfite sequencing reveal that levels are actually below the limit of detection. Our results suggest that Paramecium does not utilize 5-methylcytosine DNA methylation as an integral part of its epigenetic arsenal. |
| first_indexed | 2024-12-14T20:41:01Z |
| format | Article |
| id | doaj.art-4805bb1580ff418c9f9c1a793f850174 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T20:41:01Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-4805bb1580ff418c9f9c1a793f8501742022-12-21T22:48:16ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-011310e020666710.1371/journal.pone.0206667Determination of the presence of 5-methylcytosine in Paramecium tetraurelia.Aditi SinghAdrienne VancuraRafal K WoycickiDaniel J HoganAlan G HendrickMariusz Nowacki5-methylcytosine DNA methylation regulates gene expression and developmental programming in a broad range of eukaryotes. However, its presence and potential roles in ciliates, complex single-celled eukaryotes with germline-somatic genome specialization via nuclear dimorphism, are largely uncharted. While canonical cytosine methyltransferases have not been discovered in published ciliate genomes, recent studies performed in the stichotrichous ciliate Oxytricha trifallax suggest de novo cytosine methylation during macronuclear development. In this study, we applied bisulfite genome sequencing, DNA mass spectrometry and antibody-based fluorescence detection to investigate the presence of DNA methylation in Paramecium tetraurelia. While the antibody-based methods suggest cytosine methylation, DNA mass spectrometry and bisulfite sequencing reveal that levels are actually below the limit of detection. Our results suggest that Paramecium does not utilize 5-methylcytosine DNA methylation as an integral part of its epigenetic arsenal.http://europepmc.org/articles/PMC6209305?pdf=render |
| spellingShingle | Aditi Singh Adrienne Vancura Rafal K Woycicki Daniel J Hogan Alan G Hendrick Mariusz Nowacki Determination of the presence of 5-methylcytosine in Paramecium tetraurelia. PLoS ONE |
| title | Determination of the presence of 5-methylcytosine in Paramecium tetraurelia. |
| title_full | Determination of the presence of 5-methylcytosine in Paramecium tetraurelia. |
| title_fullStr | Determination of the presence of 5-methylcytosine in Paramecium tetraurelia. |
| title_full_unstemmed | Determination of the presence of 5-methylcytosine in Paramecium tetraurelia. |
| title_short | Determination of the presence of 5-methylcytosine in Paramecium tetraurelia. |
| title_sort | determination of the presence of 5 methylcytosine in paramecium tetraurelia |
| url | http://europepmc.org/articles/PMC6209305?pdf=render |
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