Anti-Diabetic Potential of Ocimum gratissimum Leaf Fractions in Fortified Diet-Fed Streptozotocin Treated Rat Model of Type-2 Diabetes
Background: Ocimum gratissimum (OG) is used in the traditional management of diabetes in Nigeria. This study investigated the anti-diabetic potential of OG leaf fractions (OGLF) in a rat model of Type-2 diabetes (T2D). Method: Methanol crude extract of OG leaf was fractionated with solvents of incre...
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MDPI AG
2017-10-01
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| Series: | Medicines |
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| Online Access: | https://www.mdpi.com/2305-6320/4/4/73 |
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| author | Stanley I. R. Okoduwa Isamila A. Umar Dorcas B. James Hajiya M. Inuwa |
| author_facet | Stanley I. R. Okoduwa Isamila A. Umar Dorcas B. James Hajiya M. Inuwa |
| author_sort | Stanley I. R. Okoduwa |
| collection | DOAJ |
| description | Background: Ocimum gratissimum (OG) is used in the traditional management of diabetes in Nigeria. This study investigated the anti-diabetic potential of OG leaf fractions (OGLF) in a rat model of Type-2 diabetes (T2D). Method: Methanol crude extract of OG leaf was fractionated with solvents of increasing order of polarity (n-hexane, chloroform, ethyl-acetate, n-butanol and water). The anti-diabetic potential of the fractions was evaluated in vivo. T2D was induced in Albino Wistar rats and treated with OGLF. Result: The T2D rats showed significant elevation in serum levels of fasting blood glucose (FBG), liver and kidney function biomarkers. At 4-weeks of intervention with OGLF, the untreated diabetic control group maintained severe hyperglycaemia in the presence of 61.7% serum insulin, 17.3% pancreatic β-cell function (HOMA-β) and 51.5% Insulin sensitivity. The glucose tolerance ability was enhanced in the n-butanol-fraction (OGb) treated group. With 74.8% available serum insulin and 38.6% improvement in insulin sensitivity, the OGb treated group had a 63.5% reduction in FBG and it was found to be most effective as it ameliorates a majority of the changes caused in the studied parameters in diabetic rats. Conclusions: The data from this study suggest that OGb fraction is a potential candidate for the development of an effective drug for the management of T2D. |
| first_indexed | 2024-12-10T18:55:59Z |
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| id | doaj.art-48070ca0c4e54c3e97d01d25c79dafc2 |
| institution | Directory Open Access Journal |
| issn | 2305-6320 |
| language | English |
| last_indexed | 2024-12-10T18:55:59Z |
| publishDate | 2017-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Medicines |
| spelling | doaj.art-48070ca0c4e54c3e97d01d25c79dafc22022-12-22T01:37:09ZengMDPI AGMedicines2305-63202017-10-01447310.3390/medicines4040073medicines4040073Anti-Diabetic Potential of Ocimum gratissimum Leaf Fractions in Fortified Diet-Fed Streptozotocin Treated Rat Model of Type-2 DiabetesStanley I. R. Okoduwa0Isamila A. Umar1Dorcas B. James2Hajiya M. Inuwa3Department of Biochemistry, Ahmadu Bello University, Zaria 811104, NigeriaDepartment of Biochemistry, Ahmadu Bello University, Zaria 811104, NigeriaDepartment of Biochemistry, Ahmadu Bello University, Zaria 811104, NigeriaDepartment of Biochemistry, Ahmadu Bello University, Zaria 811104, NigeriaBackground: Ocimum gratissimum (OG) is used in the traditional management of diabetes in Nigeria. This study investigated the anti-diabetic potential of OG leaf fractions (OGLF) in a rat model of Type-2 diabetes (T2D). Method: Methanol crude extract of OG leaf was fractionated with solvents of increasing order of polarity (n-hexane, chloroform, ethyl-acetate, n-butanol and water). The anti-diabetic potential of the fractions was evaluated in vivo. T2D was induced in Albino Wistar rats and treated with OGLF. Result: The T2D rats showed significant elevation in serum levels of fasting blood glucose (FBG), liver and kidney function biomarkers. At 4-weeks of intervention with OGLF, the untreated diabetic control group maintained severe hyperglycaemia in the presence of 61.7% serum insulin, 17.3% pancreatic β-cell function (HOMA-β) and 51.5% Insulin sensitivity. The glucose tolerance ability was enhanced in the n-butanol-fraction (OGb) treated group. With 74.8% available serum insulin and 38.6% improvement in insulin sensitivity, the OGb treated group had a 63.5% reduction in FBG and it was found to be most effective as it ameliorates a majority of the changes caused in the studied parameters in diabetic rats. Conclusions: The data from this study suggest that OGb fraction is a potential candidate for the development of an effective drug for the management of T2D.https://www.mdpi.com/2305-6320/4/4/73Type-2 diabetesOcimum gratissimumhyperglycaemiahyperlipidaemiarats |
| spellingShingle | Stanley I. R. Okoduwa Isamila A. Umar Dorcas B. James Hajiya M. Inuwa Anti-Diabetic Potential of Ocimum gratissimum Leaf Fractions in Fortified Diet-Fed Streptozotocin Treated Rat Model of Type-2 Diabetes Medicines Type-2 diabetes Ocimum gratissimum hyperglycaemia hyperlipidaemia rats |
| title | Anti-Diabetic Potential of Ocimum gratissimum Leaf Fractions in Fortified Diet-Fed Streptozotocin Treated Rat Model of Type-2 Diabetes |
| title_full | Anti-Diabetic Potential of Ocimum gratissimum Leaf Fractions in Fortified Diet-Fed Streptozotocin Treated Rat Model of Type-2 Diabetes |
| title_fullStr | Anti-Diabetic Potential of Ocimum gratissimum Leaf Fractions in Fortified Diet-Fed Streptozotocin Treated Rat Model of Type-2 Diabetes |
| title_full_unstemmed | Anti-Diabetic Potential of Ocimum gratissimum Leaf Fractions in Fortified Diet-Fed Streptozotocin Treated Rat Model of Type-2 Diabetes |
| title_short | Anti-Diabetic Potential of Ocimum gratissimum Leaf Fractions in Fortified Diet-Fed Streptozotocin Treated Rat Model of Type-2 Diabetes |
| title_sort | anti diabetic potential of ocimum gratissimum leaf fractions in fortified diet fed streptozotocin treated rat model of type 2 diabetes |
| topic | Type-2 diabetes Ocimum gratissimum hyperglycaemia hyperlipidaemia rats |
| url | https://www.mdpi.com/2305-6320/4/4/73 |
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