Detection of Clostridium perfringens types through genetic profiling and mouse neutralisation test
The aim of this study was to detect the types of C. perfringens using PCR and mouse neutralisation test (MNT) and find the genetic profile of virulence factors tpeL and netB isolated from animal samples in Iran. For this purpose, 46 C. perfringens isolates were tested using MNT. Detection was also p...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria
2023-09-01
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| Series: | Bulgarian Journal of Veterinary Medicine |
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| _version_ | 1797783996877242368 |
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| author | L. Abdolmohammadi Khiav A. Zahmatkesh A. Paradise |
| author_facet | L. Abdolmohammadi Khiav A. Zahmatkesh A. Paradise |
| author_sort | L. Abdolmohammadi Khiav |
| collection | DOAJ |
| description | The aim of this study was to detect the types of C. perfringens using PCR and mouse neutralisation test (MNT) and find the genetic profile of virulence factors tpeL and netB isolated from animal samples in Iran. For this purpose, 46 C. perfringens isolates were tested using MNT. Detection was also performed using 16S rRNA-PCR and multiplex PCR for alpha, beta, epsilon and iota toxins. For tpeL and netB gene profiling, separate PCR experiments were carried out. MNT detected that all isolates were C. perfringens type A. The same results were obtained in 16SrRNA-PCR and multiplex-PCR. Based on the obtained results, the molecular test was 100% consistent with MNT. Samples were positive for tpeL and netB genes in 52.1% and 60.8% isolates, respectively. Overall, four genetic virulence factor profiles were found in C. perfringens type A isolates. |
| first_indexed | 2024-03-13T00:33:43Z |
| format | Article |
| id | doaj.art-4808e08c700e40b589fbf6f700cc4d17 |
| institution | Directory Open Access Journal |
| issn | 1311-1477 1313-3543 |
| language | English |
| last_indexed | 2024-03-13T00:33:43Z |
| publishDate | 2023-09-01 |
| publisher | Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria |
| record_format | Article |
| series | Bulgarian Journal of Veterinary Medicine |
| spelling | doaj.art-4808e08c700e40b589fbf6f700cc4d172023-07-10T07:06:07ZengFaculty of Veterinary Medicine, Trakia University, Stara Zagora, BulgariaBulgarian Journal of Veterinary Medicine1311-14771313-35432023-09-0126338138910.15547/bjvm.2021-0060Detection of Clostridium perfringens types through genetic profiling and mouse neutralisation testL. Abdolmohammadi Khiav0A. Zahmatkesh 1A. Paradise2Department of Anaerobic Vaccine Research and Production, Specialized Clostridia Research Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, IranDepartment of Anaerobic Vaccine Research and Production, Specialized Clostridia Research Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, IranDepartment of Anaerobic Vaccine Research and Production, Specialized Clostridia Research Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, IranThe aim of this study was to detect the types of C. perfringens using PCR and mouse neutralisation test (MNT) and find the genetic profile of virulence factors tpeL and netB isolated from animal samples in Iran. For this purpose, 46 C. perfringens isolates were tested using MNT. Detection was also performed using 16S rRNA-PCR and multiplex PCR for alpha, beta, epsilon and iota toxins. For tpeL and netB gene profiling, separate PCR experiments were carried out. MNT detected that all isolates were C. perfringens type A. The same results were obtained in 16SrRNA-PCR and multiplex-PCR. Based on the obtained results, the molecular test was 100% consistent with MNT. Samples were positive for tpeL and netB genes in 52.1% and 60.8% isolates, respectively. Overall, four genetic virulence factor profiles were found in C. perfringens type A isolates. alpha toxinclostridium perfringensnetbpcrtpel |
| spellingShingle | L. Abdolmohammadi Khiav A. Zahmatkesh A. Paradise Detection of Clostridium perfringens types through genetic profiling and mouse neutralisation test Bulgarian Journal of Veterinary Medicine alpha toxin clostridium perfringens netb pcr tpel |
| title | Detection of Clostridium perfringens types through genetic profiling and mouse neutralisation test |
| title_full | Detection of Clostridium perfringens types through genetic profiling and mouse neutralisation test |
| title_fullStr | Detection of Clostridium perfringens types through genetic profiling and mouse neutralisation test |
| title_full_unstemmed | Detection of Clostridium perfringens types through genetic profiling and mouse neutralisation test |
| title_short | Detection of Clostridium perfringens types through genetic profiling and mouse neutralisation test |
| title_sort | detection of clostridium perfringens types through genetic profiling and mouse neutralisation test |
| topic | alpha toxin clostridium perfringens netb pcr tpel |
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