Development of Domestic Cat Embryo Produced by Preserved Sperms
The ability to mature and fertilize oocytes of endangered species may allow us to sustain genetic and global biodiversity. Epididymis sperms may be the last chance to ensure preservation of genetic materials after injury or death of a valuable animal. Studies have been conducted to determine wether...
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| Format: | Article |
| Language: | English |
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Bogor Agricultural University
2008-12-01
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| Series: | Hayati Journal of Biosciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1978301916302856 |
| _version_ | 1828778077960798208 |
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| author | KARTINI ERIANI ARIEF BOEDIONO ITA DJUWITA SONY HERU SUMARSONO AL-AZHAR |
| author_facet | KARTINI ERIANI ARIEF BOEDIONO ITA DJUWITA SONY HERU SUMARSONO AL-AZHAR |
| author_sort | KARTINI ERIANI |
| collection | DOAJ |
| description | The ability to mature and fertilize oocytes of endangered species may allow us to sustain genetic and global biodiversity. Epididymis sperms may be the last chance to ensure preservation of genetic materials after injury or death of a valuable animal. Studies have been conducted to determine wether both epididymis sperms and oocytes can be used to produce viable embryos and offspring. The purpose of this study was to determine how long cats sperms contained in epididymis were remain motile and had intact membranes when preserved at 4 ° C, and to determine whether such those preserved sperms are able to fertilize oocytes. Epididymis was preserved immediately in phosphate buffer saline at 4 ° C for 1, 3, and 6 days. The observation of sperm quality and viability after preservation was performed by vital staining acrosom and Hoechst-Propidium Iodine. Biological functions of sperms were evaluated by in vitro culture technique for fertilization, micro fertilization and embryonic development rate in CR1aa medium. The results showed that average motility of sperms collected from ductus deferens, cauda and corpus epididymis decreased not significantly (P > 0.05) from 0, 1, 3, and 6 days of preservation times (from 83.0%, 80.2%, 79.0%; 80.9%, 75.0%, 75.5%; 52.0%, 63.2%, 55.0% to 34.6%, 34.6%, 33.3%, respectively). The general results showed that sperms from epididymis preserved for 1, 3, and 6 days can be used for IVF. The rate of embryonal cleavage produced by IVF technique using sperms collected from epididymis preserved for 1-, 3- and 6-days were 33.3, 26.7, and 20.0%, respectively and significantly different (p < 0.05) from that of controll (50.0%). In conclusion, sperms contained in epididyimis preserved at 4 ° C in PBS (Phospate Buffer Saline) for 1-6 days can be used to IVF and in vitro production of cat embryos. |
| first_indexed | 2024-12-11T16:34:02Z |
| format | Article |
| id | doaj.art-4808f943e166422f802e81ebabfe562b |
| institution | Directory Open Access Journal |
| issn | 1978-3019 |
| language | English |
| last_indexed | 2024-12-11T16:34:02Z |
| publishDate | 2008-12-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Hayati Journal of Biosciences |
| spelling | doaj.art-4808f943e166422f802e81ebabfe562b2022-12-22T00:58:30ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192008-12-0115415516010.4308/hjb.15.4.155Development of Domestic Cat Embryo Produced by Preserved SpermsKARTINI ERIANI0ARIEF BOEDIONO1ITA DJUWITA2SONY HERU SUMARSONO3AL-AZHAR4Faculty of Mathematics and Science, Syiah Kuala University, Jalan Syeh Abdurrauf 3, Banda Aceh 23111, IndonesiaFaculty of Veterinary Medicine, Bogor Agricultural University, Jalan Rasamala, Darmaga Campus, Bogor 16680, IndonesiaFaculty of Veterinary Medicine, Bogor Agricultural University, Jalan Rasamala, Darmaga Campus, Bogor 16680, IndonesiaFaculty of Mathematics and Science, Institut Teknologi Bandung, Jalan Ganesha 10, Bandung 40132, IndonesiaFaculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh 23111, IndonesiaThe ability to mature and fertilize oocytes of endangered species may allow us to sustain genetic and global biodiversity. Epididymis sperms may be the last chance to ensure preservation of genetic materials after injury or death of a valuable animal. Studies have been conducted to determine wether both epididymis sperms and oocytes can be used to produce viable embryos and offspring. The purpose of this study was to determine how long cats sperms contained in epididymis were remain motile and had intact membranes when preserved at 4 ° C, and to determine whether such those preserved sperms are able to fertilize oocytes. Epididymis was preserved immediately in phosphate buffer saline at 4 ° C for 1, 3, and 6 days. The observation of sperm quality and viability after preservation was performed by vital staining acrosom and Hoechst-Propidium Iodine. Biological functions of sperms were evaluated by in vitro culture technique for fertilization, micro fertilization and embryonic development rate in CR1aa medium. The results showed that average motility of sperms collected from ductus deferens, cauda and corpus epididymis decreased not significantly (P > 0.05) from 0, 1, 3, and 6 days of preservation times (from 83.0%, 80.2%, 79.0%; 80.9%, 75.0%, 75.5%; 52.0%, 63.2%, 55.0% to 34.6%, 34.6%, 33.3%, respectively). The general results showed that sperms from epididymis preserved for 1, 3, and 6 days can be used for IVF. The rate of embryonal cleavage produced by IVF technique using sperms collected from epididymis preserved for 1-, 3- and 6-days were 33.3, 26.7, and 20.0%, respectively and significantly different (p < 0.05) from that of controll (50.0%). In conclusion, sperms contained in epididyimis preserved at 4 ° C in PBS (Phospate Buffer Saline) for 1-6 days can be used to IVF and in vitro production of cat embryos.http://www.sciencedirect.com/science/article/pii/S1978301916302856gametpreservationin vitro fertilization |
| spellingShingle | KARTINI ERIANI ARIEF BOEDIONO ITA DJUWITA SONY HERU SUMARSONO AL-AZHAR Development of Domestic Cat Embryo Produced by Preserved Sperms Hayati Journal of Biosciences gamet preservation in vitro fertilization |
| title | Development of Domestic Cat Embryo Produced by Preserved Sperms |
| title_full | Development of Domestic Cat Embryo Produced by Preserved Sperms |
| title_fullStr | Development of Domestic Cat Embryo Produced by Preserved Sperms |
| title_full_unstemmed | Development of Domestic Cat Embryo Produced by Preserved Sperms |
| title_short | Development of Domestic Cat Embryo Produced by Preserved Sperms |
| title_sort | development of domestic cat embryo produced by preserved sperms |
| topic | gamet preservation in vitro fertilization |
| url | http://www.sciencedirect.com/science/article/pii/S1978301916302856 |
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