Toxicity Going Nano: Ionic Versus Engineered Cu Nanoparticles Impacts on the Physiological Fitness of the Model Diatom Phaeodactylum tricornutum
Increasing input of Metal Engineered Nano Particles (MeENPs) in marine ecosystems has raised concerns about their potential toxicity on phytoplankton. Given the lack of knowledge on MeENPs impact on these important primary producers, the effects of Copper Oxide (CuO) ENPs on growth, physiology, pigm...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-12-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2020.539827/full |
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| author | Marco Franzitta Marco Franzitta Eduardo Feijão Eduardo Feijão Maria Teresa Cabrita Carla Gameiro Carla Gameiro Ana Rita Matos Ana Rita Matos João Carlos Marques Johannes W. Goessling Patrick Reis-Santos Patrick Reis-Santos Vanessa F. Fonseca Vanessa F. Fonseca Carlo Pretti Isabel Caçador Isabel Caçador Bernardo Duarte Bernardo Duarte |
| author_facet | Marco Franzitta Marco Franzitta Eduardo Feijão Eduardo Feijão Maria Teresa Cabrita Carla Gameiro Carla Gameiro Ana Rita Matos Ana Rita Matos João Carlos Marques Johannes W. Goessling Patrick Reis-Santos Patrick Reis-Santos Vanessa F. Fonseca Vanessa F. Fonseca Carlo Pretti Isabel Caçador Isabel Caçador Bernardo Duarte Bernardo Duarte |
| author_sort | Marco Franzitta |
| collection | DOAJ |
| description | Increasing input of Metal Engineered Nano Particles (MeENPs) in marine ecosystems has raised concerns about their potential toxicity on phytoplankton. Given the lack of knowledge on MeENPs impact on these important primary producers, the effects of Copper Oxide (CuO) ENPs on growth, physiology, pigment profiles, fatty acid (FA) metabolism, and oxidative stress were investigated in the model diatom Pheodactylum tricornutum, to provide suitable biomarkers of CuO ENP exposure versus its ionic counterpart. Diatom growth was inhibited by CuO ENPs but not Ionic Cu, suggesting CuO ENP cytotoxicity. Pulse Modulated Amplitude (PAM) phenotyping evidenced a decrease in the electron transport energy flux, pointing to a reduction in chemical energy generation following CuO ENPs exposure, as well as an increase in the content of the non-functional Cu-substituted chlorophyll a (CuChl a). A significant decrease in eicosapentaenoic acid (C20:5) associated with a significant rise in thylakoid membranes FAs reflected the activation of counteractive measures to photosynthetic impairment. Significant increase in the omega 6/omega 3 ratio, underline expectable negative repercussions to marine food webs. Increased thiobarbituric acid reactive substances reflected heightened oxidative stress by CuO ENP. Enhanced Glutathione Reductase and Ascorbate Peroxidase activity were also more evident for CuO ENPs than ionic Cu. Overall, observed molecular changes highlighted a battery of possible suitable biomarkers to efficiently determine the harmful effects of CuO ENPs. The results suggest that the occurrence and contamination of these new forms of metal contaminants can impose added stress to the marine diatom community, which could have significant impacts on marine ecosystems, namely through a reduction of the primary productivity, oxygen production and omega 6 production, all essential to sustain heterotrophic marine life. |
| first_indexed | 2024-12-14T02:21:21Z |
| format | Article |
| id | doaj.art-480ca5e0097647df874aef4acff6487c |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-12-14T02:21:21Z |
| publishDate | 2020-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-480ca5e0097647df874aef4acff6487c2022-12-21T23:20:30ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452020-12-01710.3389/fmars.2020.539827539827Toxicity Going Nano: Ionic Versus Engineered Cu Nanoparticles Impacts on the Physiological Fitness of the Model Diatom Phaeodactylum tricornutumMarco Franzitta0Marco Franzitta1Eduardo Feijão2Eduardo Feijão3Maria Teresa Cabrita4Carla Gameiro5Carla Gameiro6Ana Rita Matos7Ana Rita Matos8João Carlos Marques9Johannes W. Goessling10Patrick Reis-Santos11Patrick Reis-Santos12Vanessa F. Fonseca13Vanessa F. Fonseca14Carlo Pretti15Isabel Caçador16Isabel Caçador17Bernardo Duarte18Bernardo Duarte19MARE – Marine and Environmental Sciences Centre, Faculdade de Ciências da Universidade de Lisboa, Lisbon, PortugalDepartment of Veterinary Sciences, University of Pisa, Pisa, ItalyMARE – Marine and Environmental Sciences Centre, Faculdade de Ciências da Universidade de Lisboa, Lisbon, PortugalBioISI – Biosystems and Integrative Sciences Institute, Plant Functional Genomics Group, Departamento de Biologia Vegetal, Faculdade de Ciências da Universidade de Lisboa, Lisbon, PortugalInstituto do Mar e da Atmosfera, Lisbon, PortugalMARE – Marine and Environmental Sciences Centre, Faculdade de Ciências da Universidade de Lisboa, Lisbon, PortugalInstituto do Mar e da Atmosfera, Lisbon, PortugalBioISI – Biosystems and Integrative Sciences Institute, Plant Functional Genomics Group, Departamento de Biologia Vegetal, Faculdade de Ciências da Universidade de Lisboa, Lisbon, PortugalDepartamento de Biologia Vegetal, Faculdade de Ciências da Universidade de Lisboa, Lisbon, PortugalMARE – Marine and Environmental Sciences Centre, c/o Department of Zoology, Faculty of Sciences and Technology, University of Coimbra, Coimbra, PortugalInternational Iberian Nanotechnology Laboratory, Braga, PortugalMARE – Marine and Environmental Sciences Centre, Faculdade de Ciências da Universidade de Lisboa, Lisbon, PortugalSouthern Seas Ecology Laboratories, School of Biological Sciences, The University of Adelaide, Adelaide, SA, AustraliaMARE – Marine and Environmental Sciences Centre, Faculdade de Ciências da Universidade de Lisboa, Lisbon, PortugalDepartamento de Biologia Animal, Faculdade de Ciências da Universidade de Lisboa, Lisbon, PortugalDepartment of Veterinary Sciences, University of Pisa, Pisa, ItalyMARE – Marine and Environmental Sciences Centre, Faculdade de Ciências da Universidade de Lisboa, Lisbon, PortugalDepartamento de Biologia Vegetal, Faculdade de Ciências da Universidade de Lisboa, Lisbon, PortugalMARE – Marine and Environmental Sciences Centre, Faculdade de Ciências da Universidade de Lisboa, Lisbon, PortugalDepartamento de Biologia Vegetal, Faculdade de Ciências da Universidade de Lisboa, Lisbon, PortugalIncreasing input of Metal Engineered Nano Particles (MeENPs) in marine ecosystems has raised concerns about their potential toxicity on phytoplankton. Given the lack of knowledge on MeENPs impact on these important primary producers, the effects of Copper Oxide (CuO) ENPs on growth, physiology, pigment profiles, fatty acid (FA) metabolism, and oxidative stress were investigated in the model diatom Pheodactylum tricornutum, to provide suitable biomarkers of CuO ENP exposure versus its ionic counterpart. Diatom growth was inhibited by CuO ENPs but not Ionic Cu, suggesting CuO ENP cytotoxicity. Pulse Modulated Amplitude (PAM) phenotyping evidenced a decrease in the electron transport energy flux, pointing to a reduction in chemical energy generation following CuO ENPs exposure, as well as an increase in the content of the non-functional Cu-substituted chlorophyll a (CuChl a). A significant decrease in eicosapentaenoic acid (C20:5) associated with a significant rise in thylakoid membranes FAs reflected the activation of counteractive measures to photosynthetic impairment. Significant increase in the omega 6/omega 3 ratio, underline expectable negative repercussions to marine food webs. Increased thiobarbituric acid reactive substances reflected heightened oxidative stress by CuO ENP. Enhanced Glutathione Reductase and Ascorbate Peroxidase activity were also more evident for CuO ENPs than ionic Cu. Overall, observed molecular changes highlighted a battery of possible suitable biomarkers to efficiently determine the harmful effects of CuO ENPs. The results suggest that the occurrence and contamination of these new forms of metal contaminants can impose added stress to the marine diatom community, which could have significant impacts on marine ecosystems, namely through a reduction of the primary productivity, oxygen production and omega 6 production, all essential to sustain heterotrophic marine life.https://www.frontiersin.org/articles/10.3389/fmars.2020.539827/fullCuO nanoparticlesphotobiologyoxidative stresslipid metabolismphytoplanktoncytotoxicity |
| spellingShingle | Marco Franzitta Marco Franzitta Eduardo Feijão Eduardo Feijão Maria Teresa Cabrita Carla Gameiro Carla Gameiro Ana Rita Matos Ana Rita Matos João Carlos Marques Johannes W. Goessling Patrick Reis-Santos Patrick Reis-Santos Vanessa F. Fonseca Vanessa F. Fonseca Carlo Pretti Isabel Caçador Isabel Caçador Bernardo Duarte Bernardo Duarte Toxicity Going Nano: Ionic Versus Engineered Cu Nanoparticles Impacts on the Physiological Fitness of the Model Diatom Phaeodactylum tricornutum Frontiers in Marine Science CuO nanoparticles photobiology oxidative stress lipid metabolism phytoplankton cytotoxicity |
| title | Toxicity Going Nano: Ionic Versus Engineered Cu Nanoparticles Impacts on the Physiological Fitness of the Model Diatom Phaeodactylum tricornutum |
| title_full | Toxicity Going Nano: Ionic Versus Engineered Cu Nanoparticles Impacts on the Physiological Fitness of the Model Diatom Phaeodactylum tricornutum |
| title_fullStr | Toxicity Going Nano: Ionic Versus Engineered Cu Nanoparticles Impacts on the Physiological Fitness of the Model Diatom Phaeodactylum tricornutum |
| title_full_unstemmed | Toxicity Going Nano: Ionic Versus Engineered Cu Nanoparticles Impacts on the Physiological Fitness of the Model Diatom Phaeodactylum tricornutum |
| title_short | Toxicity Going Nano: Ionic Versus Engineered Cu Nanoparticles Impacts on the Physiological Fitness of the Model Diatom Phaeodactylum tricornutum |
| title_sort | toxicity going nano ionic versus engineered cu nanoparticles impacts on the physiological fitness of the model diatom phaeodactylum tricornutum |
| topic | CuO nanoparticles photobiology oxidative stress lipid metabolism phytoplankton cytotoxicity |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2020.539827/full |
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