Effect of Ethanolic Extract of <i>Vernonia amygdalina</i> on the Proliferation, Viability and Function of Mouse Induced Pluripotent Stem Cells and Cardiomyocytes
<i>Vernonia amygdalina</i> (<i>V. amygdalina</i>) leaves are commonly used in traditional medicine around the world for the treatment of a plethora disorders, including heart disease. The aim of this study was to examine and evaluate the cardiac effect of <i>V. amygdali...
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MDPI AG
2023-03-01
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| Online Access: | https://www.mdpi.com/2223-7747/12/5/1108 |
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| author | Arlette Nguemfo Tchankugni Mpoame Mbida Jürgen Hescheler Filomain Nguemo |
| author_facet | Arlette Nguemfo Tchankugni Mpoame Mbida Jürgen Hescheler Filomain Nguemo |
| author_sort | Arlette Nguemfo Tchankugni |
| collection | DOAJ |
| description | <i>Vernonia amygdalina</i> (<i>V. amygdalina</i>) leaves are commonly used in traditional medicine around the world for the treatment of a plethora disorders, including heart disease. The aim of this study was to examine and evaluate the cardiac effect of <i>V. amygdalina</i> leaf extracts using mouse induced pluripotent stem cells (miPSCs) and their cardiomyocytes’ (CMs) derivatives. We used a well-established stem cell culture to assess the effect of <i>V. amygdalina</i> extract on miPSC proliferation, EB formation and the beating activity of miPS cell-derived CMs. To study the cytotoxic effect of our extract, undifferentiating miPSCs were exposed to different concentrations of <i>V. amygdalina</i>. Cell colony formation and EB morphology were assessed using microscopy, whereas the cell viability was accessed with an impedance-based method and immunocytochemistry following treatment with different concentrations of <i>V. amygdalina</i>. Ethanolic extract of <i>V. amygdalina</i> induced toxicity in miPSCs, as revealed by a decrease in cell proliferation and colony formation, and an increase in cell death at a concentration of ≥20 mg/mL. At a concentration of 10 mg/mL, the rate of beating EBs was observed with no significant difference regarding the yield of cardiac cells. In addition, <i>V. amygdalina</i> did not affect the sarcomeric organization, but induced positive or negative effects on miPS cell-derived CMs’ differentiation in a concentration-dependent manner. Taken together, our findings demonstrate that the ethanolic extract of <i>V. amygdalina</i> affected cell proliferation, colony forming and cardiac beating capacities in a concentration-dependent manner. |
| first_indexed | 2024-03-11T07:13:30Z |
| format | Article |
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| language | English |
| last_indexed | 2024-03-11T07:13:30Z |
| publishDate | 2023-03-01 |
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| record_format | Article |
| series | Plants |
| spelling | doaj.art-480dddb104bd488d909ecaa64576e4f62023-11-17T08:24:15ZengMDPI AGPlants2223-77472023-03-01125110810.3390/plants12051108Effect of Ethanolic Extract of <i>Vernonia amygdalina</i> on the Proliferation, Viability and Function of Mouse Induced Pluripotent Stem Cells and CardiomyocytesArlette Nguemfo Tchankugni0Mpoame Mbida1Jürgen Hescheler2Filomain Nguemo3Research Unit of Biology and Applied Ecology, Department of Animal Biology, Faculty of Science, University of Dschang, Dschang P.O. Box 067, CameroonResearch Unit of Biology and Applied Ecology, Department of Animal Biology, Faculty of Science, University of Dschang, Dschang P.O. Box 067, CameroonCenter for Physiology and Pathophysiology, Faculty of Medicine and University Hospital Cologne, University of Cologne, 50931 Cologne, GermanyCenter for Physiology and Pathophysiology, Faculty of Medicine and University Hospital Cologne, University of Cologne, 50931 Cologne, Germany<i>Vernonia amygdalina</i> (<i>V. amygdalina</i>) leaves are commonly used in traditional medicine around the world for the treatment of a plethora disorders, including heart disease. The aim of this study was to examine and evaluate the cardiac effect of <i>V. amygdalina</i> leaf extracts using mouse induced pluripotent stem cells (miPSCs) and their cardiomyocytes’ (CMs) derivatives. We used a well-established stem cell culture to assess the effect of <i>V. amygdalina</i> extract on miPSC proliferation, EB formation and the beating activity of miPS cell-derived CMs. To study the cytotoxic effect of our extract, undifferentiating miPSCs were exposed to different concentrations of <i>V. amygdalina</i>. Cell colony formation and EB morphology were assessed using microscopy, whereas the cell viability was accessed with an impedance-based method and immunocytochemistry following treatment with different concentrations of <i>V. amygdalina</i>. Ethanolic extract of <i>V. amygdalina</i> induced toxicity in miPSCs, as revealed by a decrease in cell proliferation and colony formation, and an increase in cell death at a concentration of ≥20 mg/mL. At a concentration of 10 mg/mL, the rate of beating EBs was observed with no significant difference regarding the yield of cardiac cells. In addition, <i>V. amygdalina</i> did not affect the sarcomeric organization, but induced positive or negative effects on miPS cell-derived CMs’ differentiation in a concentration-dependent manner. Taken together, our findings demonstrate that the ethanolic extract of <i>V. amygdalina</i> affected cell proliferation, colony forming and cardiac beating capacities in a concentration-dependent manner.https://www.mdpi.com/2223-7747/12/5/1108natural productsmedicinal plants<i>Vernonia amygdalina</i>pluripotent stem cellscardiomyocytescytotoxicity |
| spellingShingle | Arlette Nguemfo Tchankugni Mpoame Mbida Jürgen Hescheler Filomain Nguemo Effect of Ethanolic Extract of <i>Vernonia amygdalina</i> on the Proliferation, Viability and Function of Mouse Induced Pluripotent Stem Cells and Cardiomyocytes Plants natural products medicinal plants <i>Vernonia amygdalina</i> pluripotent stem cells cardiomyocytes cytotoxicity |
| title | Effect of Ethanolic Extract of <i>Vernonia amygdalina</i> on the Proliferation, Viability and Function of Mouse Induced Pluripotent Stem Cells and Cardiomyocytes |
| title_full | Effect of Ethanolic Extract of <i>Vernonia amygdalina</i> on the Proliferation, Viability and Function of Mouse Induced Pluripotent Stem Cells and Cardiomyocytes |
| title_fullStr | Effect of Ethanolic Extract of <i>Vernonia amygdalina</i> on the Proliferation, Viability and Function of Mouse Induced Pluripotent Stem Cells and Cardiomyocytes |
| title_full_unstemmed | Effect of Ethanolic Extract of <i>Vernonia amygdalina</i> on the Proliferation, Viability and Function of Mouse Induced Pluripotent Stem Cells and Cardiomyocytes |
| title_short | Effect of Ethanolic Extract of <i>Vernonia amygdalina</i> on the Proliferation, Viability and Function of Mouse Induced Pluripotent Stem Cells and Cardiomyocytes |
| title_sort | effect of ethanolic extract of i vernonia amygdalina i on the proliferation viability and function of mouse induced pluripotent stem cells and cardiomyocytes |
| topic | natural products medicinal plants <i>Vernonia amygdalina</i> pluripotent stem cells cardiomyocytes cytotoxicity |
| url | https://www.mdpi.com/2223-7747/12/5/1108 |
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