Antioxidant Activity and Teratogenicity Evaluation of Lawsonia Inermis in BALB/c Mice
Background and Aim: Lawsonia inermis is a medicinal plant with abortive properties. There has been no scientific study to evaluate the teratogenicity of this plant. This study was performed to determine the antioxidant activity and the possible side effect of L. inermis hydroalcoholic extract on...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2015-05-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://jcdr.net/articles/PDF/5911/12290_CE[Ra]_F(P)_PF1(AGAK)_PFA(P)_PF2(PAG).pdf |
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| author | Lobat Jafarzadeh Neda Seifi Najmeh Shahinfard Mehrnoosh Sedighi Soleiman Kheiri Hedayatollah Shirzad Mahmoud Rafieian-Kopaei |
| author_facet | Lobat Jafarzadeh Neda Seifi Najmeh Shahinfard Mehrnoosh Sedighi Soleiman Kheiri Hedayatollah Shirzad Mahmoud Rafieian-Kopaei |
| author_sort | Lobat Jafarzadeh |
| collection | DOAJ |
| description | Background and Aim: Lawsonia inermis is a medicinal plant
with abortive properties. There has been no scientific study
to evaluate the teratogenicity of this plant. This study was
performed to determine the antioxidant activity and the possible
side effect of L. inermis hydroalcoholic extract on development
of congenital abnormalities in BALB/c mice.
Materials and Methods: In this experimental study, 120 female
mature BALB/c mice were assigned to four groups and after
mating and confirming the vaginal plug, the animals in the first
group (G1) were kept with no intervention, and the second (G2),
third (G3) and fourth (G4) groups were intraperitoneally (ip)
injected with respectively saline (0.3 ml), and 10 and 100 mg/
kg of L. inermis extract (for 7 days). On the 19th day, caesarean
section was performed on the mice and embryos were examined
for abnormalities. Their height and weight were measured.
Data were analysed by ANOVA and post-hoc least significant
difference tests.
Results: There were significant differences between G3 and G4,
and G1 (p<0.001); no significant difference was seen between
G3 and G4. At 100 mg/kg dose of L. inermis, the parietal bones
were absent in 90% of embryos and more extra ribs were
observed in both G3 and G4 (p = 0.01).
Conclusion: L. inermis may have teratogenicity and should be
used cautiously during pregnancy. |
| first_indexed | 2024-12-12T21:53:31Z |
| format | Article |
| id | doaj.art-48125dc25c7149299b250855c1244d26 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-12T21:53:31Z |
| publishDate | 2015-05-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-48125dc25c7149299b250855c1244d262022-12-22T00:10:44ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2015-05-0195FF01FF0410.7860/JCDR/2015/12290.5911Antioxidant Activity and Teratogenicity Evaluation of Lawsonia Inermis in BALB/c MiceLobat Jafarzadeh0Neda Seifi1Najmeh Shahinfard2Mehrnoosh Sedighi3Soleiman Kheiri4Hedayatollah Shirzad5Mahmoud Rafieian-Kopaei6Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.Background and Aim: Lawsonia inermis is a medicinal plant with abortive properties. There has been no scientific study to evaluate the teratogenicity of this plant. This study was performed to determine the antioxidant activity and the possible side effect of L. inermis hydroalcoholic extract on development of congenital abnormalities in BALB/c mice. Materials and Methods: In this experimental study, 120 female mature BALB/c mice were assigned to four groups and after mating and confirming the vaginal plug, the animals in the first group (G1) were kept with no intervention, and the second (G2), third (G3) and fourth (G4) groups were intraperitoneally (ip) injected with respectively saline (0.3 ml), and 10 and 100 mg/ kg of L. inermis extract (for 7 days). On the 19th day, caesarean section was performed on the mice and embryos were examined for abnormalities. Their height and weight were measured. Data were analysed by ANOVA and post-hoc least significant difference tests. Results: There were significant differences between G3 and G4, and G1 (p<0.001); no significant difference was seen between G3 and G4. At 100 mg/kg dose of L. inermis, the parietal bones were absent in 90% of embryos and more extra ribs were observed in both G3 and G4 (p = 0.01). Conclusion: L. inermis may have teratogenicity and should be used cautiously during pregnancy.https://jcdr.net/articles/PDF/5911/12290_CE[Ra]_F(P)_PF1(AGAK)_PFA(P)_PF2(PAG).pdfherbal medicinesmedicinal plantpregnancysafetytoxicity |
| spellingShingle | Lobat Jafarzadeh Neda Seifi Najmeh Shahinfard Mehrnoosh Sedighi Soleiman Kheiri Hedayatollah Shirzad Mahmoud Rafieian-Kopaei Antioxidant Activity and Teratogenicity Evaluation of Lawsonia Inermis in BALB/c Mice Journal of Clinical and Diagnostic Research herbal medicines medicinal plant pregnancy safety toxicity |
| title | Antioxidant Activity and Teratogenicity Evaluation of Lawsonia Inermis in BALB/c Mice |
| title_full | Antioxidant Activity and Teratogenicity Evaluation of Lawsonia Inermis in BALB/c Mice |
| title_fullStr | Antioxidant Activity and Teratogenicity Evaluation of Lawsonia Inermis in BALB/c Mice |
| title_full_unstemmed | Antioxidant Activity and Teratogenicity Evaluation of Lawsonia Inermis in BALB/c Mice |
| title_short | Antioxidant Activity and Teratogenicity Evaluation of Lawsonia Inermis in BALB/c Mice |
| title_sort | antioxidant activity and teratogenicity evaluation of lawsonia inermis in balb c mice |
| topic | herbal medicines medicinal plant pregnancy safety toxicity |
| url | https://jcdr.net/articles/PDF/5911/12290_CE[Ra]_F(P)_PF1(AGAK)_PFA(P)_PF2(PAG).pdf |
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