First report of Mansonella sp. and Dipetalonema gracile in the Amazonian city-dwelling threatened primate, Saguinus bicolor
The pied tamarin, or Saguinus bicolor, is a callitrichid that inhabits Amazon Forest fragments encased within the municipalities of Manaus, Rio Preto da Eva, and Itacoatiara and their outskirts. Therefore, this primate lives in great proximity to humans, and is in critical danger of extinction, resu...
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Frontiers Media S.A.
2023-12-01
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| Series: | Frontiers in Tropical Diseases |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fitd.2023.1080218/full |
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| author | Cindy Alves Dias Cindy Alves Dias Túllio Romão Ribeiro da Silva Marcelo Gordo David Marcial Fernandez Conga Natália Aparecida de Souza Lima Aline Souza de Menezes Medeiros Edson Rodrigues Costa Sérgio Luiz Bessa Luz Carlos Henrique Aguiar Costa Carlos Henrique Aguiar Costa Carlos Henrique Aguiar Costa Ana Carolina Paulo Vicente Thaís Pinto Nascimento Thaís Pinto Nascimento Francisca Helena Aguiar-Silva Francisca Helena Aguiar-Silva Viviane Costa da Silva Viviane Costa da Silva Diogo César Lagroteria Laerzio Chiesorin Neto Laerzio Chiesorin Neto Alessandra Ferreira Dales Nava |
| author_facet | Cindy Alves Dias Cindy Alves Dias Túllio Romão Ribeiro da Silva Marcelo Gordo David Marcial Fernandez Conga Natália Aparecida de Souza Lima Aline Souza de Menezes Medeiros Edson Rodrigues Costa Sérgio Luiz Bessa Luz Carlos Henrique Aguiar Costa Carlos Henrique Aguiar Costa Carlos Henrique Aguiar Costa Ana Carolina Paulo Vicente Thaís Pinto Nascimento Thaís Pinto Nascimento Francisca Helena Aguiar-Silva Francisca Helena Aguiar-Silva Viviane Costa da Silva Viviane Costa da Silva Diogo César Lagroteria Laerzio Chiesorin Neto Laerzio Chiesorin Neto Alessandra Ferreira Dales Nava |
| author_sort | Cindy Alves Dias |
| collection | DOAJ |
| description | The pied tamarin, or Saguinus bicolor, is a callitrichid that inhabits Amazon Forest fragments encased within the municipalities of Manaus, Rio Preto da Eva, and Itacoatiara and their outskirts. Therefore, this primate lives in great proximity to humans, and is in critical danger of extinction, resulting from ongoing anthropogenic pressures, with habitat fragmentation being the most prominent threat. Greater conservation efforts and more studies concerning public health need to be carried out in this situation, such as the study of infectious diseases that can affect this primate, including those involving helminths. In this study, we combined necropsy, microscopy with blood smears and quick Panoptic stains, and molecular methods like nested polymerase chain reaction (PCR) targeting the internal transcribed spacer-1 (ITS-1) region, Sanger sequencing and shotgun sequencing to detect and identify filarial parasites in 71 S. bicolor samples. We detected 24 adult filarial worms in 6.45% of the thoracic cavities, microfilaria in 6.38% from blood smears, and filarial DNA in 28.57% positive blood samples via PCR. We identified eight of the adult worms as being from the Onchocercidae family using Sanger sequencing and one specifically as Dipetalonema gracile, using shotgun sequencing. For the positive blood samples, 70.58% of them were for Mansonella sp., 17.64% for Dipetalonema sp., and 11.76% could only be identified as belonging to the Onchocercidae family. There was an event of coinfection that involved Dipetalonema sp. adult worm and Mansonella sp. microfilaria. This is the first report of the detection of Dipetalonema gracile and the genus Mansonella in S. bicolor, as well as an event of coinfection, pointing out this primate as a new host. It is also another step to understand the situation of filarial infections occurring in Amazonian Regions and its municipalities. |
| first_indexed | 2024-03-08T22:06:06Z |
| format | Article |
| id | doaj.art-4812e3ade7734575aa26cfdff9d8d394 |
| institution | Directory Open Access Journal |
| issn | 2673-7515 |
| language | English |
| last_indexed | 2024-03-08T22:06:06Z |
| publishDate | 2023-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Tropical Diseases |
| spelling | doaj.art-4812e3ade7734575aa26cfdff9d8d3942023-12-19T08:34:34ZengFrontiers Media S.A.Frontiers in Tropical Diseases2673-75152023-12-01410.3389/fitd.2023.10802181080218First report of Mansonella sp. and Dipetalonema gracile in the Amazonian city-dwelling threatened primate, Saguinus bicolorCindy Alves Dias0Cindy Alves Dias1Túllio Romão Ribeiro da Silva2Marcelo Gordo3David Marcial Fernandez Conga4Natália Aparecida de Souza Lima5Aline Souza de Menezes Medeiros6Edson Rodrigues Costa7Sérgio Luiz Bessa Luz8Carlos Henrique Aguiar Costa9Carlos Henrique Aguiar Costa10Carlos Henrique Aguiar Costa11Ana Carolina Paulo Vicente12Thaís Pinto Nascimento13Thaís Pinto Nascimento14Francisca Helena Aguiar-Silva15Francisca Helena Aguiar-Silva16Viviane Costa da Silva17Viviane Costa da Silva18Diogo César Lagroteria19Laerzio Chiesorin Neto20Laerzio Chiesorin Neto21Alessandra Ferreira Dales Nava22Laboratório de Ecologia de Doenças Transmissíveis na Amazônia, Instituto Leônidas e Maria Deane (ILMD)/Fiocruz Amazônia, Manaus, BrazilPrograma de Pós-Graduação em Biologia da Interação Patógeno Hospedeiro (PPGBIO-Interação), Instituto Leônidas e Maria Deane/ILMD/Fiocruz Amazônia, Manaus, AM, BrazilLaboratório de Ecologia de Doenças Transmissíveis na Amazônia, Instituto Leônidas e Maria Deane (ILMD)/Fiocruz Amazônia, Manaus, BrazilLaboratório de Biologia da Conservação, Projeto Sauim-de-Coleira, PPGZOO, PPGCASA, CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) DB/ICB UFAM, Manaus, BrazilPrograma de Pós-Graduação em Saúde e Produção Animal na Amazônia, UFRA, Belém, BrazilCentro de Triagem de Animais Silvestres, IBAMA/SUPES/AM, Manaus, BrazilLaboratório de Biologia da Conservação, Projeto Sauim-de-Coleira, PPGZOO, PPGCASA, CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) DB/ICB UFAM, Manaus, BrazilLaboratório de Biologia da Conservação, Projeto Sauim-de-Coleira, PPGZOO, PPGCASA, CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) DB/ICB UFAM, Manaus, BrazilLaboratório de Ecologia de Doenças Transmissíveis na Amazônia, Instituto Leônidas e Maria Deane (ILMD)/Fiocruz Amazônia, Manaus, BrazilLaboratório de Ecologia de Doenças Transmissíveis na Amazônia, Instituto Leônidas e Maria Deane (ILMD)/Fiocruz Amazônia, Manaus, BrazilInstituto Oswaldo Cruz (IOC), Fiocruz, Laboratório de Genética Molecular de Microrganismos, Rio de Janeiro, BrazilPrograma de Pós-Graduação Stricto Sensu em Biologia Computacional e Sistemas (PGBCS-IOC), Instituto Oswaldo Cruz (IOC), Rio de Janeiro, BrazilInstituto Oswaldo Cruz (IOC), Fiocruz, Laboratório de Genética Molecular de Microrganismos, Rio de Janeiro, BrazilLaboratório de Ecologia de Doenças Transmissíveis na Amazônia, Instituto Leônidas e Maria Deane (ILMD)/Fiocruz Amazônia, Manaus, BrazilPrograma de Pós-Graduação em Biologia da Interação Patógeno Hospedeiro (PPGBIO-Interação), Instituto Leônidas e Maria Deane/ILMD/Fiocruz Amazônia, Manaus, AM, BrazilLaboratório de Ecologia de Doenças Transmissíveis na Amazônia, Instituto Leônidas e Maria Deane (ILMD)/Fiocruz Amazônia, Manaus, BrazilFundação de Amparo à Pesquisa do Estado do Amazonas (FAPEAM), Manaus, BrazilLaboratório de Ecologia de Doenças Transmissíveis na Amazônia, Instituto Leônidas e Maria Deane (ILMD)/Fiocruz Amazônia, Manaus, BrazilFundação de Amparo à Pesquisa do Estado do Amazonas (FAPEAM), Manaus, BrazilCentro Nacional de Pesquisa e Conservação da Biodiversidade Amazônica, ICMBio/CEPAM, Manaus, BrazilCentro de Triagem de Animais Silvestres, IBAMA/SUPES/AM, Manaus, Brazil0Uniniltonlins, Manaus, BrazilLaboratório de Ecologia de Doenças Transmissíveis na Amazônia, Instituto Leônidas e Maria Deane (ILMD)/Fiocruz Amazônia, Manaus, BrazilThe pied tamarin, or Saguinus bicolor, is a callitrichid that inhabits Amazon Forest fragments encased within the municipalities of Manaus, Rio Preto da Eva, and Itacoatiara and their outskirts. Therefore, this primate lives in great proximity to humans, and is in critical danger of extinction, resulting from ongoing anthropogenic pressures, with habitat fragmentation being the most prominent threat. Greater conservation efforts and more studies concerning public health need to be carried out in this situation, such as the study of infectious diseases that can affect this primate, including those involving helminths. In this study, we combined necropsy, microscopy with blood smears and quick Panoptic stains, and molecular methods like nested polymerase chain reaction (PCR) targeting the internal transcribed spacer-1 (ITS-1) region, Sanger sequencing and shotgun sequencing to detect and identify filarial parasites in 71 S. bicolor samples. We detected 24 adult filarial worms in 6.45% of the thoracic cavities, microfilaria in 6.38% from blood smears, and filarial DNA in 28.57% positive blood samples via PCR. We identified eight of the adult worms as being from the Onchocercidae family using Sanger sequencing and one specifically as Dipetalonema gracile, using shotgun sequencing. For the positive blood samples, 70.58% of them were for Mansonella sp., 17.64% for Dipetalonema sp., and 11.76% could only be identified as belonging to the Onchocercidae family. There was an event of coinfection that involved Dipetalonema sp. adult worm and Mansonella sp. microfilaria. This is the first report of the detection of Dipetalonema gracile and the genus Mansonella in S. bicolor, as well as an event of coinfection, pointing out this primate as a new host. It is also another step to understand the situation of filarial infections occurring in Amazonian Regions and its municipalities.https://www.frontiersin.org/articles/10.3389/fitd.2023.1080218/fullpied tamarinAmazon rainforestcallitrichidfilarianematodaconservation |
| spellingShingle | Cindy Alves Dias Cindy Alves Dias Túllio Romão Ribeiro da Silva Marcelo Gordo David Marcial Fernandez Conga Natália Aparecida de Souza Lima Aline Souza de Menezes Medeiros Edson Rodrigues Costa Sérgio Luiz Bessa Luz Carlos Henrique Aguiar Costa Carlos Henrique Aguiar Costa Carlos Henrique Aguiar Costa Ana Carolina Paulo Vicente Thaís Pinto Nascimento Thaís Pinto Nascimento Francisca Helena Aguiar-Silva Francisca Helena Aguiar-Silva Viviane Costa da Silva Viviane Costa da Silva Diogo César Lagroteria Laerzio Chiesorin Neto Laerzio Chiesorin Neto Alessandra Ferreira Dales Nava First report of Mansonella sp. and Dipetalonema gracile in the Amazonian city-dwelling threatened primate, Saguinus bicolor Frontiers in Tropical Diseases pied tamarin Amazon rainforest callitrichid filaria nematoda conservation |
| title | First report of Mansonella sp. and Dipetalonema gracile in the Amazonian city-dwelling threatened primate, Saguinus bicolor |
| title_full | First report of Mansonella sp. and Dipetalonema gracile in the Amazonian city-dwelling threatened primate, Saguinus bicolor |
| title_fullStr | First report of Mansonella sp. and Dipetalonema gracile in the Amazonian city-dwelling threatened primate, Saguinus bicolor |
| title_full_unstemmed | First report of Mansonella sp. and Dipetalonema gracile in the Amazonian city-dwelling threatened primate, Saguinus bicolor |
| title_short | First report of Mansonella sp. and Dipetalonema gracile in the Amazonian city-dwelling threatened primate, Saguinus bicolor |
| title_sort | first report of mansonella sp and dipetalonema gracile in the amazonian city dwelling threatened primate saguinus bicolor |
| topic | pied tamarin Amazon rainforest callitrichid filaria nematoda conservation |
| url | https://www.frontiersin.org/articles/10.3389/fitd.2023.1080218/full |
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