Evaluation of Cellulase Production by Zymomonas mobilis
Z. mobilis has been widely studied as a potential microbe for consolidated bioprocessing to convert lignocellulosic biomass to fermentable sugars while at the same time producing ethanol. To achieve this goal, Z. mobilis must be evaluated for the production of cellulolytic enzyme. This work reports...
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| Format: | Article |
| Language: | English |
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North Carolina State University
2016-12-01
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| Series: | BioResources |
| Subjects: | |
| Online Access: | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_12_1_1165_Todhanakasem_Cellulase_Production_Zymomonas |
| _version_ | 1818458737800642560 |
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| author | Tatsaporn Todhanakasem Siripa Jittjang |
| author_facet | Tatsaporn Todhanakasem Siripa Jittjang |
| author_sort | Tatsaporn Todhanakasem |
| collection | DOAJ |
| description | Z. mobilis has been widely studied as a potential microbe for consolidated bioprocessing to convert lignocellulosic biomass to fermentable sugars while at the same time producing ethanol. To achieve this goal, Z. mobilis must be evaluated for the production of cellulolytic enzyme. This work reports on the potential of intracellular and extracellular crude extracts from Z. mobilis ZM4 and TISTR 551 to hydrolyze various cellulosic materials including carboxymethylcellulose (CMC), delignified rice bran, microcrystalline cellulose, and filter paper. Crude intracellular extracts from ZM4 and TISTR 551 showed high endoglucanase activity with CMC substrates at an optimal pH of 6 to 7 and temperature range of 30 to 40 °C. The endoglucanase activity from the crude extracts was significantly higher than the exoglucanase activity. Of the high crystalline celluloses substrates tested, the best results were obtained for the hydrolysis of delignified rice bran by crude intracellular enzyme extracts of Z. mobilis TISTR 551. |
| first_indexed | 2024-12-14T23:03:13Z |
| format | Article |
| id | doaj.art-4813c442e9ed4d5898f0616d8cd68b10 |
| institution | Directory Open Access Journal |
| issn | 1930-2126 1930-2126 |
| language | English |
| last_indexed | 2024-12-14T23:03:13Z |
| publishDate | 2016-12-01 |
| publisher | North Carolina State University |
| record_format | Article |
| series | BioResources |
| spelling | doaj.art-4813c442e9ed4d5898f0616d8cd68b102022-12-21T22:44:24ZengNorth Carolina State UniversityBioResources1930-21261930-21262016-12-011211165117810.15376/biores.12.1.1165-1178Evaluation of Cellulase Production by Zymomonas mobilisTatsaporn Todhanakasem0Siripa Jittjang1Department of Agro- Industry, Assumption University, Thailand; ThailandDepartment of Agro- Industry, Assumption University; Tanzania, United Republic OfZ. mobilis has been widely studied as a potential microbe for consolidated bioprocessing to convert lignocellulosic biomass to fermentable sugars while at the same time producing ethanol. To achieve this goal, Z. mobilis must be evaluated for the production of cellulolytic enzyme. This work reports on the potential of intracellular and extracellular crude extracts from Z. mobilis ZM4 and TISTR 551 to hydrolyze various cellulosic materials including carboxymethylcellulose (CMC), delignified rice bran, microcrystalline cellulose, and filter paper. Crude intracellular extracts from ZM4 and TISTR 551 showed high endoglucanase activity with CMC substrates at an optimal pH of 6 to 7 and temperature range of 30 to 40 °C. The endoglucanase activity from the crude extracts was significantly higher than the exoglucanase activity. Of the high crystalline celluloses substrates tested, the best results were obtained for the hydrolysis of delignified rice bran by crude intracellular enzyme extracts of Z. mobilis TISTR 551.http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_12_1_1165_Todhanakasem_Cellulase_Production_ZymomonasConsolidated bioprocess; Zymomonas mobilis; Cellulase; Lignocellulosic material |
| spellingShingle | Tatsaporn Todhanakasem Siripa Jittjang Evaluation of Cellulase Production by Zymomonas mobilis BioResources Consolidated bioprocess; Zymomonas mobilis; Cellulase; Lignocellulosic material |
| title | Evaluation of Cellulase Production by Zymomonas mobilis |
| title_full | Evaluation of Cellulase Production by Zymomonas mobilis |
| title_fullStr | Evaluation of Cellulase Production by Zymomonas mobilis |
| title_full_unstemmed | Evaluation of Cellulase Production by Zymomonas mobilis |
| title_short | Evaluation of Cellulase Production by Zymomonas mobilis |
| title_sort | evaluation of cellulase production by zymomonas mobilis |
| topic | Consolidated bioprocess; Zymomonas mobilis; Cellulase; Lignocellulosic material |
| url | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_12_1_1165_Todhanakasem_Cellulase_Production_Zymomonas |
| work_keys_str_mv | AT tatsaporntodhanakasem evaluationofcellulaseproductionbyzymomonasmobilis AT siripajittjang evaluationofcellulaseproductionbyzymomonasmobilis |