Biogenic synthesis, characterization of antibacterial silver nanoparticles and its cell cytotoxicity
The advanced research and development of silver nanoparticles (AgNPs) is vast due to their incredible applications today. In this work, AgNPs were synthesized using soil derived Pseudomonas putida MVP2. The AgNPs formation on the P. putida cell membrane and its cell free supernatant was studied. The...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2017-12-01
|
| Series: | Arabian Journal of Chemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1878535215003238 |
| _version_ | 1818037876915437568 |
|---|---|
| author | V. Gopinath S. Priyadarshini Mun Fai Loke J. Arunkumar Enrico Marsili D. MubarakAli P. Velusamy Jamuna Vadivelu |
| author_facet | V. Gopinath S. Priyadarshini Mun Fai Loke J. Arunkumar Enrico Marsili D. MubarakAli P. Velusamy Jamuna Vadivelu |
| author_sort | V. Gopinath |
| collection | DOAJ |
| description | The advanced research and development of silver nanoparticles (AgNPs) is vast due to their incredible applications today. In this work, AgNPs were synthesized using soil derived Pseudomonas putida MVP2. The AgNPs formation on the P. putida cell membrane and its cell free supernatant was studied. The synthesized AgNPs were characterized by UV–visible spectroscopy, scanning transmission electron microscopy (STEM), X-ray diffraction (XRD), energy dispersive X-ray (EDAX) and Fourier transform infrared (FTIR) spectrum analysis. The mode of action of AgNPs on the bacteria was studied against clinically isolated bacterial pathogens, Staphylococcus aureus, Escherichia coli, Bacillus cereus, Pseudomonas aeruginosa and Helicobacter pylori by membrane integrity, and protein leakage using confocal and electron microscopy. Interestingly, AgNPs had no cytotoxicity under 25 μg/mL and it was toxic at above 50 μg/mL on human epidermoid larynx carcinoma (HEp-2) cells. This study evidenced that biogenic nanoparticles could affect the bacterial replication, protein leakage and eventually cell death. This might be used for active antimicrobial agents for the chronic infections. |
| first_indexed | 2024-12-10T07:33:49Z |
| format | Article |
| id | doaj.art-481422adfa3a4a80897859d27200644a |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-12-10T07:33:49Z |
| publishDate | 2017-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-481422adfa3a4a80897859d27200644a2022-12-22T01:57:29ZengElsevierArabian Journal of Chemistry1878-53522017-12-011081107111710.1016/j.arabjc.2015.11.011Biogenic synthesis, characterization of antibacterial silver nanoparticles and its cell cytotoxicityV. Gopinath0S. Priyadarshini1Mun Fai Loke2J. Arunkumar3Enrico Marsili4D. MubarakAli5P. Velusamy6Jamuna Vadivelu7Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Chennai 603 203, Tamil Nadu, IndiaDepartment of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, MalaysiaDepartment of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, MalaysiaDepartment of Microbiology, SRM University, Sonepat, Haryana 131029, IndiaSingapore Centre for Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, 637551, SingaporeDivision of Bioengineering, Incheon National University, Incheon, Republic of KoreaDepartment of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Chennai 603 203, Tamil Nadu, IndiaDepartment of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, MalaysiaThe advanced research and development of silver nanoparticles (AgNPs) is vast due to their incredible applications today. In this work, AgNPs were synthesized using soil derived Pseudomonas putida MVP2. The AgNPs formation on the P. putida cell membrane and its cell free supernatant was studied. The synthesized AgNPs were characterized by UV–visible spectroscopy, scanning transmission electron microscopy (STEM), X-ray diffraction (XRD), energy dispersive X-ray (EDAX) and Fourier transform infrared (FTIR) spectrum analysis. The mode of action of AgNPs on the bacteria was studied against clinically isolated bacterial pathogens, Staphylococcus aureus, Escherichia coli, Bacillus cereus, Pseudomonas aeruginosa and Helicobacter pylori by membrane integrity, and protein leakage using confocal and electron microscopy. Interestingly, AgNPs had no cytotoxicity under 25 μg/mL and it was toxic at above 50 μg/mL on human epidermoid larynx carcinoma (HEp-2) cells. This study evidenced that biogenic nanoparticles could affect the bacterial replication, protein leakage and eventually cell death. This might be used for active antimicrobial agents for the chronic infections.http://www.sciencedirect.com/science/article/pii/S1878535215003238BiosynthesisPseudomonas putidaAntimicrobial activityCytotoxicityBiocompatibilitySilver nanoparticles |
| spellingShingle | V. Gopinath S. Priyadarshini Mun Fai Loke J. Arunkumar Enrico Marsili D. MubarakAli P. Velusamy Jamuna Vadivelu Biogenic synthesis, characterization of antibacterial silver nanoparticles and its cell cytotoxicity Arabian Journal of Chemistry Biosynthesis Pseudomonas putida Antimicrobial activity Cytotoxicity Biocompatibility Silver nanoparticles |
| title | Biogenic synthesis, characterization of antibacterial silver nanoparticles and its cell cytotoxicity |
| title_full | Biogenic synthesis, characterization of antibacterial silver nanoparticles and its cell cytotoxicity |
| title_fullStr | Biogenic synthesis, characterization of antibacterial silver nanoparticles and its cell cytotoxicity |
| title_full_unstemmed | Biogenic synthesis, characterization of antibacterial silver nanoparticles and its cell cytotoxicity |
| title_short | Biogenic synthesis, characterization of antibacterial silver nanoparticles and its cell cytotoxicity |
| title_sort | biogenic synthesis characterization of antibacterial silver nanoparticles and its cell cytotoxicity |
| topic | Biosynthesis Pseudomonas putida Antimicrobial activity Cytotoxicity Biocompatibility Silver nanoparticles |
| url | http://www.sciencedirect.com/science/article/pii/S1878535215003238 |
| work_keys_str_mv | AT vgopinath biogenicsynthesischaracterizationofantibacterialsilvernanoparticlesanditscellcytotoxicity AT spriyadarshini biogenicsynthesischaracterizationofantibacterialsilvernanoparticlesanditscellcytotoxicity AT munfailoke biogenicsynthesischaracterizationofantibacterialsilvernanoparticlesanditscellcytotoxicity AT jarunkumar biogenicsynthesischaracterizationofantibacterialsilvernanoparticlesanditscellcytotoxicity AT enricomarsili biogenicsynthesischaracterizationofantibacterialsilvernanoparticlesanditscellcytotoxicity AT dmubarakali biogenicsynthesischaracterizationofantibacterialsilvernanoparticlesanditscellcytotoxicity AT pvelusamy biogenicsynthesischaracterizationofantibacterialsilvernanoparticlesanditscellcytotoxicity AT jamunavadivelu biogenicsynthesischaracterizationofantibacterialsilvernanoparticlesanditscellcytotoxicity |