PSMD12 promotes the activation of the MEK-ERK pathway by upregulating KIF15 to promote the malignant progression of liver cancer
The tumor recurrence and drug resistance of hepatocellular carcinoma (HCC) threatened patients a lot. The mechanism should be further explored. The information of expression status and survival were available in public databases. The Western blot and immunohistochemistry staining displayed the level...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-12-01
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| Series: | Cancer Biology & Therapy |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/15384047.2022.2125260 |
| _version_ | 1797404030531534848 |
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| author | Hanpu Zhang Chenyuan Li Shichong Liao Yi Tu Shengrong Sun Feng Yao Zhiyu Li Zhong Wang |
| author_facet | Hanpu Zhang Chenyuan Li Shichong Liao Yi Tu Shengrong Sun Feng Yao Zhiyu Li Zhong Wang |
| author_sort | Hanpu Zhang |
| collection | DOAJ |
| description | The tumor recurrence and drug resistance of hepatocellular carcinoma (HCC) threatened patients a lot. The mechanism should be further explored. The information of expression status and survival were available in public databases. The Western blot and immunohistochemistry staining displayed the level of related proteins. CCK-8, colony-formation assays, transwell assay and wound healing assay were performed to illustrate the ability of tumor growth, invasion and migration. In vivo model was established to verify our cell experiments. In our study, we revealed that proteasome 26S subunit, non-ATPase 12 (PSMD12) was high expressed in HCC tissues and positive related to the survival. In vitro experiments suggested that PSMD12 knockdown attenuated tumor cell growth, invasion and migration. Moreover, PSMD12 interference blocked the activation of MEK-ERK pathway. The ERK inhibitor could alleviate the tumor-promoting effect in PSMD12-overexpression cells. In addition, kinesin family member 15 (KIF15) was also observed to be highly expressed in HCC and be harmful to the survival. The public database, the images of immunohistochemistry and the western blot illustrated that PSMD12 and KIF15 was positive correlated. KIF15 knockdown impaired tumor progression and tumor-promoting effect of PSMD12. The xenograft models supported the results of cell experiments. In conclusion, PSMD12 could activated MEK-ERK pathway via KIF15 upregulation, thereby promoting tumor progression. |
| first_indexed | 2024-03-09T02:47:13Z |
| format | Article |
| id | doaj.art-4818dd66f2db46008a876e186a5932ab |
| institution | Directory Open Access Journal |
| issn | 1538-4047 1555-8576 |
| language | English |
| last_indexed | 2024-03-09T02:47:13Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Cancer Biology & Therapy |
| spelling | doaj.art-4818dd66f2db46008a876e186a5932ab2023-12-05T15:58:14ZengTaylor & Francis GroupCancer Biology & Therapy1538-40471555-85762022-12-0123111110.1080/15384047.2022.21252602125260PSMD12 promotes the activation of the MEK-ERK pathway by upregulating KIF15 to promote the malignant progression of liver cancerHanpu Zhang0Chenyuan Li1Shichong Liao2Yi Tu3Shengrong Sun4Feng Yao5Zhiyu Li6Zhong Wang7Renmin Hospital of Wuhan UniversityRenmin Hospital of Wuhan UniversityRenmin Hospital of Wuhan UniversityRenmin Hospital of Wuhan UniversityRenmin Hospital of Wuhan UniversityRenmin Hospital of Wuhan UniversityRenmin Hospital of Wuhan UniversityRenmin Hospital of Wuhan UniversityThe tumor recurrence and drug resistance of hepatocellular carcinoma (HCC) threatened patients a lot. The mechanism should be further explored. The information of expression status and survival were available in public databases. The Western blot and immunohistochemistry staining displayed the level of related proteins. CCK-8, colony-formation assays, transwell assay and wound healing assay were performed to illustrate the ability of tumor growth, invasion and migration. In vivo model was established to verify our cell experiments. In our study, we revealed that proteasome 26S subunit, non-ATPase 12 (PSMD12) was high expressed in HCC tissues and positive related to the survival. In vitro experiments suggested that PSMD12 knockdown attenuated tumor cell growth, invasion and migration. Moreover, PSMD12 interference blocked the activation of MEK-ERK pathway. The ERK inhibitor could alleviate the tumor-promoting effect in PSMD12-overexpression cells. In addition, kinesin family member 15 (KIF15) was also observed to be highly expressed in HCC and be harmful to the survival. The public database, the images of immunohistochemistry and the western blot illustrated that PSMD12 and KIF15 was positive correlated. KIF15 knockdown impaired tumor progression and tumor-promoting effect of PSMD12. The xenograft models supported the results of cell experiments. In conclusion, PSMD12 could activated MEK-ERK pathway via KIF15 upregulation, thereby promoting tumor progression.http://dx.doi.org/10.1080/15384047.2022.2125260psmd12kif15mekerkhepatocellular carcinoma |
| spellingShingle | Hanpu Zhang Chenyuan Li Shichong Liao Yi Tu Shengrong Sun Feng Yao Zhiyu Li Zhong Wang PSMD12 promotes the activation of the MEK-ERK pathway by upregulating KIF15 to promote the malignant progression of liver cancer Cancer Biology & Therapy psmd12 kif15 mek erk hepatocellular carcinoma |
| title | PSMD12 promotes the activation of the MEK-ERK pathway by upregulating KIF15 to promote the malignant progression of liver cancer |
| title_full | PSMD12 promotes the activation of the MEK-ERK pathway by upregulating KIF15 to promote the malignant progression of liver cancer |
| title_fullStr | PSMD12 promotes the activation of the MEK-ERK pathway by upregulating KIF15 to promote the malignant progression of liver cancer |
| title_full_unstemmed | PSMD12 promotes the activation of the MEK-ERK pathway by upregulating KIF15 to promote the malignant progression of liver cancer |
| title_short | PSMD12 promotes the activation of the MEK-ERK pathway by upregulating KIF15 to promote the malignant progression of liver cancer |
| title_sort | psmd12 promotes the activation of the mek erk pathway by upregulating kif15 to promote the malignant progression of liver cancer |
| topic | psmd12 kif15 mek erk hepatocellular carcinoma |
| url | http://dx.doi.org/10.1080/15384047.2022.2125260 |
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