Cytotoxic and Genotoxic Evaluation of Biosynthesized Silver Nanoparticles Using <i>Moringa oleifera</i> on MCF-7 and HUVEC Cell Lines
Nowadays, green synthesized nanoparticles (NPs) are extensively investigated to explore their biological potential. They are being explored to treat different infectious and cancerous diseases. Therefore, the current study was designed to evaluate the cytotoxic and genotoxic effects of biosynthesize...
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MDPI AG
2022-05-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/10/1293 |
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| author | Hatice Alkan İbrahim Hakkı Ciğerci Muhammad Muddassir Ali Omer Hazman Recep Liman Florica Colă Elena Bonciu |
| author_facet | Hatice Alkan İbrahim Hakkı Ciğerci Muhammad Muddassir Ali Omer Hazman Recep Liman Florica Colă Elena Bonciu |
| author_sort | Hatice Alkan |
| collection | DOAJ |
| description | Nowadays, green synthesized nanoparticles (NPs) are extensively investigated to explore their biological potential. They are being explored to treat different infectious and cancerous diseases. Therefore, the current study was designed to evaluate the cytotoxic and genotoxic effects of biosynthesized silver nanoparticles (AgNPs) from the medicinal plant <i>Moringa oleifera</i> on breast cancer (MCF-7) and HUVEC (human umbilical vein endothelial cells) cell lines. <i>M. oleifera</i>-mediated AgNPs were synthesized from the <i>M. oleifera</i> extract (MOE) and then characterized through the use of a scanning electron microscope (SEM), X-ray diffraction (XRD) and UV–vis spectrophotometer. Biosynthesized AgNPs and MOE were employed on MCF-7 and HUVEC cell lines to evaluate their cytotoxic and genotoxic effects. More cytotoxic effects were observed by AgNPs and MOE on MCF-7 cell lines. The IC<sub>50</sub> for biosynthesized AgNPs was found to be 5 μg/mL. DNA damage was also observed by the MOE and AgNPs on MCF-7 cell lines. However, non-significant DNA damage was observed by MOE and AgNPs on HUVEC cell lines. The findings of the current study revealed the cytotoxic and genotoxic effects of biosynthesized AgNPs on MCF-7 cell lines. However, these AgNPs were considered safe for normal HUVEC cell lines. |
| first_indexed | 2024-03-10T03:03:36Z |
| format | Article |
| id | doaj.art-4819da28ec094c0f816a838fd19f38d2 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T03:03:36Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-4819da28ec094c0f816a838fd19f38d22023-11-23T12:41:52ZengMDPI AGPlants2223-77472022-05-011110129310.3390/plants11101293Cytotoxic and Genotoxic Evaluation of Biosynthesized Silver Nanoparticles Using <i>Moringa oleifera</i> on MCF-7 and HUVEC Cell LinesHatice Alkan0İbrahim Hakkı Ciğerci1Muhammad Muddassir Ali2Omer Hazman3Recep Liman4Florica Colă5Elena Bonciu6Faculty of Science and Literature, Molecular Biology and Genetics Department, Afyon Kocatepe University, Afyonkarahisar 03200, TurkeyFaculty of Science and Literature, Molecular Biology and Genetics Department, Afyon Kocatepe University, Afyonkarahisar 03200, TurkeyInstitute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences Lahore, Punjab 54000, PakistanFaculty of Science and Literature, Department of Chemistry, Afyon Kocatepe University, Afyonkarahisar 03200, TurkeyFaculty of Arts and Sciences, Molecular Biology and Genetics Department, Uşak University, 1 Eylül Campus, Uşak 64300, TurkeyFaculty of Agronomy, Department of Agricultural and Forestry Technology, University of Craiova, 13 A.I. Cuza Street, 200585 Craiova, RomaniaFaculty of Agronomy, Department of Agricultural and Forestry Technology, University of Craiova, 13 A.I. Cuza Street, 200585 Craiova, RomaniaNowadays, green synthesized nanoparticles (NPs) are extensively investigated to explore their biological potential. They are being explored to treat different infectious and cancerous diseases. Therefore, the current study was designed to evaluate the cytotoxic and genotoxic effects of biosynthesized silver nanoparticles (AgNPs) from the medicinal plant <i>Moringa oleifera</i> on breast cancer (MCF-7) and HUVEC (human umbilical vein endothelial cells) cell lines. <i>M. oleifera</i>-mediated AgNPs were synthesized from the <i>M. oleifera</i> extract (MOE) and then characterized through the use of a scanning electron microscope (SEM), X-ray diffraction (XRD) and UV–vis spectrophotometer. Biosynthesized AgNPs and MOE were employed on MCF-7 and HUVEC cell lines to evaluate their cytotoxic and genotoxic effects. More cytotoxic effects were observed by AgNPs and MOE on MCF-7 cell lines. The IC<sub>50</sub> for biosynthesized AgNPs was found to be 5 μg/mL. DNA damage was also observed by the MOE and AgNPs on MCF-7 cell lines. However, non-significant DNA damage was observed by MOE and AgNPs on HUVEC cell lines. The findings of the current study revealed the cytotoxic and genotoxic effects of biosynthesized AgNPs on MCF-7 cell lines. However, these AgNPs were considered safe for normal HUVEC cell lines.https://www.mdpi.com/2223-7747/11/10/1293DNA damagenanotechnologygreen synthesisMTTcomet assay |
| spellingShingle | Hatice Alkan İbrahim Hakkı Ciğerci Muhammad Muddassir Ali Omer Hazman Recep Liman Florica Colă Elena Bonciu Cytotoxic and Genotoxic Evaluation of Biosynthesized Silver Nanoparticles Using <i>Moringa oleifera</i> on MCF-7 and HUVEC Cell Lines Plants DNA damage nanotechnology green synthesis MTT comet assay |
| title | Cytotoxic and Genotoxic Evaluation of Biosynthesized Silver Nanoparticles Using <i>Moringa oleifera</i> on MCF-7 and HUVEC Cell Lines |
| title_full | Cytotoxic and Genotoxic Evaluation of Biosynthesized Silver Nanoparticles Using <i>Moringa oleifera</i> on MCF-7 and HUVEC Cell Lines |
| title_fullStr | Cytotoxic and Genotoxic Evaluation of Biosynthesized Silver Nanoparticles Using <i>Moringa oleifera</i> on MCF-7 and HUVEC Cell Lines |
| title_full_unstemmed | Cytotoxic and Genotoxic Evaluation of Biosynthesized Silver Nanoparticles Using <i>Moringa oleifera</i> on MCF-7 and HUVEC Cell Lines |
| title_short | Cytotoxic and Genotoxic Evaluation of Biosynthesized Silver Nanoparticles Using <i>Moringa oleifera</i> on MCF-7 and HUVEC Cell Lines |
| title_sort | cytotoxic and genotoxic evaluation of biosynthesized silver nanoparticles using i moringa oleifera i on mcf 7 and huvec cell lines |
| topic | DNA damage nanotechnology green synthesis MTT comet assay |
| url | https://www.mdpi.com/2223-7747/11/10/1293 |
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