Acute and sub-chronic toxicological evaluation of ethanol extract of Solanum trilobatum Linn.
Background and objectives: Solanum trilobatum plant parts such as berries and flowers are normally used for the treatment of respiratory illnesses. The toxicity profile of the plant and its parts are not clear. Hence, the present study was planned to investigate the toxicological effects of ethanol...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Iranian Society of Pharmacognosy
2018-01-01
|
| Series: | Research Journal of Pharmacognosy |
| Subjects: | |
| Online Access: | http://www.rjpharmacognosy.ir/article_54419_c4e4733302e9e12f42771a6e43cf4f43.pdf |
| _version_ | 1811273724186329088 |
|---|---|
| author | S. Parasuraman* L. Yu Ren B. Lau Chik Chuon S. Wong Kah Yee |
| author_facet | S. Parasuraman* L. Yu Ren B. Lau Chik Chuon S. Wong Kah Yee |
| author_sort | S. Parasuraman* |
| collection | DOAJ |
| description | Background and objectives: Solanum trilobatum plant parts such as berries and flowers are normally used for the treatment of respiratory illnesses. The toxicity profile of the plant and its parts are not clear. Hence, the present study was planned to investigate the toxicological effects of ethanol extract of leaves of S. trilobatum (EEST) using acute and sub-chronic toxicological methods in Sprague-Dawley (SD) rats. Method: Leaves ofS. trilobatum were extracted with ethanol using hot percolation method. Acute and sub-chronic oral toxic effects of EEST were tested in SD rats. Acute toxicity testing was carried out as per guidelines set by OECD. In sub-chronic toxicity testing, animals were treated with 100, 200 and 400 mg/kg EEST for 30 days. During the study, the animals were monitored for changes in their behaviour at regular intervals. At the end of the study, blood sample was collected from all animals for biochemical analysis, they were sacrificed and organs such as brain, lung, liver and kidney were collected for histopathological analysis. Part of the brain was used for estimation of dopamine and the remaining tissue was used for histopathological analysis. Results: In acute toxicity testing, EEST did not show mortality up to 2000 mg/kg. In sub-chronic toxicity testing, EEST at 200 mg/kg and above doses caused cannibalism. At the end of the study, EEST decreased locomotor action and immobilization time. Histopathological analysis showed mild to moderate toxicity in 400 mg/kg treated animals and no significant changes were observed in biochemical parameters compared to control group. Conclusion: The present study concluded that, EEST exerted mild to moderate toxic effects on rodents. EEST caused cannibalism, increased the dopamine level in brain and histopathological alterations in lungs, liver and kidneys. |
| first_indexed | 2024-04-12T23:04:32Z |
| format | Article |
| id | doaj.art-481a04bead3a410c826edec35aa0d038 |
| institution | Directory Open Access Journal |
| issn | 2345-5977 2345-5977 |
| language | English |
| last_indexed | 2024-04-12T23:04:32Z |
| publishDate | 2018-01-01 |
| publisher | Iranian Society of Pharmacognosy |
| record_format | Article |
| series | Research Journal of Pharmacognosy |
| spelling | doaj.art-481a04bead3a410c826edec35aa0d0382022-12-22T03:12:56ZengIranian Society of PharmacognosyResearch Journal of Pharmacognosy2345-59772345-59772018-01-0151132154419Acute and sub-chronic toxicological evaluation of ethanol extract of Solanum trilobatum Linn.S. Parasuraman*0L. Yu Ren1B. Lau Chik Chuon2S. Wong Kah Yee3Unit of Pharmacology, Faculty of Pharmacy, AIMST University, Bedong, Kedah, Malaysia.Unit of Pharmacology, Faculty of Pharmacy, AIMST University, Bedong, Kedah, Malaysia.Unit of Pharmacology, Faculty of Pharmacy, AIMST University, Bedong, Kedah, Malaysia.Unit of Pharmacology, Faculty of Pharmacy, AIMST University, Bedong, Kedah, Malaysia.Background and objectives: Solanum trilobatum plant parts such as berries and flowers are normally used for the treatment of respiratory illnesses. The toxicity profile of the plant and its parts are not clear. Hence, the present study was planned to investigate the toxicological effects of ethanol extract of leaves of S. trilobatum (EEST) using acute and sub-chronic toxicological methods in Sprague-Dawley (SD) rats. Method: Leaves ofS. trilobatum were extracted with ethanol using hot percolation method. Acute and sub-chronic oral toxic effects of EEST were tested in SD rats. Acute toxicity testing was carried out as per guidelines set by OECD. In sub-chronic toxicity testing, animals were treated with 100, 200 and 400 mg/kg EEST for 30 days. During the study, the animals were monitored for changes in their behaviour at regular intervals. At the end of the study, blood sample was collected from all animals for biochemical analysis, they were sacrificed and organs such as brain, lung, liver and kidney were collected for histopathological analysis. Part of the brain was used for estimation of dopamine and the remaining tissue was used for histopathological analysis. Results: In acute toxicity testing, EEST did not show mortality up to 2000 mg/kg. In sub-chronic toxicity testing, EEST at 200 mg/kg and above doses caused cannibalism. At the end of the study, EEST decreased locomotor action and immobilization time. Histopathological analysis showed mild to moderate toxicity in 400 mg/kg treated animals and no significant changes were observed in biochemical parameters compared to control group. Conclusion: The present study concluded that, EEST exerted mild to moderate toxic effects on rodents. EEST caused cannibalism, increased the dopamine level in brain and histopathological alterations in lungs, liver and kidneys.http://www.rjpharmacognosy.ir/article_54419_c4e4733302e9e12f42771a6e43cf4f43.pdfcannibalismlocomotor activitySolanum trilobatumSprague-Dawley ratsToxicity |
| spellingShingle | S. Parasuraman* L. Yu Ren B. Lau Chik Chuon S. Wong Kah Yee Acute and sub-chronic toxicological evaluation of ethanol extract of Solanum trilobatum Linn. Research Journal of Pharmacognosy cannibalism locomotor activity Solanum trilobatum Sprague-Dawley rats Toxicity |
| title | Acute and sub-chronic toxicological evaluation of ethanol extract of Solanum trilobatum Linn. |
| title_full | Acute and sub-chronic toxicological evaluation of ethanol extract of Solanum trilobatum Linn. |
| title_fullStr | Acute and sub-chronic toxicological evaluation of ethanol extract of Solanum trilobatum Linn. |
| title_full_unstemmed | Acute and sub-chronic toxicological evaluation of ethanol extract of Solanum trilobatum Linn. |
| title_short | Acute and sub-chronic toxicological evaluation of ethanol extract of Solanum trilobatum Linn. |
| title_sort | acute and sub chronic toxicological evaluation of ethanol extract of solanum trilobatum linn |
| topic | cannibalism locomotor activity Solanum trilobatum Sprague-Dawley rats Toxicity |
| url | http://www.rjpharmacognosy.ir/article_54419_c4e4733302e9e12f42771a6e43cf4f43.pdf |
| work_keys_str_mv | AT sparasuraman acuteandsubchronictoxicologicalevaluationofethanolextractofsolanumtrilobatumlinn AT lyuren acuteandsubchronictoxicologicalevaluationofethanolextractofsolanumtrilobatumlinn AT blauchikchuon acuteandsubchronictoxicologicalevaluationofethanolextractofsolanumtrilobatumlinn AT swongkahyee acuteandsubchronictoxicologicalevaluationofethanolextractofsolanumtrilobatumlinn |