Assessment of the TRX2p-yEGFP Biosensor to Monitor the Redox Response of an Industrial Xylose-Fermenting <i>Saccharomyces cerevisiae</i> Strain during Propagation and Fermentation
The commercial production of bioethanol from lignocellulosic biomass such as wheat straw requires utilizing a microorganism that can withstand all the stressors encountered in the process while fermenting all the sugars in the biomass. Therefore, it is essential to develop tools for monitoring and c...
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MDPI AG
2023-05-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/9/6/630 |
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| author | Raquel Perruca Foncillas Miguel Sanchis Sebastiá Ola Wallberg Magnus Carlquist Marie F. Gorwa-Grauslund |
| author_facet | Raquel Perruca Foncillas Miguel Sanchis Sebastiá Ola Wallberg Magnus Carlquist Marie F. Gorwa-Grauslund |
| author_sort | Raquel Perruca Foncillas |
| collection | DOAJ |
| description | The commercial production of bioethanol from lignocellulosic biomass such as wheat straw requires utilizing a microorganism that can withstand all the stressors encountered in the process while fermenting all the sugars in the biomass. Therefore, it is essential to develop tools for monitoring and controlling the cellular fitness during both cell propagation and sugar fermentation to ethanol. In the present study, on-line flow cytometry was adopted to assess the response of the biosensor TRX2p-yEGFP for redox imbalance in an industrial xylose-fermenting strain of <i>Saccharomyces cerevisiae</i> during cell propagation and the following fermentation of wheat-straw hydrolysate. Rapid and transient induction of the sensor was recorded upon exposure to furfural and wheat straw hydrolysate containing up to 3.8 g/L furfural. During the fermentation step, the induction rate of the sensor was also found to correlate to the initial ethanol production rate, highlighting the relevance of redox monitoring and the potential of the presented tool to assess the ethanol production rate in hydrolysates. Three different propagation strategies were also compared, and it was confirmed that pre-exposure to hydrolysate during propagation remains the most efficient method for high ethanol productivity in the following wheat-straw hydrolysate fermentations. |
| first_indexed | 2024-03-11T02:16:16Z |
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| id | doaj.art-481a51fb453346a9a518c320c72a5dba |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-11T02:16:16Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-481a51fb453346a9a518c320c72a5dba2023-11-18T11:08:39ZengMDPI AGJournal of Fungi2309-608X2023-05-019663010.3390/jof9060630Assessment of the TRX2p-yEGFP Biosensor to Monitor the Redox Response of an Industrial Xylose-Fermenting <i>Saccharomyces cerevisiae</i> Strain during Propagation and FermentationRaquel Perruca Foncillas0Miguel Sanchis Sebastiá1Ola Wallberg2Magnus Carlquist3Marie F. Gorwa-Grauslund4Applied Microbiology, Department of Chemistry, Lund University, P.O. Box 124, SE-221 00 Lund, SwedenDepartment of Chemical Engineering, Lund University, P.O. Box 124, SE-221 00 Lund, SwedenDepartment of Chemical Engineering, Lund University, P.O. Box 124, SE-221 00 Lund, SwedenApplied Microbiology, Department of Chemistry, Lund University, P.O. Box 124, SE-221 00 Lund, SwedenApplied Microbiology, Department of Chemistry, Lund University, P.O. Box 124, SE-221 00 Lund, SwedenThe commercial production of bioethanol from lignocellulosic biomass such as wheat straw requires utilizing a microorganism that can withstand all the stressors encountered in the process while fermenting all the sugars in the biomass. Therefore, it is essential to develop tools for monitoring and controlling the cellular fitness during both cell propagation and sugar fermentation to ethanol. In the present study, on-line flow cytometry was adopted to assess the response of the biosensor TRX2p-yEGFP for redox imbalance in an industrial xylose-fermenting strain of <i>Saccharomyces cerevisiae</i> during cell propagation and the following fermentation of wheat-straw hydrolysate. Rapid and transient induction of the sensor was recorded upon exposure to furfural and wheat straw hydrolysate containing up to 3.8 g/L furfural. During the fermentation step, the induction rate of the sensor was also found to correlate to the initial ethanol production rate, highlighting the relevance of redox monitoring and the potential of the presented tool to assess the ethanol production rate in hydrolysates. Three different propagation strategies were also compared, and it was confirmed that pre-exposure to hydrolysate during propagation remains the most efficient method for high ethanol productivity in the following wheat-straw hydrolysate fermentations.https://www.mdpi.com/2309-608X/9/6/630on-line flow cytometryredox imbalanceTRX2p-GFPyeast propagationlignocellulosic bioethanolwheat-straw hydrolysate |
| spellingShingle | Raquel Perruca Foncillas Miguel Sanchis Sebastiá Ola Wallberg Magnus Carlquist Marie F. Gorwa-Grauslund Assessment of the TRX2p-yEGFP Biosensor to Monitor the Redox Response of an Industrial Xylose-Fermenting <i>Saccharomyces cerevisiae</i> Strain during Propagation and Fermentation Journal of Fungi on-line flow cytometry redox imbalance TRX2p-GFP yeast propagation lignocellulosic bioethanol wheat-straw hydrolysate |
| title | Assessment of the TRX2p-yEGFP Biosensor to Monitor the Redox Response of an Industrial Xylose-Fermenting <i>Saccharomyces cerevisiae</i> Strain during Propagation and Fermentation |
| title_full | Assessment of the TRX2p-yEGFP Biosensor to Monitor the Redox Response of an Industrial Xylose-Fermenting <i>Saccharomyces cerevisiae</i> Strain during Propagation and Fermentation |
| title_fullStr | Assessment of the TRX2p-yEGFP Biosensor to Monitor the Redox Response of an Industrial Xylose-Fermenting <i>Saccharomyces cerevisiae</i> Strain during Propagation and Fermentation |
| title_full_unstemmed | Assessment of the TRX2p-yEGFP Biosensor to Monitor the Redox Response of an Industrial Xylose-Fermenting <i>Saccharomyces cerevisiae</i> Strain during Propagation and Fermentation |
| title_short | Assessment of the TRX2p-yEGFP Biosensor to Monitor the Redox Response of an Industrial Xylose-Fermenting <i>Saccharomyces cerevisiae</i> Strain during Propagation and Fermentation |
| title_sort | assessment of the trx2p yegfp biosensor to monitor the redox response of an industrial xylose fermenting i saccharomyces cerevisiae i strain during propagation and fermentation |
| topic | on-line flow cytometry redox imbalance TRX2p-GFP yeast propagation lignocellulosic bioethanol wheat-straw hydrolysate |
| url | https://www.mdpi.com/2309-608X/9/6/630 |
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