The Interaction between Mitochondrial Oxidative Stress and Gut Microbiota in the Cardiometabolic Consequences in Diet-Induced Obese Rats
Background: The objective of this study is to determine the role of mitochondrial oxidative stress in the dysbiosis associated with a high fat diet in rats. In addition, the impact of gut microbiota (GM) in the cardiometabolic consequences of diet-induced obesity in rats has been evaluated. Methods:...
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MDPI AG
2020-07-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/9/7/640 |
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| author | Adriana Ortega-Hernández Ernesto Martínez-Martínez Ruben Gómez-Gordo Natalia López-Andrés Amaya Fernández-Celis Beatriz Gutiérrrez-Miranda María Luisa Nieto Teresa Alarcón Claudio Alba Dulcenombre Gómez-Garre Victoria Cachofeiro |
| author_facet | Adriana Ortega-Hernández Ernesto Martínez-Martínez Ruben Gómez-Gordo Natalia López-Andrés Amaya Fernández-Celis Beatriz Gutiérrrez-Miranda María Luisa Nieto Teresa Alarcón Claudio Alba Dulcenombre Gómez-Garre Victoria Cachofeiro |
| author_sort | Adriana Ortega-Hernández |
| collection | DOAJ |
| description | Background: The objective of this study is to determine the role of mitochondrial oxidative stress in the dysbiosis associated with a high fat diet in rats. In addition, the impact of gut microbiota (GM) in the cardiometabolic consequences of diet-induced obesity in rats has been evaluated. Methods: Male Wistar rats were fed either a high fat diet (HFD) or a control (CT) one for 6 weeks. At the third week, one-half of the animals of each group were treated with the mitochondrial antioxidant MitoTempo (MT; 0.7 mgKg<sup>−1</sup>day<sup>−1</sup> i.p). Results: Animals fed an HFD showed a lower microbiota evenness and diversity in comparison to CT rats. This dysbiosis is characterized by a decrease in <i>Firmicutes/Bacteroidetes</i> ratio and relevant changes at family and genera compared with the CT group. This was accompanied by a reduction in colonic mucin-secreting goblet cells. These changes were reversed by MT treatment. The abundance of certain genera could also be relevant in the metabolic consequences of obesity, as well as in the occurrence of cardiac fibrosis associated with obesity. Conclusions: These results support an interaction between GM and mitochondrial oxidative stress and its relation with development of cardiac fibrosis, suggesting new approaches in the management of obesity-related cardiometabolic consequences. |
| first_indexed | 2024-03-10T18:19:42Z |
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| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T18:19:42Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-481bd102d9c44a15bfebf35ba734953e2023-11-20T07:23:52ZengMDPI AGAntioxidants2076-39212020-07-019764010.3390/antiox9070640The Interaction between Mitochondrial Oxidative Stress and Gut Microbiota in the Cardiometabolic Consequences in Diet-Induced Obese RatsAdriana Ortega-Hernández0Ernesto Martínez-Martínez1Ruben Gómez-Gordo2Natalia López-Andrés3Amaya Fernández-Celis4Beatriz Gutiérrrez-Miranda5María Luisa Nieto6Teresa Alarcón7Claudio Alba8Dulcenombre Gómez-Garre9Victoria Cachofeiro10Vascular Biology and Microbiota Laboratory, Hospital Clínico San Carlos-Instituto de Investigación Sanitaria San Carlos (IdISSC), 28040-Madrid, SpainCiber de Enfermedades Cardiovasculares (CIBERCV), Instituto de Salud Carlos III, 28029-Madrid, SpainVascular Biology and Microbiota Laboratory, Hospital Clínico San Carlos-Instituto de Investigación Sanitaria San Carlos (IdISSC), 28040-Madrid, SpainCardiovascular Translational Research, Navarrabiomed, Complejo Hospitalario de Navarra (CHN), Universidad Pública de Navarra (UPNA), IdiSNA, 31008 Pamplona, SpainCardiovascular Translational Research, Navarrabiomed, Complejo Hospitalario de Navarra (CHN), Universidad Pública de Navarra (UPNA), IdiSNA, 31008 Pamplona, SpainInstituto de Biología y Genética Molecular, CSIC-Universidad de Valladolid, 47003 Valladolid, SpainCiber de Enfermedades Cardiovasculares (CIBERCV), Instituto de Salud Carlos III, 28029-Madrid, SpainServicio de Microbiología, Hospital Universitario de La Princesa, Instituto de Investigación Sanitaria La Princesa, Departamento de Medicina Preventiva, Salud Pública y Microbiología, Facultad de Medicina, Universidad Autónoma de Madrid, 28006 Madrid, SpainSección Departamental de Farmacia Galénica y Tecnología Alimentaria, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040-Madrid, SpainVascular Biology and Microbiota Laboratory, Hospital Clínico San Carlos-Instituto de Investigación Sanitaria San Carlos (IdISSC), 28040-Madrid, SpainCiber de Enfermedades Cardiovasculares (CIBERCV), Instituto de Salud Carlos III, 28029-Madrid, SpainBackground: The objective of this study is to determine the role of mitochondrial oxidative stress in the dysbiosis associated with a high fat diet in rats. In addition, the impact of gut microbiota (GM) in the cardiometabolic consequences of diet-induced obesity in rats has been evaluated. Methods: Male Wistar rats were fed either a high fat diet (HFD) or a control (CT) one for 6 weeks. At the third week, one-half of the animals of each group were treated with the mitochondrial antioxidant MitoTempo (MT; 0.7 mgKg<sup>−1</sup>day<sup>−1</sup> i.p). Results: Animals fed an HFD showed a lower microbiota evenness and diversity in comparison to CT rats. This dysbiosis is characterized by a decrease in <i>Firmicutes/Bacteroidetes</i> ratio and relevant changes at family and genera compared with the CT group. This was accompanied by a reduction in colonic mucin-secreting goblet cells. These changes were reversed by MT treatment. The abundance of certain genera could also be relevant in the metabolic consequences of obesity, as well as in the occurrence of cardiac fibrosis associated with obesity. Conclusions: These results support an interaction between GM and mitochondrial oxidative stress and its relation with development of cardiac fibrosis, suggesting new approaches in the management of obesity-related cardiometabolic consequences.https://www.mdpi.com/2076-3921/9/7/640cardiac fibrosisinsulin resistancemicrobiotamucinsobesity |
| spellingShingle | Adriana Ortega-Hernández Ernesto Martínez-Martínez Ruben Gómez-Gordo Natalia López-Andrés Amaya Fernández-Celis Beatriz Gutiérrrez-Miranda María Luisa Nieto Teresa Alarcón Claudio Alba Dulcenombre Gómez-Garre Victoria Cachofeiro The Interaction between Mitochondrial Oxidative Stress and Gut Microbiota in the Cardiometabolic Consequences in Diet-Induced Obese Rats Antioxidants cardiac fibrosis insulin resistance microbiota mucins obesity |
| title | The Interaction between Mitochondrial Oxidative Stress and Gut Microbiota in the Cardiometabolic Consequences in Diet-Induced Obese Rats |
| title_full | The Interaction between Mitochondrial Oxidative Stress and Gut Microbiota in the Cardiometabolic Consequences in Diet-Induced Obese Rats |
| title_fullStr | The Interaction between Mitochondrial Oxidative Stress and Gut Microbiota in the Cardiometabolic Consequences in Diet-Induced Obese Rats |
| title_full_unstemmed | The Interaction between Mitochondrial Oxidative Stress and Gut Microbiota in the Cardiometabolic Consequences in Diet-Induced Obese Rats |
| title_short | The Interaction between Mitochondrial Oxidative Stress and Gut Microbiota in the Cardiometabolic Consequences in Diet-Induced Obese Rats |
| title_sort | interaction between mitochondrial oxidative stress and gut microbiota in the cardiometabolic consequences in diet induced obese rats |
| topic | cardiac fibrosis insulin resistance microbiota mucins obesity |
| url | https://www.mdpi.com/2076-3921/9/7/640 |
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