Aquaphotomics Research of Cold Stress in Soybean Cultivars with Different Stress Tolerance Ability: Early Detection of Cold Stress Response
The development of non-destructive methods for early detection of cold stress of plants and the identification of cold-tolerant cultivars is highly needed in crop breeding programs. Current methods are either destructive, time-consuming or imprecise. In this study, soybean leaves’ spectra were acqui...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-01-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/27/3/744 |
| _version_ | 1827659781219811328 |
|---|---|
| author | Jelena Muncan Balasooriya Mudiyanselage Siriwijaya Jinendra Shinichiro Kuroki Roumiana Tsenkova |
| author_facet | Jelena Muncan Balasooriya Mudiyanselage Siriwijaya Jinendra Shinichiro Kuroki Roumiana Tsenkova |
| author_sort | Jelena Muncan |
| collection | DOAJ |
| description | The development of non-destructive methods for early detection of cold stress of plants and the identification of cold-tolerant cultivars is highly needed in crop breeding programs. Current methods are either destructive, time-consuming or imprecise. In this study, soybean leaves’ spectra were acquired in the near infrared (NIR) range (588–1025 nm) from five cultivars genetically engineered to have different levels of cold stress tolerance. The spectra were acquired at the optimal growing temperature 27 °C and when the temperature was decreased to 22 °C. In this paper, we report the results of the aquaphotomics analysis performed with the objective of understanding the role of the water molecular system in the early cold stress response of all cultivars. The raw spectra and the results of Principal Component Analysis, Soft Independent Modeling of Class Analogies and aquagrams showed consistent evidence of huge differences in the NIR spectral profiles of all cultivars under normal and mild cold stress conditions. The SIMCA discrimination between the plants before and after stress was achieved with 100% accuracy. The interpretation of spectral patterns before and after cold stress revealed major changes in the water molecular structure of the soybean leaves, altered carbohydrate and oxidative metabolism. Specific water molecular structures in the leaves of soybean cultivars were found to be highly sensitive to the temperature, showing their crucial role in the cold stress response. The results also indicated the existence of differences in the cold stress response of different cultivars, which will be a topic of further research. |
| first_indexed | 2024-03-09T23:29:07Z |
| format | Article |
| id | doaj.art-4823a44baff9465d93685ac46c2f7a3e |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T23:29:07Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-4823a44baff9465d93685ac46c2f7a3e2023-11-23T17:11:47ZengMDPI AGMolecules1420-30492022-01-0127374410.3390/molecules27030744Aquaphotomics Research of Cold Stress in Soybean Cultivars with Different Stress Tolerance Ability: Early Detection of Cold Stress ResponseJelena Muncan0Balasooriya Mudiyanselage Siriwijaya Jinendra1Shinichiro Kuroki2Roumiana Tsenkova3Aquaphotomics Research Department, Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, JapanDepartment of Agricultural Engineering, Faculty of Agriculture, University of Ruhuna, Mapalana 81100, Sri LankaLaboratory for Information Engineering of Bioproduction, Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, JapanAquaphotomics Research Department, Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, JapanThe development of non-destructive methods for early detection of cold stress of plants and the identification of cold-tolerant cultivars is highly needed in crop breeding programs. Current methods are either destructive, time-consuming or imprecise. In this study, soybean leaves’ spectra were acquired in the near infrared (NIR) range (588–1025 nm) from five cultivars genetically engineered to have different levels of cold stress tolerance. The spectra were acquired at the optimal growing temperature 27 °C and when the temperature was decreased to 22 °C. In this paper, we report the results of the aquaphotomics analysis performed with the objective of understanding the role of the water molecular system in the early cold stress response of all cultivars. The raw spectra and the results of Principal Component Analysis, Soft Independent Modeling of Class Analogies and aquagrams showed consistent evidence of huge differences in the NIR spectral profiles of all cultivars under normal and mild cold stress conditions. The SIMCA discrimination between the plants before and after stress was achieved with 100% accuracy. The interpretation of spectral patterns before and after cold stress revealed major changes in the water molecular structure of the soybean leaves, altered carbohydrate and oxidative metabolism. Specific water molecular structures in the leaves of soybean cultivars were found to be highly sensitive to the temperature, showing their crucial role in the cold stress response. The results also indicated the existence of differences in the cold stress response of different cultivars, which will be a topic of further research.https://www.mdpi.com/1420-3049/27/3/744cold stressstress tolerancesoybeanwaternear infrared spectroscopyaquaphotomics |
| spellingShingle | Jelena Muncan Balasooriya Mudiyanselage Siriwijaya Jinendra Shinichiro Kuroki Roumiana Tsenkova Aquaphotomics Research of Cold Stress in Soybean Cultivars with Different Stress Tolerance Ability: Early Detection of Cold Stress Response Molecules cold stress stress tolerance soybean water near infrared spectroscopy aquaphotomics |
| title | Aquaphotomics Research of Cold Stress in Soybean Cultivars with Different Stress Tolerance Ability: Early Detection of Cold Stress Response |
| title_full | Aquaphotomics Research of Cold Stress in Soybean Cultivars with Different Stress Tolerance Ability: Early Detection of Cold Stress Response |
| title_fullStr | Aquaphotomics Research of Cold Stress in Soybean Cultivars with Different Stress Tolerance Ability: Early Detection of Cold Stress Response |
| title_full_unstemmed | Aquaphotomics Research of Cold Stress in Soybean Cultivars with Different Stress Tolerance Ability: Early Detection of Cold Stress Response |
| title_short | Aquaphotomics Research of Cold Stress in Soybean Cultivars with Different Stress Tolerance Ability: Early Detection of Cold Stress Response |
| title_sort | aquaphotomics research of cold stress in soybean cultivars with different stress tolerance ability early detection of cold stress response |
| topic | cold stress stress tolerance soybean water near infrared spectroscopy aquaphotomics |
| url | https://www.mdpi.com/1420-3049/27/3/744 |
| work_keys_str_mv | AT jelenamuncan aquaphotomicsresearchofcoldstressinsoybeancultivarswithdifferentstresstoleranceabilityearlydetectionofcoldstressresponse AT balasooriyamudiyanselagesiriwijayajinendra aquaphotomicsresearchofcoldstressinsoybeancultivarswithdifferentstresstoleranceabilityearlydetectionofcoldstressresponse AT shinichirokuroki aquaphotomicsresearchofcoldstressinsoybeancultivarswithdifferentstresstoleranceabilityearlydetectionofcoldstressresponse AT roumianatsenkova aquaphotomicsresearchofcoldstressinsoybeancultivarswithdifferentstresstoleranceabilityearlydetectionofcoldstressresponse |